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This study compared the effects of citric acid (CA) derived from microbial fermentation using Aspergillus
niger (400 mg kg-1) and natural extraction from Citrus limon (400 mg kg1) in albino mice. Parameters assessed included
cytogenetic damage via the micronucleus (MN) assay, immunological response through immunoglobulin (IgA, IgG,
IgM) titers, and hepatoprotective activity based on liver enzyme levels (AST, ALT, ALP) and histopathological inves
tigation. Mice were divided into six groups (n=10/group) as follows: (1) negative control, (2) carbon tetrachloride (CCl₄
0.02%), (3) lemon CA, (4) A. niger, (5) CCl₄ + lemon CA, and (6) CCl₄ + A. niger CA. The CCl4 group exhibited a
significantly higher MN frequency (0.06 ± 0.008) compa
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