New evidence on nanotechnology has shown interest in the creation and assessment of nanoparticles for cancer treatment. Worldwide, a wide range of tumor-targeted approaches are being developed to reduce side effects and boost the efficacy of cancer therapy. One strategy that shows promise is the use of metallic nanoparticles to increase the radio sensitization of the cancer cells while reducing or maintaining the normal tissue complication probability during radiation therapy. In this study, atmospheric plasma was created using argon gas to create Au NPs using the plasma jet scheme, and their ability to induce apoptosis as an anticancer mechanism was tested. Aqueous gold tetrachloride salts (HAuCl4·3H2O) ere used to produce gold nanoparticles. For conformations, various techniques were used to explore the characterization of Au NPs, included UV–Vis spectroscopy, X-Ray Diffraction (XRD), Field Emission Scanning Electron Microscopy (FE-SEM),). UV–vis spectroscopy showed a maximum absorption peak located between 520 and 530 nm. The peaks of XRD 2θ are observed at 38.8°, 44.47°, 64.4° and 77.17°. They correspond to the 111, 200, 220 and 311 crystalline levels respectively. The peak intensity (111) at 38.8° diffraction was maximum peak. the image of FESEM showed that the Au NPs which produced are irregularly shaped spheres with sizes ranging between 41-46 nm. The effect of nanoparticles on REF normal cell lines was studied to calculate cytotoxicity and the greatest rate of destruction of REF normal cell lines was 22.667% after incubation time 72 hour after exposure to the combination of irradiated gold nanoparticles-cisplatin with 50 Gray photon X-ray and 1 µg of cisplatin and the minimum was 0.7% after incubation time 24 hour after exposure to the combination of irradiated gold nanoparticles-cisplatin with 0.5 Gray photon x-ray and 0.025 µg of cisplatin. Its possible to enhance chemotherapy treatment by these nanoparticles, In the future these techniques will be possible to use for kill cancer cells, especially after showed low toxicity on normal cells.