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Expression of virulence and antimicrobial resistance genes among Escherichia coli clinical isolates from blood and stool samples
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Objective:

As major nosocomial pathogens, Escherichia coli isolates exhibit antibiotic resistance and also express adhesive structures and antibiotic resistance genes. The objective of this study was the comparison of virulence gene expression of extended-spectrum beta-lactamase (ESBL)-producing E. coli between blood and stool samples.

Methods:

In this study, 20 E. coli clinical isolates (10 ESBL-producers including 5 from blood, 5 from stool samples and 10 non-ESBL-producer strains) were included. The existence of fimA, kpsMII and cdt (adhesives and toxin), acr-ab (efflux-encoding) and bla CTX-M1 genes were confirmed by PCR. The quantitative real-time PCR was performed for evaluation of gene expression.

Results:

ESBL-producing E. coli isolates from stool samples could express fimA, kpsMII and cdt genes significantly higher than blood samples, whereas those isolates from blood samples significantly expressed the acr-ab (efflux-encoding) genes. In addition, the bla CTXM1 gene was expressed among isolates from stool samples significantly higher (P = 0.022) than those from blood samples according to the analysis of variance (ANOVA) test. In addition, among non-ESBL-producers, the expression of fimA, kpsMII and cdt genes was significantly lower than ESBL-producing isolates from blood samples, but not significantly different than those from stool samples. Moreover, the expression of acr-ab genes was significantly lower than those from stool samples.

Conclusion:

The results exhibited that the expression of virulence genes among clinical isolates of E. coli is not the same or similar in various conditions or from various clinical origins. Thus determining the profile of gene expression in each of clinical situations can be helpful in tracking the infectious pathogens. ESBL-producing strains possibly have regulatory factors for inducing higher virulence gene expression.

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Publication Date
Wed Sep 26 2018
Journal Name
International Research Journal Of Pharmacy
DENTAL PHARMACOLOGICAL KNOWLEDGE AMONG IRAQI MOTHERS AND ITS IMPACT ON THEIR CHILDREN
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Publication Date
Tue Oct 09 2018
Journal Name
Journal Of The College Of Education For Women
Nutrition Awareness among Middle School Students and its relation to some Variables
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    The aim of the study was to identify the nutritional awareness of middle school students and its relation to some variables of the sample of the research according to the gender variable (male and female), the variable type of family and the variable achievement of the parents.       

      The descriptive approach was adopted and the sample of the study consisted of (795) male and female students who were selected by the random stratified method. The research tool was prepared based on the literature and previous studies. The food awareness measure in its final form was (25) after the data collection was processed using the appropriate statistical met

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Publication Date
Wed Jan 01 2014
Journal Name
Pharmacology & Pharmacy
Gift Acceptance and Its Effect on Prescribing Behavior among Iraqi Specialist Physicians
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Publication Date
Sun Apr 01 2018
Journal Name
Journal Of Educational And Psychological Researches
Forgiveness and its relation to the social intelligence among elementary school students
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The study aims to identify the relationship between forgiveness and social intelligence among elementary school students. The study employed a descriptive analytical approach, whereby a total of (500) elementary school student were selected randomly regarding the variable of gender and economical status. Two scales were prepared: one to measure the forgiveness depending on Albort’s theory that consist of (20) item, and the other to measure the social intelligence according to Tony’s theory which composed of (20) item as well. The result revealed that 6th grade students have interested level of the forgiveness and social intelligence, the girl showed significant differences according to the forgiveness variable, the sample

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Publication Date
Sun Apr 01 2018
Journal Name
Journal Of Educational And Psychological Researches
Educational and psychological adjustment among hostel's students at Omer al-mokhtar University
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Educational and psychological adjustment considered to be one of the effective and serious matters at people dealings and behaviors. Generally, psychological adjustment reflects positively on an individual mental health and their capability to be creative at their field. In contrast to those people who lack this feature. As for educational adjustment, it refers to the compatibility and harmony between an individuals and people around. Thus, these features should be available among students particularly those who stay in students' hostel since they live far from their families. The findings of study revealed that there is Educational and psychological adjustment between male and female. Besides, significant differences were showed

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Publication Date
Sun Mar 19 2023
Journal Name
Journal Of Educational And Psychological Researches
E-Learning and Its Relationship with Academic Passion among Middle School Students
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Abstract

The current research aims to identify the level of E-learning among middle school students, the level of academic passion among middle school students, and the correlation between e-learning and academic passion among middle school students. In order to achieve the objectives of the research, the researcher developed two questionnaires to measure the variables of the study (e-learning and study passion) among students, these two tools were applied to the research sample, which was (380) male and female students in the first and second intermediate classes. The research concluded that there is a relationship between e-learning and academic passion among students.

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Publication Date
Tue Mar 02 2021
Journal Name
Turkish Journal Of Computer And Mathematics Education
Deep understanding skills and their relationship to mathematical modelling among fifth grader
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Abstract: The aim of the current research is to identify (the relationship between deep understanding skills and mathematical modeling among fifth grade students) the research sample consisted of (411) male and female students of the fifth grade of biology distributed over the General Directorates of Education in Baghdad / Al-Rusafa / 2 / and Al-Karkh / 1 /, and two research tools were built: 1- A test of deep understanding skills, consisting of (20) test items and a scale for two skills. 2- The second test consists of (24) test items distributed among (18) essay items and (6) objective items. The psychometric properties of validity, stability, discriminatory strength, and effectiveness of alternatives were verified for the two tests fo

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Publication Date
Thu Nov 08 2018
Journal Name
Iraqi National Journal Of Nursing Specialties
Obesity and Overweight among Students in Arabic Secondary Schools in Erbil City
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Objective(s): The aim of the study was to identify the prevalence of overweight and obesity in adolescence and
to estimate the effect of socio- demographic and health behaviors that predicting obesity in adolescents.
Methodology: A cross-sectional descriptive study was being carried out at three public Arabic secondary
schools in Erbil city from October 1
st 2010 to January 30th 2011. A systematic randomly sample size of 461 students
was selected.
Results: In this study, the age of (46.2%, 122) of males students were ranged between (17- 18.9) years old compared
to females students (74.1%, 146) their age ranged between (15 -16.9) years old. About (3.4%, 9) of males
adolescents having overweight while all female ado

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Publication Date
Tue Jun 23 2015
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
Preparation Gelatin from Chicken Skin and Study its Functional and sensory Properties.: Preparation Gelatin from Chicken Skin and Study its Functional and sensory Properties.
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This study was carrid out to produce animal gelatin from chicken skin. Gelatin was prepared by the chemical method using HCl 2% and extraction at the temperature degree 70, 80, 90 c° and at the period of time 4, 6, 8 hours, calculated the yield, functional and sensory characteristics were measured at. The result also demonstrated that the produced gelatin have good functional properties in solubility, viscosity, gelling capacity, water absorpation, lipid binding, emulsification. viscosity was higher in gelatin prepared at 70 c° and period of extraction 8 hours and reached 1.0846 cp. Gelatin prepared were featured by highe gelling capacity at 1% for all extraction time periods. The produced gelatin was characterized by good sensory qual

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Publication Date
Mon Aug 15 2011
Journal Name
International Journal Of Poultry Science
Effect of Dietary Supplementation with Different Levels of Arginine on Some Blood Traits of Laying Hens
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This experiment was carried out at the Field of Poultry, Department of Animal Resources, College of Agriculture, University of Baghdad, during the period from 1/5/2011 until 5/7/2011 to study the effect of adding arginine to laying hens diet on certain blood traits. A total of 100 Brown Lohmann laying hen chickens, 38 weeks of age, were randomly distributed into four treatment groups, with 25 hens for each treatment. Treatment groups were: T1: bird-fed diet with no additional arginine (control group); T2, T3, and T4: bird-fed diet supplemented with 0.4, 0.7, and 0.9%, respectively. Therefore, the total amounts of arginine in the four treatments (T1, T2, T3, and T4) become 1.1, 1.5, 1.8, and 2.0%, respectively. Results of this experiment rev

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