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Synergy of antibacterial and antioxidant activities from crude extracts and peptides of selected plant mixture
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Abstract<sec><title>Background

A plant mixture containing indigenous Australian plants was examined for synergistic antimicrobial activity using selected test microorganisms. This study aims to investigate antibacterial activities, antioxidant potential and the content of phenolic compounds in aqueous, ethanolic and peptide extracts of plant mixture.

Methods

Well diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays were used to test antibacterial activity against four pathogenic bacteria namelyStaphylococcus aureus, Escherichia coli, Bacillus cereus,andPseudomonas aeruginosa. DPPH (2, 2-diphenyl-1- picrylhydrazyl) and superoxide dismutase (SOD) assays were used to evaluate antioxidant activity. HPLC and gel filtration were used for purification of the peptides. Scanning electron microscope was applied to investigate the mode of attachment of the peptides on target microbial membranes.

Results

Aqueous extraction of the mixture showed no inhibition zones against all the test bacteria. Mean diameter of inhibition zones for ethanol extraction of this mixture attained 8.33 mm, 7.33 mm, and 6.33 mm againstS. aureusat corresponding concentrations of 500, 250 and 125 mg/ml whileE .colishowed inhibition zones of 9.33 mm, 8.00 mm and 6.66 mm at the same concentrations.B. cereusexhibited inhibition zones of 11.33 mm, 10.33 mm and 10.00 mm at concentrations of 500, 250 and 125 mg/ml respectively. The peptide extract demonstrated antibacterial activity againstS. aureus,E. coliandB. cereus. The MIC and MBC values for ethanol extracts were determined at 125 mg/ml concentration againstS. aureusandE. coliandB. cereusvalue was 31.5 mg/ml. MIC and MBC values showed that the peptide extract was significantly effective at low concentration of the Australian plant mixture (APM). Phenolic compounds were detected in hot aqueous and ethanolic extracts of the plant mixture. Hot aqueous, ethanol and peptides extracts also exhibited antioxidant activities.

Conclusions

It was concluded that APM possessed good antibacterial and antioxidant activities following extraction with different solvents. The results suggest that APM provide a new source with antibacterial agents and antioxidant activity for nutraceutical or medical applications.

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Publication Date
Wed Feb 01 2017
Journal Name
Journal Of Economics And Administrative Sciences
Trade relations between Iraq and selected Arab countries for the period 2003 -2013
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Ping message focused on highlighting the fact commodity trading in Iraq, and increased exposure to world merchandise trade imbalance, which dominate Iraq's foreign trade major commodity is oil, and therefore the inability of Iraq to control financial revenue as a result of the fluctuations in the international market, the shortage of commodity products will lead inevitably to the weakness in the ability of the local market to meet the internal demand and due to the lack of flexible production machine For agricultural, industrial and economic sectors are responding to changes in the domestic or external demand which will open the door to merchandise imports to invade these markets, since the adoption of the Iraq oil exports,

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Tue Jun 15 2021
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Consistency and Consistency in Contemporary Iraqi Painting - Selected Models-: حسين شاكر قاسم العيداني
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Sun Oct 22 2023
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Fri Jan 01 2021
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Fri May 15 2026
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Enhancing air pollution exposure tolerance of indoor ornamental plants using glycine and yeast extracts as a bioremediation strategy
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Publication Date
Thu Mar 15 2018
Journal Name
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The Surface Hardness Measurement of Stone and Improved Die Stone After the Addition of a Mixture of Chemical Additives with Different Proportion
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Background: Incorporation of chemical additives has long been a technique used to improve properties of the gypsum products. The purpose of this work was to study the effects of adding a combination of gum Arabic and calcium hydroxide to a type III dental stone and type IV improved die stone with different proportion. The effect on water/powder ratio, and surface hardness was determined. Material and method: Both material stone and die stone were blended with two proportion of additives so that each material was mixed twice but with different proportion of gum Arabic (0.1% and 0.2%) and calcium hydroxide (0.5 % and 0.3%). Data for hardness were subjected to two-way analysis of variance. Results: The results revealed that the chemical additi

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Mon Nov 10 2014
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The Functions of Onomatopoeia in Modern English and Arabic Poetry: A Study in Selected Poems by Lawrence and al-Sayyab
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Mon Jul 06 2026
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High-Performance HDPE/PP Composite Pipes: Morphological, Mechanical, and Antibacterial Insights
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This study developed compatible polymer composites based on a blend of high-density polyethylene (HDPE) and polypropylene (PP), modified with 1% maleic-anhydride-grafted polypropylene (PP-g-MAH) as a compatibilizer. Five formulations were prepared with HDPE/PP ratios of 100/0, 94/6, 89/11, 84/16, and 79/21, each containing 1 wt% (1% by weight) PP-g-MAH. The blends were comprehensively evaluated through morphological, mechanical, thermal, wettability, and bacteriological analyses to determine their suitability for sanitary water-pipe applications. Among all formulations, composite A2 (89% HDPE/10% PP/1% PP-g-MAH) demonstrated the most balanced and superior performance. It achieved a hardness of approximately 70 Shore D and an impact resistan

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Publication Date
Wed Feb 22 2023
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The Adsorption of Some Trace Heavy Metals from Aqueous Solution Using Non Living Biomass of Sub Merged Aquatic Plant Ceratophyllum demersum
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Publication Date
Wed Mar 01 2017
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