The experiment was conducted at the plant tissue culture laboratory of the Department of Horticulture and Garden Engineering College of Agricultural Engineering Sciences, University of Baghdad, in order to study the effect of some growth regulators on propagation an stimulation production of volatile oil compounds of rosemary plant Rosmarinus officinlis using two vegetative parts (apical and lateral buds). Factorial experiment was implemented in completely randomized design with twenty replications. The results indicated that culturing the apical meristem on the medium Murashige and Skoog (MS) media with 0.5 mg.l-1 (BA) with 0.1 mg.l-1 of NAA gave the highest response rate of 100%. As for the doubling stage, the levels of BAA and IAA (Indole acetic acid), and their interaction showed a significant effect on the number and length of branches, fresh and dry weight. The treatment of 0.5 mg.liter -1 of BA with 0.0 mg.liter -1 of IAA gave the highest number of branches (5.9 branches.plant-1), and fresh and dry weight (4272and446.2 mg), respectively. Whereas the treatment of 1.5 mg. liter -1 of BA with 0.3 mg. liter -1 of IAA gave the highest length of doubled branches (5.2 cm). The use of BA at a concentration of 0.5 mg.liter-1 was found to increase the active compounds in the volatile oil compared to the MS media free of growth regulator. The best rooting rate of branching was achieved in MS media with complete and half the strength of salts supplied with IBA at a concentration of 0.5 mg.liter-1 or at a concentration of 1 mg. liter -1, where it reached 90%. In addition, the highest number of roots and their lengths in MS media achieved in half of the strength of salts supplied with IBA at a concentration of 0.5 mg.liter-1 reached 5 root. rooted branch-1 and 5.30 cm, respectively. The relative survival rate of the adapted plantlet was 90%