Hydatiosis is a widespread chronic zoonotic parasitic disease caused by helminthic larval stage of tapeworm Echinococcus granulosus.. In last year's many methods are used to activate and modulate the response of immune system of the host in order to evade the invasion of the parasite. The present investigation was aimed to find out the effect of attenuated protoscolices on the viability of lymphocytes which play very important role in the immune response against many parasites and the reflect of this attenuation on hydatid cyst protoscolices infectivity in vivo. Four groups of white male BALB/c mice were experimentally infected with helium-neon laser attenuated protoscolices that exposed to helium –neon laser for (10,30,50,100) minutes respectively. The results showed increase in lymphocyte viability (p<0.01), especially among mice that exposed to laser for 50 minutes (77.00±1.42) in comparison to control group(50.66±3.23),this increase in lymphocyte viability causes statistically (p<0.01) decrease in protoscolices infectivity (both cyst numbers and diameters)in comparison with positive control group which cause decrease in lymphocyte viability.
Effect of attenuated protoscolices of hydatid cyst worm
( Echinococcus granulosus) on lymphocytes viability in vivo
Echinococcus granulosus
Protoscolices. Attenuation. Lymphocytes
Viability
Laser
Publication Date
Mon Aug 01 2011
Journal Name
Journal Of Engineering
PHOTO DYNAMIC THERAPY (PDT) WITH BIOLOGICAL TISSUES USING ND:GLASS LASER
Photo Dynamic Therapy (PDT) technique
a pulsed Nd:glass Laser
Z-necrosis
Dyes with human tissues
Photosensitizers.
A Photo Dynamic Therapy (PDT) is a technique which is used with Laser to treat many of cancer
tissues. This paper deals with the relatively new therapeutic technique (PDT) with pulsed Nd:glass Laser
which was applied to human soft tissues (Ovary and Kidney tissues), and to the hard tissues (freshly
extracted human teeth), with power density of 280 watt/mm2 and exposure time 330 usec. Different
dyes (Blue, methylene, eosin, and orange) were applied to the area before irradiation to study the effect
of the pigments on the laser interaction with biological tissues. The zone of treatment (Z-necrosis) with
aid of MATLAB was determined. The relationship of zone of treatment with exposure time,
accumulated damage and fracti
