Background: Human leukocyte antigen-G (HLA-G)and Toll-like receptor-9 (TLR-9)play a role in the regulation of autoimmune diseases and inflammatory processes. Aim of the study: To detect the HLA-G + 3142G > C gene polymorphism that associated with the susceptibility to SLE patients and associated with Hepatitis B infection and TLR-9 serum level. Patients and methods: This study was done on 75 SLE patients and 75 healthy control groups. Genotyping of HLA-G + 3142G > C were detected by PCR and PCR-RFLP methods. In addition to the estimation of Hepatitis B surface (HBs)antigen status by immunochromatography technique and TLR-9 serum level by ELISA technique. Results: The HLA-G + 3142G > C gene polymorphism between the SLE patients and controls in CC, CG and GG genotyping (5.33% vs. 45.34%; OR = 0.07; P = 1.0 × 10−8, 21.33% vs. 37.34%; OR = 0.46; P = 0.048, 73.33% vs. 17.33%, OR = 47.45; P = 4.5 × 10−12 respectively). In addition, there was a significant increase frequency of G allele in SLE patients compared to controls (0.84 vs. 0.36). While the C allele showed a significantly decreased frequency in patients compared to controls (0.16 vs. 0.64). The seropositive status of HBs antigen showed no significant difference between the SLE patients' group and controls (OR = 7.3, 95%CI = 0.38–140.81). While the mean of TLR-9 serum level was significantly increased in SLE patients' group compared to the control group (399.9 ± 66.7 pg/ml vs. 122.2 ± 28.5 pg/ml; p ≤ 0.05). Conclusion: The HLA-G + 3142G > C homozygous genotype GG and G allele were more associated with SLE patients. The HBs antigen showed no significant association with SLE patients. While, TLR-9 serum level showed a significant association with SLE development.
BACKGROUND: Carcinoma of urinary bladder is one of the most common malignancies worldwide and constitutes a major health problem. Multiple risk factors are associated with this tumor and its prognosis will depend on different clinicopathological parameters. Over expression of P53 protein and mutant Rb gene is associated with more aggressive clinical and histopathological features of the tumor such as advanced stage and higher grade. AIM: The immunohistochemical expression of Rb gene and P53 gene will be assessed through their protein products in transitional cell carcinoma (TCC) of the urinary bladder and then will be correlated with other well-known risk factors and prognostic parameters of bladder TCC, such as grading, tumor size, smoking
... Show MoreBackground: Irritable bowel syndrome (IBS) is a functional bowel syndrome with discomfort and changed bowel habits in the absence of obvious structural abnormalities. It is more common among adolescents and young age groups. Aim: Aims of the study is to identify the prevalence of Irritable Bowel Syndrome among medical students of AL- Kindy College of medicine, and to Exploring the effect on IBS prevalence of general demographic form to observe the association between anxiety and IBS. Data were collected on a sample of University of Baghdad, Al-Kindy College of Medicine students in Baghdad, Iraq. The study was conducted over a period for 6 months From December 2020 to June 2021. This study enrolled 250 students who were selected rand
... Show MoreABSTRACT The present study was conducted to determine the mode of presentation of hypothyroidism in pediatric patients and the effects of timing of diagnosis and therapy on the patient’s outcome. The study involved review and evaluation of the medical records of 41 registered patients in the Endocrine clinic of Children Welfare Teaching Hospital in Baghdad during the period from January 1991 to July 2007. Forty one patients included in this study. Twenty four (58.5%) were males and17 (41.4%) were females with a male to female ratio of 1.4:1, their age range is 40 days to12.3 years. The majority of the studied patients were infants 19(47%). The most commonly observed presenting features were growth retardation and short stature. The best t
... Show MoreKE Sharquie, HA Hassan, AA Noaimi, IRAQI JOURNAL OF COMMUNITY MEDICINE, 2010
A chemical study was carried out to evaluate the efficiency of the liver enzyme concertation and uric acid level and its antagonists in the serum of the professors exposed to chemicals in the laboratories of the University of Samarra and their comparison with the healthy people. The research included 25 samples of the exposed professors and 20 samples as a group of officers.
The results of the current study showed a significant increase in the level of AST, ALT in the serum of professors exposed to chemicals compared to healthy people. The results showed no significant increase in
... Show MoreAN Adil A, F Basman M, 2009
Rheumatoid arthritis (RA) is a systematic autoimmune disorder with chronic inflammation changes of unknown etiology. Various synovial inflammatory and proliferative alterations may contribute to the cartilaginous tissues and invasive bony tissues, leading to destructive joints and malformed bones. This disease is mostly due to infective microorganisms or genetic susceptibility causing immune system disturbances through triggering both T-cells and B-cells. Furthermore, different immune cells may secret cytokines, which are responsible for some RA pathogenesis activity. From ninety individuals, serum sample was collected; thirty of them were normal and sixty cases were patients with RA attended a privet medical clin
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