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Expression of virulence and antimicrobial resistance genes among Escherichia coli clinical isolates from blood and stool samples
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Objective:

As major nosocomial pathogens, Escherichia coli isolates exhibit antibiotic resistance and also express adhesive structures and antibiotic resistance genes. The objective of this study was the comparison of virulence gene expression of extended-spectrum beta-lactamase (ESBL)-producing E. coli between blood and stool samples.

Methods:

In this study, 20 E. coli clinical isolates (10 ESBL-producers including 5 from blood, 5 from stool samples and 10 non-ESBL-producer strains) were included. The existence of fimA, kpsMII and cdt (adhesives and toxin), acr-ab (efflux-encoding) and bla CTX-M1 genes were confirmed by PCR. The quantitative real-time PCR was performed for evaluation of gene expression.

Results:

ESBL-producing E. coli isolates from stool samples could express fimA, kpsMII and cdt genes significantly higher than blood samples, whereas those isolates from blood samples significantly expressed the acr-ab (efflux-encoding) genes. In addition, the bla CTXM1 gene was expressed among isolates from stool samples significantly higher (P = 0.022) than those from blood samples according to the analysis of variance (ANOVA) test. In addition, among non-ESBL-producers, the expression of fimA, kpsMII and cdt genes was significantly lower than ESBL-producing isolates from blood samples, but not significantly different than those from stool samples. Moreover, the expression of acr-ab genes was significantly lower than those from stool samples.

Conclusion:

The results exhibited that the expression of virulence genes among clinical isolates of E. coli is not the same or similar in various conditions or from various clinical origins. Thus determining the profile of gene expression in each of clinical situations can be helpful in tracking the infectious pathogens. ESBL-producing strains possibly have regulatory factors for inducing higher virulence gene expression.

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Publication Date
Mon May 29 2017
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
APPLICATION OF TQM REQUIREMENTS AND THEIR RELATIONSHIP TO ORGANIZATIONAL PERFORMANCE FROM THE POINT OF VIEW OF THE INTERNAL CUSTOMER/ COMPARATIVE STUDY.: APPLICATION OF TQM REQUIREMENTS AND THEIR RELATIONSHIP TO ORGANIZATIONAL PERFORMANCE FROM THE POINT OF VIEW OF THE INTERNAL CUSTOMER/ COMPARATIVE STUDY.
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This study aims to Statement of the relationship between Total Quality Management philosophy and Organizational performance from the point of view of the internal customer. A comparison has been made between two companies, one of which applies the requirements of TQM well and the other does not apply these requirements as the (General Company for Electrical Industries/ Diyala) and (General Company for Electrical Industries/ Baghdad) to conduct the search, During the questionnaire prepared for this purpose and distributed to a sample of 30 employees in the General Company for Electric Industries/ Diyala and (20) employees of the General Company for Electrical Industries/ Baghdad. Their answers were analyzed using a simple correlation coef

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Publication Date
Sat Sep 30 2023
Journal Name
Journal Of The College Of Education For Women
Challenges of Spreading the Culture of Peace in Iraq from the point of view of Leaders and Intellectuals Elites
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The research problem lies in identifying the challenges facing spreading the culture of peace in Iraq, and the most important factors of its instability from the point of view of leaders and intellectual elites.The research derives its importance from the fact that the peace imposed by justice leads to a stable Iraq and a prosperous future.

The study aims to identify the most prominent challenges and obstacles created by wars and faced the process of spreading the culture of peace and instability in Iraq, and to shed light on the reasons leading to the employment of the capabilities of community leaders in spreading the culture of peace.The objective of the current research is to identify the challenges of spreading the culture o

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Publication Date
Tue Dec 11 2018
Journal Name
Iraqi National Journal Of Nursing Specialties
Episiotomy preventing approaches among midwives in Kurdistan region
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Objectives: This study aimed to findout approaches which used by midwives for avoiding episiotomy and assess their knowledge regarding complications of episiotomy in three main cities of Kurdistan region. Methodology: A cross-sectional study was conducted between 1st January and 15th April 2014 in the three biggest maternity teaching hospitals. The study population included all the midwives (n=53) working in delivery rooms. Data were collected through interview with midwives. Results: The Midwives stated the followings as complications of episiotomy: tear into the rectum muscle or rectum itself (52.8%), bleedi

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Publication Date
Wed Jan 09 2019
Journal Name
Journal Of The College Of Education For Women
The Attitudes Toward Extremism Among University Students Baghdad
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The Present study aims to investigate the attitude toward extremism of the university student and to find differences with three variables, gender field of study ,grade), For the purposes of the study on(3) level scale of the attitude towards extremism , and subjected to validity and credibility ,the scale was designed for ( 590) students sample (237) males ,and (357) females Results shown that students has a mild attitude towards extremism compared with the average of the scale attitude towards Religious extremism occupied the number one level ,followed by social extremism and lastly political extremism in Results also shown different in gender (males ,females) with the males having the granter attitude towards extremism as for the othe

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Publication Date
Mon Mar 08 2021
Journal Name
Baghdad Science Journal
Totas serum sialic acid among enteric fever patients
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In this study serum total sailie acid concentration were tested as a scrological marker of discases activity to cvalute the result of the test in the diagonosis oe enteric fever(TSA) was measured in the serum od (50) patines with typhi fever(50)pa-tients

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Publication Date
Wed Jul 06 2022
Journal Name
International Journal Of Early Childhood Special Education (int-jecse)
Imaginative thinking among secondary school students in mathematics
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The research aims at identifying the level of imaginative thinking skills in mathematics among fourth scientific grade students.For this purpose, the researcher adopted the descriptive (survey) approach, as an imaginative thinking test was built and applied to the research sample that consists of (301) students from fourth scientific grade students who are distributed to secondary and Preparatory schools,they are Al-Furat Preparatory, Al-Hindiya Preparatory school and Al-Rajiba Preparatory school , who are affiliated to the Al-Hindiya Education Department of the General Directorate in the Holy Karbala Governorate for the academic year (2021-2022). The imaginative thinking test consisted of (36) items of four alternatives multiple-choice obj

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Publication Date
Mon Mar 01 2021
Journal Name
Journal Of Professional Nursing
Incivility among nursing faculty: A multi-country study
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Publication Date
Mon Feb 28 2022
Journal Name
Journal Of Educational And Psychological Researches
Occupational Stress among Doctors Work in Governmental Hospitals
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The current research aims to identify the occupational stresses of doctors who are working in governmental hospitals according to the variables of gender and career ranking. The researcher adopted a scale to measure the occupational stress of (1088) doctors (561 males and 527 females) working in governmental hospitals. The results have shown that doctors have a high level of professional stress, but there is no significant difference between doctors in terms of gender. However, there were significant differences in favor of novice residents.

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Publication Date
Tue Jun 30 2009
Journal Name
Al-kindy College Medical Journal
Behavioral Disturbances among School Children in Baghdad Province
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Background: Mental health of children has recently been
receiving increased attention in many parts of the world.
However, such attention has been lacking in developing
countries, particularly in the form of systematic research
Aim: The study aimed to find out the frequency of
behavior disorders in a sample of primary school children
and the different social and demographic factors that might
be related to this disorder.
Methods: The study is a cross-sectional one that included
918 primary school pupils in both urban and rural areas of
Baghdad. Rutter's questionnaire teacher's version was used
to assess the pupils, and the demographic data was obtained
by special questionnaire sent to the parents to be

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Publication Date
Wed Mar 15 2023
Journal Name
Al-academy
The Self-Efficacy Level Among Musically Talented Students
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This Study Aimed to Recognize the Self-Efficacy Level Among Musically Talented Students the sample of this study consisted of (85) Musically smart students male and female students in Irbid governorate, of the analytical descriptive method ، and the Self-Efficacy scale were used, and the results indicated the following:
-The Self-Efficacy level among Musically smart students was high.
-There are no statistically significant differences (α ≥ 0.05)due to the impact of gender in a the total degree .
-There are no statistically significant differences (α=0.05) due to the impact of stage in a the total degree.

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