The study aims to measure the level of academic stress in the e-learning environment in three areas, students and their dealing with classmates, dealing with the professor and technical skills, and the nature and content of the curriculum among graduate students in the College of Education at King Khalid University during COVID-19 pandemic. This study was descriptive in nature (survey, comparative). The sample consisted of (512) male and female graduate students in the master's and doctoral programs. The Academic Stress Scale in the E-learning Environment designed by Amer (2021) was used. The results indicated a high level of academic stress among graduate students in the e-learning environment. The study also found that there were statistically significant differences in the level of these stresses based on gender with female students reporting higher score means than male students. Moreover, results revealed that there were statistically significant differences in the level of these stresses between masters and doctoral students in favor of master's students. However, there were no statistically significant differences found based on the variable of specialization.