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The thermotolerant Arabian killifish,<i>Aphanius dispar</i>, as a novel infection model for human fungal pathogens
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Abstract<p><italic>Candida albicans</italic>: a fungal pathogen, can cause superficial and fatal infections in humans. An important virulence factor in<italic>C. albicans</italic>dissemination is the transformation from yeast to an invasive hyphal form, which is favoured at human body temperature. Zebrafish, a useful model for studying<italic>C. albicans</italic>infections, cannot survive at 37°C. Arabian killifish,<italic>Aphanius dispar</italic>, an emerging teleost model can tolerate temperatures up to 40 °C for up to 12 days (independent feeding time) allowing for longer analysis compared to zebrafish. This study introduces<italic>A. dispar</italic>as a thermo-relevant and a more accurate reporter of the virulence mechanisms relevant to<italic>C. albicans</italic>as a human pathogen. Using<italic>A. dispar</italic>, we tested virulence at human skin (30 °C), body temperature (37 °C) and a high fever condition (40°C). Infection by<italic>C. albicans</italic>at 37°C and 40°C significantly increased virulence, reduced survival of AKF embryos and formed invasive hyphal network compared to 30 °C. Two mutant strains of<italic>C. albicans. pmr1Δ</italic>(with aberrant cell surface glycans) exhibited reduced virulence at 37°C, whereas<italic>rsr1</italic>Δ (lacking a cell polarity marker) showed less virulence at 30 °C. Additionally, anti-fungal treatment rescued AKF survival in a dose-dependent manner, indicating AKF’s potential for<italic>in vivo</italic>drug testing. Our data indicates the quantitative and qualitative importance of examining virulence traits at physiologically relevant temperatures and demonstrates an equivalence to findings for systemic infection derived in mouse models. The<italic>A. dispar</italic>embryo therefore provides an excellent<italic>in vivo</italic>model system for assessing virulence, drug-testing, and real-time imaging of host-pathogen interactions.</p><sec><title>Significance Statement

The virulence of many pathogens is dependent on host temperature. We demonstrate that theA. disparembryo provides an excellent new thermo-relevant alternative to zebrafish and mouse models, which have limitations in terms of the range of temperatures that can be assessed in real-time. In this study, we have assessedC. albicanstemperature-based virulence, focusing on human body and human skin temperatures (37, 40 and 30 °C, respectively) by examining different genetic backgrounds ofC. albicansstrains. The results indicate differentC. albicansstrains with genetic background show varied virulence depending on temperature indicating importance of examination of virulence mechanisms at physiological temperatures.

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Publication Date
Thu Jan 01 2015
Journal Name
International Journal Of Applied Agricultural Sciences
Effect of Salt Stress on Some Growth Indicators and Cellular Components of Wheat (&amp;lt;i&amp;gt;Triticum aestivum&amp;lt;/i&amp;gt; L.) Callus
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The experiment was conducted to study the effect of sodium chloride (NaCl) at the concentrations of 0.0, 0.5, 1.0 and 1.5% on the callus cells. The Iraq wheat variety was grown in vitro for the purpose of knowing the effect of salt stress on some indicators and cellular components of callus by using a randomized complete design, at the laboratories of tissue culture propagation date palm unit in the College of Agriculture / University of Kufa during the period 2014-2015. Fresh and dry weight, the rate of absolute growth, percentage of dry matter of callus, content of the callus cells of proline, total soluble carbohydrates, sodium and potassium ions, effectiveness of the enzymes catalase and peroxidase study shock salt proteins in callus we

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Publication Date
Sun Dec 30 2012
Journal Name
Al-kindy College Medical Journal
Initial Recognition and Prophecy of Diabetic Nephropathy in Type I Diabetes in a Sample of Iraqi Patients
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Back ground: Diabetic nephropathy is rapidly becoming the leading cause of end-stage renal disease (ESRD). The onset and course of DN can be ameliorated to a very significant degree if intervention institutes at a point very early in the course of the development of this complication.
Objective: The aim of this study was to characterize risk factors associated with nephropathy in type I diabetes and construct a module for early prediction of diabetic nephropathy (DN) by analyzing their risk factors.
Methods: Case control design of 400 patients with type I diabetes mellitus (IDDM), aged 19-45 years. The cases were 200 diabetic patients with overt protein urea while the controls were 200 diabetic patients with no protein urea or micr

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Publication Date
Sat Oct 01 2022
Journal Name
Lark Journal
The role of metaphor in the embodiment of female images (In the novel by Ludmila Ulitskaya "Sincerely Yours Shurik" as model)
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The research deals with metaphors as being one of the primary means used by Lyudmila Ulitskaya when writing the novel " Sincerely Yours Shurik ", to form diverse and multifaceted collective female images of representatives of the classic psychological patterns presented in the work. This research reflects the results of the study related to the work of Lyudmila Yevkinevna Ulitskaya, an actress of modern Russian prose. The novel "The Sincerely to You Shorek" is one of Ludmila Ulitskaya's creations (the year of writing - 2003), which, like her other works, is distinguished by a unique presentation style, rich vocabulary, lexical and semantic diversity, and a special style of writing. writer. The main objective of the research is to look at th

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Publication Date
Mon Oct 09 2023
Journal Name
Nasaq
A Critical Formalistic Reading in Emily Dickinson’s I felt a Funeral, in my Brain and T.S.Eliot’s The Love Song of J. Alfred Prufrock
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A CRITICAL OVERVIEW IN SELECTED POEMS

Publication Date
Wed May 01 2024
Journal Name
Journal Of Studies In Humanities And Educational Sciences
Unlocking the Language of the Heart: A Stylistic Analysis of EmotiveTechniques in Paulo Coelho's By the River Piedra I Sat Down and Wept
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This paper presents an in-depth stylistic analysis of the linguistic artistry andliterary techniques employed by Paulo Coelho in his novel By the River Piedra ISat Down and Wept. A close reading approach guided by stylistics and linguisticsframeworks reveals Coelho's extensive incorporation of imaginative metaphors,vivid imagery, poetic diction, and resonant symbols across the narrative. Analysisspecifically elucidates how Coelho adeptly manipulates various stylistic features toconvey thematic content, shape characterization, and produce aesthetic impacts.Findings provide critical insights into Coelho’s linguistic mastery and contribute toresearch in stylistics and literary linguistics through rigorous examination of anentire contemporary

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Publication Date
Fri Dec 01 2017
Journal Name
Mustansiriya Medical Journal
Assessment of Antimicrobial Sensitivity of Bacterial Pathogens Isolated from Post-Cesarean Surgical Site Infection of Patients in Al-Elwiyah Maternity Hospital
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Publication Date
Wed Jan 01 2020
Journal Name
2nd International Conference On Materials Engineering &amp; Science (iconmeas 2019)
A kinetic model for prodigiosin production by Serratia marcescens as a bio-colorant in bioreactor
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Publication Date
Tue Jan 01 2019
Journal Name
Ssrn Electronic Journal
Silence as a Tactic of Communication in Pragmatics, Novel, and Poetry
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Publication Date
Thu Aug 15 2019
Journal Name
Arab World English Journal For Translation And Literary Studies
Silence as a Tactic of Communication in Pragmatics, Novel, and Poetry
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Publication Date
Tue May 10 2016
Journal Name
Iraqi New Medical Journal January
Which medical specialty do I choose? The answer of Iraqi graduated doctors working in Basra
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OBJECTIVE: To determine the preferred specialties of graduated medical doctors working in Basra, and determine the factors behind their preferences. METHODS: The study was conducted in 38 primary health care centres and seven hospitals in Basra from January-June 2014. A cross-sectional study was adopted with the use of a self-administered questionnaire form. Two hundred ninety six graduated doctors were agreed to participate. Chisquare test and logistic regression were used to test the association between deciding a future speciality and influencing factors. RESULTS: The most preferred specialties were radiology and ultrasound, gynaecology and obstetrics, surgery, internal medicine, dermatology and paediatrics. Clinical specialties were sta

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