Sixty samples from saliva and dental plaque were selected from patients with caries active at ages from 4-65years. 22 isolates belong to Streptococcus mutans. All isolates pronounced adhesion and biofilm formation in various degrees. By using Polymerase Chain Reaction ﴾PCR﴿ Techniques, it was found that these isolates had gtfB encode GtfB with 80 bp, gtfC encode GtfC with 81 bp, and gtfD with 324 bp which explain their potential of biofilm formation.

Cryptosporidiosis is an intestinal protozoan parasitic disease that infects human and animals, caused by apicomplexan parasite belong to the genusof Cryptosporidium. The current study was done to record the infection rate of cryptosporidiosis in human and cattle, and genotype the clinical isolates of Cryptosporidium in Baghdad Province. A total of 265 stool sample were collected (150 from human and 115 from cattle) during the period from December 2016 to the May 2017. Cryptosporidial infection was detected using modified acid fast stain. DNA of the parasite was extracted from oocysts of positive fecal samples and nested PCR method was used for partial 60 kDa glycoprotein (gp60) gene amplification then sequence analysis for selected samples.

Atotal of 551 water samples drinking( five Water Treatment Plans (WTPs) and raw water( from different sites on Tigris river) were collected.According to morphological characteristics and a set of biochemical tests, one hundred twenty eight of Aeromonas spp isolates were obtained In this study The percentages of Aeromonas recovery from river water was 72.52%, from wells water was (35%).Total percentage of positive aeromonas samples of treated water(Filtration &chlorine tank, supply water of WTPs, distribution system, reserviores and other samples not related to WPTs) was 8.8%.Count of Aeromonas in positive aeromonas samples ranging from 1 to 175 cfu 100 ml.
The results showed that generally no significant correlation between presen

Out of 150 different specimens, 67 S. aureus isolate were isolated. However, 16sRNA gene was located only in 60 isolates. Moreover, mecA gene was located in 48 isolates; thereby MRSA covered 80% of all S. aureus isolates. Of considerable interest, pvl gene was detected in only six isolates (10%). Hence, the present work emphasizes the notion suggested that pvl is not an indicative of CA-MRSA.

The resistance of Staphylococcus aureus to ciprofloxacin has complicated the problem of treating staphylococcal associated infections in which MRSA is the causative agent since ciprofloxacin was the drug of choice to treat such infections. Our study investigated the incidence of Ciprofloxacin resistant S. aureus isolates that were also methicillin resistant among Iraqi patients. The obtained bacterial isolates were tested for Ciprofloxacin resistance using agar dilution method and the sequence of gyrA and parC. The results revealed that about 8% of the isolated MRSA strains were Ciprofloxacin resistant and the resistance was due to mutation in gyrA rather than parC.

        One of the most important problems confronts hospitals is the strains emergence  of Enterococcus spp. with multiple resistance to antibiotics, which propel researchers to modify or produce new antibiotics or combination between two antibiotics so that to be more effective against Enterococcus . This study was aimed to susceptibility some of local Enterococcus spp. Isolates with of 21 antibiotic using  disc diffusion method. The results showed absolute resistant 100% toward (Cephalexin , Gentamycin , Amikacin ,Erythromycin and Nalidixic acid), while showed a high sensitivity toward (Vancomycin and Impenem ) at percentage of 92.3% for each . Also highl

The resistance of Staphylococcus aureus to ciprofloxacin has complicated the problem of treating staphylococcal associated infections in which MRSA is the causative agent since ciprofloxacin was the drug of choice to treat such infections. Our study investigated the incidence of Ciprofloxacin resistant S. aureus isolates that were also methicillin resistant among Iraqi patients. The obtained bacterial isolates were tested for Ciprofloxacin resistance using agar dilution method and the sequence of gyrA and parC. The results revealed that about 8% of the isolated MRSA strains were Ciprofloxacin resistant and the resistance was due to mutation in gyrA rather than parC.

        The present study was conducted  to reveal the effect of crude oil  on some  fungal species  isolation from soil in order to evaluate the role of these fungi   in environmental  balance of soil . The results showed a variation in numbers and  percentage of  the fungal  isolates  Aspergillus fumigatus dominated over all   isolates with  a frequency of  (32.47) . In  respect  of  the effect of  different  concentrations of the crude oil,  low concentrations (0.05, 0.1) %  showed no    effect  on radial growth ( mean colony diameter) of the isolated fungi grown &nbs

Background:  Insertion sequence is a short DNA sequence encode for proteins implicated in the transposition activity. Transposase  catalyzes the enzymatic reaction allowing the insertion sequence  to +9*lo2 move. ;qqa;.

Objective: To study the sequencing of transposase gene, tnp, IS1216V of S. aureus isolated from food and then compared with that documented in National Center for Biotechnology Information (NCBI).

Methods: Food samples of animal

Endophytic fungi live inside plants or any part of them without creating any visible pathogenic signs. Endophytic fungi are found within medicinal plants and have shown strong biologic activity, such as anticancer and antioxidant activities, as well as producing extracellular enzymes. In this study, different fungal strains were isolated from the leaves of the medicinal plant Ziziphus spina, including Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Cladosporium sp., Rhizopus sp., and Mucor sp. Extracellular enzymes have been quantified using agar plate-based methods in which fungi were grown in specified growth media to detect the enzymes produced. The results showed that A. niger has the highest ability to produce amy