A fluorescence microscopy considered as a powerful imaging tool in biology and medicine. In addition to useful signal obtained from fluorescence microscopy, there are some defects in its images such as random variation in brightness, noise that caused by photon detection and some background pixels in the acquired fluorescence microscopic images appear wrongly auto-fluorescence property. All these practical limitations have a negative impact on the correct vision and analysis of the fluorescent microscope users. Our research enters the field of automation of image processing and image analysis using image processing techniques and applying this processing and analysis on one of the very important experiments in biology science. This research is devoted to develop a system based on digital image processing methodology to localize and assess the concentration of saponins accumulation in plant tissues using Fluorescence microscopic image. The proposed system involved preprocessing steps than to make the region of interest more obvious and reflects the saponins accumulation area. Also, the system introduces a simple mathematical way for concentration assessment, and it was justified through the test results. It includes building a system to get microscopic images with best appearance and no defects. It determines the saponins accumulation sites in leaves, rhizomes and shoot apex of Y. gloriosa Variegata and their in vitro cultured tissues (Calli, direct and indirect regenerated shoots and rhizomes/roots). Statistical analysis is performed using a computer to get the mean and median of saponins intensities in each part, and finally perform a comparison between them to determine which part can record the highest intensity level of saponins. The results showed high ability of the system to determine the locations and intensity of saponins in the different parts of the plant. It performs the statistical analysis very quickly. In in vitro culture, it was found that callus treated with Thidiazuron (TDZ) in a combination with benzyl aminopurine (BA) and naphthyl acetic acid (NAA) after 3 weeks of culture had the highest level of saponins accumulation, while the leaves of intact plant recorded the second highest accumulation of saponins.
Aging of asphalt pavements typically occurs through oxidation of the asphalt and evaporation of the lighter maltenes from the binder. The main objective of this study is to evaluate influence of aging on performance of asphalt paving materials.nAsphalt concrete mixtures, were prepared, and subjected to short term aging (STA) procedure which involved heating the loose mixtures in an oven for two aging period of (4 and 8) hours at a temperature of 135 o C. Then it was subject to Long term aging (LTA) procedure using (2 and 5) days aging periods at 85 o C for Marshall compacted specimens. The effect of aging periods on properties of asphalt concrete at optimum asphalt content such as Marshall Properties, indirect tensile strength at 25 o C,
... Show MoreThis research aimed to develop a simulation traffic model for an urban street with heterogeneous traffic capable of analyzing different types of vehicles of static and dynamic characteristics based on trajectory analysis that demonstrated psychophysical driver behavior. The base developed model for urban traffic was performed based on the collected field data for the major urban street in Baghdad city. The parameter; CC1 minimum headway (represented the speed-dependent of the safety distance from stop line that the driver desired) justified in the range from (2.86sec) to (2.17 sec) indicated a good match to reflect the actual traffic behavior for urban traffic streets. A good indication of the convergence between simulat
... Show MoreUsing fiber-reinforced polymer (FRP) could effectively improve the strength and endurance of reinforced concrete (RC) constructions. This study evaluated the flexural behavior of one-way concrete slabs with openings reinforced with glass fiber-reinforced polymers (GFRP) bars. It strengthened using carbon fiber-reinforced polymer (CFRP) sheets around the openings. The experimental program of this study is adopted by casting and testing four one-way concrete slabs with dimensions of (150*750*2650) mm. These slabs are divided into two groups based on whether they were strengthened or un-strengthened. For each group, two different openings (either one rectangular or two square) measured 250*500 mm and 250*250 mm, respective
... Show MoreAbstract. Shock chlorination is a well-known practice in swimming pools and domestic wells. One of the limitations for using this technique in drinking water purification facilities is the difficulty of quickly removing high chlorine concentrations in water distribution systems or production facilities. In order to use this method in the drinking water industry a shock de-chlorination method should be introduced for producing microorganism and biocide free water. De-chlorination using natural stagnant aeration (leaving the water to lose the chlorine naturally) is the safest known method if compared with chemical and charcoaling methods. Unfortunately, stagnant aeration is a slow process. Therefore, developing a process for accelerat
... Show MoreThis paper discusses the method for determining the permeability values of Tertiary Reservoir in Ajeel field (Jeribe, dhiban, Euphrates) units and this study is very important to determine the permeability values that it is needed to detect the economic value of oil in Tertiary Formation. This study based on core data from nine wells and log data from twelve wells. The wells are AJ-1, AJ-4, AJ-6, AJ-7, AJ-10, AJ-12, AJ-13, AJ-14, AJ-15, AJ-22, AJ-25, and AJ-54, but we have chosen three wells (AJ4, AJ6, and AJ10) to study in this paper. Three methods are used for this work and this study indicates that one of the best way of obtaining permeability is the Neural network method because the values of permeability obtained be
... Show MoreLeishmaniasis is one of the neglected parasitic diseases, which belongs to the family Trypanosomatidae. Cutaneous leishmaniasis is endemic in Iraq and the available drugs are of side effect or resistant by the parasite. In this study, cytotoxicity of methotrexate was investigated on the promastigotes proliferation of the Iraqi strain ofL.tropica.The results showed a significant (p ≥ 0.05) difference in growth of treated groupsat all concentration (1000, 500, 250, 125.5, 62.5, 31.25, 15.6) μM, after 24 and 48 hours of follow up, while after 72 hours, significant difference was observed at concentration(1000, 125, 62.5) μM.The IC50 measured after 24and 48 hours and it was 40.366 and 44.452 μM, respectively.The present study showed
... Show MoreObjectives To assess the feasibility and accuracy of a new prototype robotic implant system for the placement of zygomatic implants in edentulous maxillary models. Methods The study was carried out on eight plastic models. Cone beam computed tomographs were captured for each model to plan the positions of zygomatic implants. The hand-eye calibration technique was used to register the dynamic navigation system to the robotic spaces. A total of 16 zygomatic implants were placed, equally distributed between the anterior and the posterior parts of the zygoma. The placement of the implants (ZYGAN®, Southern Implants) was carried out using an active six-jointed robotic arm (UR3e, Universal Robots) guided by the dynamic navigation coordinate tran
... Show MoreThis paper describes flexural behavior of two spans continuous rectangular concrete beams reinforced with mild steel and partially prestressing strands, to evaluate using different prestressing level and prestressing area in continuous prestressed beams at serviceability and ultimate stages. Six continuous concrete beams with 4550 mm length reinforced with mild steel reinforcement and partially prestressed with two prestressing levels of (0.7fpy or 0.55fpy.) of and different amount of 12.7 mm diameter seven wire steel strand were used. Test results showed that the partially prestressed reinforced beams with higher prestressing level exhibited the narrowest crack width, smallest deflection and strain in both steel and concrete at ul
... Show MoreThe nay is one of the important in stvument in Arabic music which is considered one of the oriental instruments used in the oriental music tect, and is also considered one of the basic instruments in Arabic music, It is used in many religious and mundane areas, through its expressive capabilities through which expression and conveyance of feelings to the recipient, despite their importance and role in music, and through the researcher's follow-up to this subject did not find a study on the potential capabilities of the machine. In view of the above, and given the importance of this subject at the researcher, the need arose for research to study (the potential of the flute machine)..
