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Effects of Tribuls terrestris (Quttub) and clomiphene citrate on ovaries of female mice; Histological and histochemical study

Abstract: Background: Tribuls terrestris increases levels of various hormones in steroid family including testosterone, DEHA, and estrogen and for this reason improves sport performance, fertility in men and women, sexual function in men and women. There is, at present, lack of scientific confirmation of these supposed benefits. Therefore, this study aims to investigate the possible effect(s) of Tribuls terrestris on the mouse ovarian morphology and function, alone and in combination with other ovulation modulator agent (clomiphene citrate). Materials & Methods: A total of 49 sexually mature healthy Norway albino female mice were used in this study; 25 for pilot study and 24 for the experimental study. Experimental animals were divided into 4 groups, each contained 6 animals. The 1st group was given Tribuls terrestris, the 2nd group was given clomiphene citrate, the 3rd group was given the two agents together, and the 4th group was control. Treatment was given daily for 10 days via orogastric intubation. Histological, histochemical, cytological and morphometrical studies were carried out. Results: demonstrated that Tribuls terrestris alone causes an increase in the number and size of the mature follicles, with no significant change in the total follicular number, with obvious progesterone and some estrogen effects on the otherwise normal vaginal smears. On the other hand, clomiphene alone revealed no mature follicles and no corpora lutea, with strong estrogen-like effect and minimal progesterone effects on the vaginal smears. When Tribuls terrestris and clomiphene were given together, mature follicles and corpora lutea appeared in relatively large numbers, with estrogen and progesterone effects on the vaginal cytology. Conclusion: Tribuls terrestris can stimulate ovulation when given alone, and oppose the anti-ovulatory effect of clomiphene and resume ovulation when given in combination with it. Keywords: Tribuls Terrestris (Quttub), Clomiphene Citrate, Mice Female Ovaries

Publication Date
Tue Nov 26 2024
Journal Name
Journal Of Baghdad College Of Dentistry
Histological evaluation of local application of collagen I and /or vascular endothelial growth factor (An experimental study in rats)

Background: The study was designed to evaluate the effect of local application of exogenous VEGF/collagen I separately and as a combination in socket healing. Sixty male Albino Wistar rats were subjected for a surgical tooth extraction of upper 1st molar of both sides (right side was considered as experimental site, while left be the control one, treated with 1µL of normal saline). The rats were scarified at 3, 7, 14, 28 days post extraction. Socket healing was histologically examined with immunohistochemistochemical localization of ALP&FGF2. Materials and Method: Sixty male Albino Wistar rats were subjected for a surgical tooth extraction of upper 1stmolar of both sides (right side was considered as experimental site, while left be the

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Publication Date
Tue Nov 26 2024
Journal Name
Journal Of Baghdad College Of Dentistry
Histological evaluation of the effect of topical application of Curcumin powder and essential oil on skin wound healing

Background: Herbal medicine can be called one of the branches of medicine in various forms. Turmericcurcumin has proved its efficiencies a coloring, flavoring agent and has been traditionally used in medicine, exhibiting remarkable anti-inflammatory and antioxidant properties. The varied biological properties of curcumin and lack of toxicity even when administered at higher doses makes it attractive to explore its use in various disorders like diseases of skin. It is good potential agent for wound healing. Materials and methods: Sixty four new Zealand rabbits were used in this study ,they were divided into four groups,each group was subdivided as follows:Experimental groups(8 rabbits) right facial side of animals for essential oil applicati

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Publication Date
Sat Jun 25 2022
Journal Name
International Journal Of Drug Delivery Technology
Study of Histological Structure of Mesencephalon in White Cheeked Bulbul (Pycnonotus leucotis)

The histological structure of Pycnonotus leucotis was investigated to fill the dearth of information on the histology of mid-brain from available literature and help understand its brain. The brain is wide and short and its length 1.5 cm, and it consists of three regions. The middle region is the mesencephalon. The mesencephalon was divided into optic tectum and tegmentum. The optic tectum consists of six main layers, while the tegmentum contains nuclei of cranial nerves.

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Publication Date
Mon Apr 10 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Histological Study of the Digestive Tract of Liza abu (Heckel) 1-Esophagus

  The study includes histological structure of  digestive tract of  Liza abu , Anatomical results found that the digestive tract occupied the largest part of  abdominal cavity ,  the mean gut length was (45.5) cm and relative gut length was (2.9) .  esophagus was a muscular tube length (1.5) cm , esophagus wall containing longitudinal  straight unbranched folds numbering (9) . Histological study  results found that the esophagus composed of four tunica : mucosa , submucosa , muscularis and serosa . The results found that the mucosa consists of three layers : epithelium , lamina propria and muscularis mucosa . The epithelial layer was stratified squamous epithelium thickness (139.1) µ containing

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Publication Date
Thu Dec 29 2016
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Histological Study of the Digestive Tract of Liza abu (Heckel) II Stomach

  The study current included histological structure of stomach of Liza abu , anatomical results found that stomach represent the expander non-convoluted part of gastrointestinal tract it is located between the end of the esophagus and the beginning of the intestine , the average length of stomach was (1.5) cm , composed of two parts : cardiac stomach which is represent the anterior part and be thin- walled , the other part was pyloric stomach or gizzard and the walls were thick . The internal surface of cardiac and pyloric stomach contain longitudinal folds numbering (7), folds branching to finger shape structures extend inside the lumen . Histological study showed that stomach wall composed of three main histological layers : mucos

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Publication Date
Sun Jun 11 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Histological Study of the Digestive Tract of Liza abu (Heckel) 3- Intestine

 The study includes histological structure of  pyloric caeca , intestine and rectum of Liza abu , the results show that the pyloric caeca are non-branched fingerlings structures numbering (4) and length rate was (0.5) cm , while the intestine of Liza abu was long narrow tube composed of a number of laps , the anterior part of intestine was wider in diameter than the posterior part , the length of intestine was (32) cm , the rectum was straight tube extends from the end of posterior part of intestine to the anus.         The wall of pyloric caeca , intestine and rectum consists of main layers : mucosa , submucosa , muscularis and serosa , lining epithelium of  pyloric caeca , intestin

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Publication Date
Sun Jun 03 2018
Journal Name
Baghdad Science Journal
Morphological and Histological Study of the Forebrain (Cerebrum) in a Wild Bird Species (Columba livia domestica) (Gmelin, 1789)

The present study deals with the morphological and histological aspects of the forebrain(Cerebrum) in the Columba livia domestica (Gmelin, 1789) to identify the histoarchitecture of its layers. This bird' has a large head found as perpendicular to the longitudinal axis. The morphological results reveal that for brain (Cerebrum) pear shaped, its outer surface is smooth without folds or deep grooves. Cerebrum is made up of two regions, the Pallium and the Subpallium. The Cerebral cortex includes four layers of hyperpallium (Wulst) , Dorsolateral corticoid area (CDL), Hippocampus, Piriform cortex. The internal cortex of cerebrum consists of Dorsal Ventricle ridge which includes the mesopallium, nidopallium, and archospallium. All these reg

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Publication Date
Sun Jun 03 2012
Journal Name
Baghdad Science Journal
Study the effect of polyphenols extracted from Iraqi grape seeds on glucose , MDA levels and GST activity in streptozotocin (STZ ) induced diabetic mice.

1-Objective:- Polyphenols are biochemical compounds with antioxidant activity against differences diseases related to Lipid peroxidation such as diabetes mellitus. Polyphenols distributed widely in medical plants, the aim of the study is to extract and analyze some polyphenolic compounds from grape seeds and examine their effects on (STZ) induced diabetic mice. 2-Methods:- In the present study , a group of polyphenols has been extracted from Iraq

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Publication Date
Sun Jun 09 2019
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
Possible Amelioration of the Severity of Nutritional Steatohepatitis by Guggulsterone in Mice: guggulsterone and steatohepatitis in mice

Non-alcoholic fatty liver disease (NAFLD) has become one of the most common chronic liver diseases worldwide, which characterized by steatosis, inflammation, and fibrosis. The aim of this designed study is to evaluate the ability of guggulsterone to prevent high fat diet induced steatohepatitis in mice. Five groups of male mice were selected and treated as the following: group I, mice had free access to standard commercial diet and considered as control group, group II, mice were fed a specially formulated high-fat diet for 12 weeks to induce non-alcoholic liver disease, while groups III, IV and V the mice were administered high fat diet containing guggulsterone at 500, 1000 and 2000 ppm concentration respectively for 12 weeks. Maintaini

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Publication Date
Tue Jan 02 2018
Journal Name
Journal Of Educational And Psychological Researches
Family violence, its causes and effects on society (women and children)

Summary of the research Family violence, its causes and effects on society (women and children). Search Goal: The current research aims to identify: 1. Causes of family and social violence (for children and women). 2. How to process. research importance : Domestic violence is a major and devastating problem that has a negative and direct impact on children in particular and women or mothers in general. The problem of domestic violence is linked to many factors and individual, social, economic, psychological and environmental variables, which makes one theory that claims to be inaccurate. It is difficult to understand the nature of family violence without linking it to some of the concepts related to it. The negative effects that may lead

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