The present investigation aims to determine the effects of aflatoxin B1 (AFB1) on biotransformation and antioxidant genes and the protective effects of curcumin, present in turmeric (Curcuma longa) powder (TMP). Specifically, the study included four groups of albino mice were fed for 30 days on diet Group I: Control, Group II: animals fed on the conventional basal diet supplemented with 0.5% food grade TMP that supplied 74 mg/kg total curcuminoids. Group III contained animals reared on conventional basal diet supplemented with 1.0 ppm AFB1 supplied by ground aflatoxin culture material (760 ppm AFB1). Finally, Group IV comprised of albino mice fed with basal diet supplemented with 1.0 ppm AFB1 and 0.5% TMP that supplied 74 mg/kg of the body weight. After treatment, a number of physical parameters were assessed (gain in body weight, average quantity of feed intake and relative liver weight) and it was found that the subjects fed on diet containing curcumin and aflatoxin B1experienced ameliorative effect on the impact of aflatoxin B1 and performed better on growth and liver weight parameters. RNA extracted from the mice liver successfully was subjected to quantitative real time PCR analysis (Q PCR) and the results revealed no high significant difference in the expression of CAT gene between studied groups with probability value ≥0.005. However, at the other hand a decreased in expression statically of SOD, GPx, GST, EH genes was observed while there was an increased Synchronous and consistent expression of CYP1A1 and CYP2H1 genes display in the studied groups in the current study.
Formulations based on nanomaterials have the ability to reduce the consuming of hazardous pesticides and theirimpact on human health and environment. The present study focused on a comparative investigation of histological effects of nanocapule acetamiprid (NACMP) in vivoand commercial parental bulk form of acetamiprid (ACMP) on albino mice. Nanoformulations of pesticides have the potential to improve food productivity without compromising with the ecosystem. In the present study, nanocapsules containing acetamiprid were prepared from two natural macromolecules, alginate and chitosan. The characterization of the nanocapsules were investigated by Dynamic Light Scattering(DLS), T ransmission Electron Microscopy (TEM) and Atomic force
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Eight isolates of methicillin resistance Staphylococcus aureus(MRSA) (SA40,SA32,SA30,SA13,SA10,SA36,SA3 and SA7) with different resistance phenotypes to macrolides , lincosamides and streptogramins Were used to detect theexpression of msrA, msrB, and linA/linA’genesby using real time polymerase chain reaction before and after treatment with antibiotics (erythromycin , clarithromycin , clindamycin and lincomycin) calibrated with triosphosphateisomerase.There highst expression of these genes was after 18 hours. It was an induction in the expression of msrA gene in isolates (SA40,SA32,SA30 and SA13) in presence of erythromycin,however,the isolates showed reduction in expression l
... Show MoreEffect of zinc chloride on the immune functions was studied in male albino mice aged 6-7 weeks. It was administrated orally (1ml) in three concentrations (0.5ppm, 1ppm, 2ppm) for 9 days. The results showed that the first concentration was not effective comparing with control while the second concentration increased the enhancement of immune system and the cell third one killed the mice 6 hours post administration, so we can conclude that the high dose of ZnCl2 could be harmful for all metabolism.
The acute and sub chronic toxicity effects of 25.16 nm intraperitoneally- injected zinc oxide nanoparticles (ZnO NPs) were evaluated. Albino male mice were exposed to three different doses (25, 50 ,and 100 mg/kg ), depending on the value of calculated LD50, for 2 and 4 weeks . Considerable changes in organ indexes were shown with a good relevance to the illustrated histopathological effects which ranged from multiple hemorrhagic foci in liver, mild swelling and dilatation in kidney tubules, thickening of intestinal villi, moderate interstitial pneumonia, especially with the high dose , and sever necrosis of seminiferous tubules in testes of all studied groups. Significant changes in both hematological and biochemical parameters as well a
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The inorganic phosphate content and antioxidant activity of fresh fruit juices and canned fruit juices commonly consumed in Bahrain were compared. The fruits considered in this study were kiwi, guava, black grape, strawberry, apple, and pineapple. The inorganic phosphate content of the juices was determined by a colorimetric method using a UV/VIS spectrophotometer. Among the fresh juices, the highest inorganic phosphate content was measured for black grape juice (17.330 ±0.068 mg/L), and among the canned juices, the highest inorganic phosphate was measured for black grape canned juice too (16.020 ±0.141 mg/L, brand 3). The antioxidant activity was determined in-vitro by measuring the percentage of 2,2-diphenyl-1-picrylhydrazyl
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Background: Iraqis were exposed to wars, widespread violence and civil war. Post-traumatic stress disorder develops after exposure to trauma and violence. It has a negative effect on academic achievement of the students.
Objective: This report was carried out to study the effect of exposure to violence on academic achievement of youths in Iraq.
Methods: A total of 319 university students from Baghdad were included in the study. Their age ranged between 18 and 24 years with male to female ratio of 0.6:1. A questionnaire was filled for each participant. Requested data were demographic information, data on school achievement and Harvard Trauma questionnaire (exposure to war trauma, posttraumatic stress disorder “PTSD”). Chi square
The gene expression of the most important structural genes ica A and D of biofilm, sarA, and sigB regulatory genes of some methicillin-resistant Staphylococcus aureus (MRSA) isolates were examined using the real-time polymerase chain reaction after 24 hours of growth. The results revealed that the isolates with strong biofilm production had the highest gene expression of the structural icaA and D genes. Whereas the isolates that showed moderate and weak biofilm production, recorded the lowest gene expression. The results of the regulatory genes sarA, and sigB fluctuated among all MRSA isolates. Isolate No. 64 recorded the highest gene expression
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Background: Breast cancer is the culmination of a multi-step process that occurs over a period of several years or decades and as a cause of death, is a salient "free radical" disease. Aim: The present study aims on investigating the possible protective role of antioxidant drugs (vitamins E and C) to cardiac cells against the oxidative stress induced damage during doxorubicin chemotherapy in patients with breast cancer.
Patients and methods: Thirty two patients with different stages of breast carcinoma attending to Baghdad Teaching Hospital and ten healthy control subjects with age range between (29-61) years, mean (43.6±1.37) were included in this study. The patients were randomized into 3 groups, they
This study was aimed to assess the impact of vermicompost, glutathione, and their interaction on beetroot (Beta vulgaris L.) growth, yield, and antioxidant traits. The experiment carried out at vegetable field of the College of Agricultural Engineering Sciences - University of Baghdad during fall season 2019. The experiment was conducted using factorial arrangement within Randomized Complete Block Design with two factors and three replicates (3X3X3). Applying vermicompost before cultivation represented the first factor (0, 15, 30 ton.ha-1), which symbolized (V0, V1, V2). Glutathione (0, 75, 150 mg.L-1) which symbolized (G0, G1, G2) represented the second factor. Results showed the superiority of secondary interaction treatment V2G2
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