Background: Enterococcus faecalis is a causative agent for urinary tract infections (UTIs) in Iraq and worldwide, even though it is a commensal bacterium in human and animal intestines. It can cause different illnesses due to its ability to produce various virulence factors. A pore-forming toxin (cytolysin) is the most virulence factor in this bacterium. Objective: This study aims to molecularly investigate the frequency of cytolysin toxin among E. faecalis isolated from UTIs. Methods: A hundred and eighty urine specimens were collected from females diagnosed with UTIs. Traditional laboratory and molecular methods were used for bacterial identification and toxin detection using a modified DNA extraction method. Results: The findings reveal

Select 30 isolate from Bacillus to detect the ability to produce pullulanase enzyme in liquid and solid state fermentation, and use the isolate Bacillus licheniformis (Bs18) because the highest production of enzyme, the optimum condition for the production of enzyme by liquid state fermentation (LSF) in growen with: media contains starch + pullulan as a carbon source, peptone as a nitrogen source, inoculums size 2 ml, and incubated at 40 C° with pH 7 for 48 hrs. In addition pullulanase production by solid state fermentation (SSF) was investigated using isolated Bacillus licheniformis (Bs18). Optimization of process parameters were carried out ,the optimum solid substrate , Temperature , pH , incubation period , inoculation size , hydrat

In this work, microbubble dispersed air flotation technique was applied for cadmium ions removal from wastewater aqueous solution. Experiments parameters such as pH (3, 4, 5, and 6), initial Cd(II) ions concentration (40, 80, and 120 mg/l)  contact time( 2, 5, 10 , 15, and 20min), and surfactant (10, 20and 40mg/l) were studied in order to optimize the best conditions .The experimental results indicate that microbubbles were quite effective in removing cadmium ions and the anionic surfactant SDS was found to be more efficient than cationic CTAB in flotation process. 92.3% maximum removal efficiency achieved through 15min at pH 5, SDS surfactant concentration 20mg/l, flow rate250 cm³/min and at 40mg/l Cd(II) ions initial co

     A mineralogical study using X-ray diffraction supported by scanning electron microscopic examination on the Paleocene- Eocene Kolosh and Gercus formations from northern Iraq is conducted to show the distribution of clay minerals and their paleoenvironmental implications. Smectite palygorskite, kaolinite, illite, and chlorite are commonly present in varying proportions within the Kolosh and Gercus formations. Association of smectite and chlorite in the claystone of the Paleocene Kolosh Formation refers to marine environment of this formation, whereas development of palygorskite fibers from smectite precursor may relate to post-depositional diagenesis. In addition, the abundance of illite and kaolinite in the Eocen

The aim of this study is the relationship between blood group and group A Beta-Hemolytic Streptococci, Ninety patients with tonsillitis were included in this Study from both genders and different age group for blood group study. They were attended at Ramadi Teaching Hospital during the period extended from January 2011 to June 2011. Their ages range from 3-35years.The control group included 20apparently healthy persons, while patient divided into, chronic and recurrent acute tonsillitis. Diagnostic tools were Bacteriological methods (Bacitracin method to identify and count Streptococcus pyogenes group A beta hemolytic streptococci (GABHS ).Results of blood grouping showed a reduced frequency of blood group AB- with tonsillitis and increa

Effect of drought stress induced by mannitol on secondary products in callus of Golden Sunrise cherry tomatoes were studied in vitro. Seeds were irradiated with gamma at the doses (0, 20, or 40 Gy), and germinated on Murashige and Skoog, (MS) basal medium. Callus were initiated from cotyledon leaf explants using a combination of (2.0 kin + 2.0 IAA mg. l^-1) then transferred into MS medium supplemented with mannitol at concentrations of (0, 40, and 60 g.l^-1). Vitamin C(vit C), enzyme activity, Ascorbate peroxidase (Apx), and Amylase  enzymes, Proline amino acid and β- carotene were quantified by high-performance Performance Liquid Chromatography (HPLC). Results revealed that, highest contents of (Apx) 128.16 µg. m

Out of 150 clinical samples, 50 isolates of Klebsiella pneumoniae were identified according to morphological and biochemical properties. These isolates were collected from different clinical samples, including 15 (30%) urine, 12 (24%) blood, 9 (18%) sputum, 9 (18%) wound, and 5 (10%) burn. The minimum inhibitory concentrations (MICs) assay revealed that 25 (50%) of isolates were resistant to gentamicin (≥16µg/ml), 22 (44%) of isolates were resistant to amikacin (≥64 µg/ml), 21 (42%) of isolates were resistant to ertapenem (≥8 µg/ml), 18 (36%) of isolates were resistant to imipenem (4- ≥16µg/ml), 43 (86%) of isolates were resistant to ceftriaxone (4- ≥64 µg/ml), 42 (84%) of isolates were resistant to ceftazidime (1

     This paper calculated the Duration Magnitude (MD) equation using data from the Iraqi Meteorological Organization and seismology (IMOS). It is an empirically determined equation and expressed as:

The epicentral distance and local geological conditions affect the duration of the coda. The data is obtained from 7 seismic stations in the network. The new proposed duration magnitude equation results from applying linear regression analysis to the data of a seismic signal duration with correlation coefficient R²=0.76; and a standard deviation value of 0.049. Station corrections indicated by Sc are also specified for the seismic stations that range from -0.024 to + 0.02.