Preferred Language
Articles
/
kRcMGo8BVTCNdQwCV1tL
Cloning and expression of a lipase gene from Pseudomonas aeruginosa into E.coli
...Show More Authors

Fifteen local isolates of Pseudomonas were obtained from several sources such as soil, water and some high-fat foods (Meat, olives, coconuts, etc.). The ability of isolates to produce lipase was measured by the size of clear zone on Tween 20 solid medium and by measuring the enzymatic activity and specific activity. Isolate M3 (as named in this study) was found to be the most efficient for the production of the lipase with enzymatic activity reached 56.6 U/ml and specific activity of 305.94 U/mg. This isolate was identified through genetic analysis of the 16S rRNA gene. and it was shown that the isolate M3 belongs to Pseudomonas aeruginosa with 99% similarity. The DNA of isolate M3 was extracted and lipase gene was amplified through PCR technique, then purified and cloned into E.coli DH5α cells first using pTG19-T plasmid, and expressed in E.coli Bl21 with expression vector pet-28a. The activity of lipase from transformed E.coli Bl21 was 196.6 U/ml and the specific activity 618.2 U/mg.

Scopus
Publication Date
Thu Jan 30 2014
Journal Name
Al-kindy College Medical Journal
Serological and histopathological study of thyroid diseases, prospective study
...Show More Authors

Background: Accurate detection of thyroid autoantibodies by enzyme linked immunosorbant assay technique namely thyroglobulin antibody, thyroid peroxides antibody is crucial in the differentiation of autoimmune thyroid disorders from other form of thyroid diseases.
Objective: Evaluation of the detection of thyroglobulin antibody and thyroid peroxides antibody in different thyroid diseases using enzyme linked immunosorbant assay technique.
Methods: - Seventy-five patients admitted to Surgical Units of Baghdad Medical City Hospital for the period between "October 2010 to June 2011" they were waiting to do thyroidectomy. They were chosen nonselectively for serological evaluation of above autoantibodies , and correlation of the results

... Show More
View Publication Preview PDF
Publication Date
Sun Apr 30 2023
Journal Name
Iraqi Journal Of Science
Dynamics and Chaotic of Polynomials on Quasi Banach Spaces
...Show More Authors

In the present paper, the concepts of a quasi-metric space, quasi-Banach space
have been introduced. We prove some facts which are defined on these spaces and
define some polynomials on quasi-Banach spaces and studied their dynamics, such
as, quasi cyclic and quasi hypercyclic. We show the existence of quasi chaotic in the
sense of Devaney (quasi D-chaotic) polynomials on quasi Banach space of qsummable
sequences lq , 0<q<1 such polynomials P is defined by P((xi)i)=(p(xi+m))i
where p:CC, p(0) = 0. In general we also prove that P is quasi chaotic in the sense
of Auslander and Yorke (quasi AY-chaotic) if and only if 0 belong to the Julia set of
p, mN. And then we prove that if the above polynomial P o

... Show More
View Publication Preview PDF
Publication Date
Sat Sep 01 2007
Journal Name
Al-khwarizmi Engineering Journal
Flutter Speed Limits of Cantilever Rectangular and Tapered Plates
...Show More Authors

The aerodynamic and elastic forces may cause an oscillation of the structure such as the high frequency of the airfoil surfaces and the dynamic instability occurring in an aircraft in flight and failure may occur at a speed called flutter speed. In this work, analytical and numerical investigations of flutter limits of thin plates have been carried out. The flutter speed of rectangular plates were obtained and compared with some published results. Different design parameters were investigated such as aspect ratio, thickness and their effects on flutter velocity. It was found that the structural mode shape plays an important role in the determination of the flutter speed and the coupling between the bending and torsional mode is the main

... Show More
View Publication Preview PDF
Publication Date
Mon May 31 2021
Journal Name
Iraqi Journal Of Science
Stability and Anti-Chaos Control of Discrete Quadratic Maps
...Show More Authors

A dynamical system describes the consequence of the current state of an event or particle in future. The models expressed by functions in the dynamical systems are more often deterministic, but these functions might also be stochastic in some cases. The prediction of the system's behavior in future is studied with the analytical solution of the implicit relations (Differential, Difference equations) and simulations. A discrete-time first order system of equations with quadratic nonlinearity is considered for study in this work. Classical approach of stability analysis using Jury's condition is employed to analyze the system's stability. The chaotic nature of the dynamical system is illustrated by the bifurcation theory. The enhancement o

... Show More
View Publication Preview PDF
Scopus (1)
Crossref (1)
Scopus Crossref
Publication Date
Tue Dec 01 2015
Journal Name
Bulletin Of The Iraq Natural History Museum (p-issn: 1017-8678 , E-issn: 2311-9799)
UPDATED LIST OF AMPHIBIANS AND REPTILES IN IRAQ 2014
...Show More Authors

    The present work provides a list of all amphibians and reptiles recorded from Iraq up to 2014. It includes 115 species (105 species of reptiles and 10 species of amphibians) dating back to 25 families (20 families of reptiles and 5 families of amphibians). Conservation status of each species was mentioned.

View Publication Preview PDF
Publication Date
Thu Dec 29 2016
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Anatomical and Palynological study of Ricinus communisL.from Euphorbiaceaein Iraq
...Show More Authors

  The anatomical study showed the epidermis leaves of Ricinus communis L.glabrous and withparacytic pattern, also the midrib of leavescomposedfrom a pyramidal shapearea with a circular tip like ascamel humpfrom the upper side consisting of supported collenchyma tissue, and the cross section of leaf petiole formed as an ovate shape as for the cross section of stem was a circular, also observed many druces crystaland aleuron grains that are spread in the cortex area. Pollen grain appeared under a scanning electron microscope that tricolporate, either the surface pattern was reticulate and the shape of pollen grain prolate spheroidal to subprolate

View Publication Preview PDF
Publication Date
Sun Feb 01 2015
Journal Name
Journal Of Engineering
Desulfurization of Diesel Fuel by Oxidation and Solvent Extraction
...Show More Authors

This research presents a study in ultra-desulfurization of diesel fuel produced from conventional hydro desulfurization process, using oxidation and solvent extraction techniques. Dibenzothiophene (DBT) was the organosulfur compound that had been detected in sulfur removal. The oxidation process used hydrogen peroxide as an oxidant and acetic acid as homogeneous catalyst . The solvent extraction process used acetonitrile (ACN) and N-methyl – 2 - pyrrolidone (NMP) as  extractants . Also the effect of five parameters (stirring speed :150 , 250 , 350 , and 450) rpm, temperature (30 , 40 , 45 , and 50) oC, oxidant/simulated diesel fuel ratio (0.5 , 0.75 , 1 , and 1.5) , catalyst/oxidant ratio(0.125,0.25,0.5

... Show More
View Publication Preview PDF
Publication Date
Thu Dec 29 2016
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Toxicological and Physiological Effects of DDT on Caenorhabditis elegans
...Show More Authors

Toxicity assays were used in this study to test how DDT affects lethality and brood size of Caenorhabditis elegans (C.elegans) by exposing them to various concentrations of this agent. These nematodes have provided a very informative system that was utilized to study the behavioural and physiological processes. The results showed that DDT affected the lethality in a dose-dependent manner, but 100% kill was not achieved with concentration tested. Whereas, sodium azide, positive control does have an effect on C. elegans and significantly inhibiting lethality (LC50 0.01mM).  Similarly, DDT led to a pronounced effect in brood size of C.elegans compared to the mean brood size recorded for the control (0.1% DMSO). Sodium azide results sho

... Show More
View Publication Preview PDF
Publication Date
Fri Feb 01 2019
Journal Name
Journal Of The College Of Education For Women
Words worth and the Intention of AL. Sayyab Discourse
...Show More Authors

Words worth and the Intention of AL. Sayyab Discourse

View Publication Preview PDF
Publication Date
Sun Dec 10 2017
Journal Name
Al-academy
Form and function in the design of intellectual toys
...Show More Authors

Playing is the only common language among children in every place and time, to the fact that what distinguishes the children is their quest to play and fun way innate, not acquired, which is expressed with the child really is one of the rights. And play a key entrance to the growth of the child mentally, intellectually and cognitively and not for the child emotionally and socially just. In the play the child begins to identify and classify objects and learn concepts and circulate among themselves on a linguistic basis. Activity here and play a major role in leading the linguistic development of the child in the composition has the communication skills.

The Games (especially intellectual ones) Whatever the cause of the development

... Show More
View Publication Preview PDF
Crossref