The aim of the research is the detection of heavy metals using (Inductively coupled Plasma ICP) for samples in Tigris river at intakes of water treatment plants Baghdad (Sharq dejla, Al-Wathba, Al-Wahda, and Al-Dora) and samples at Tigris banks near (Al-Adhamya, Al-Shuhda bridge and al-Jadrya).
All the recorded results were fitted with Iraqi standers No. 25 in 1967 for all samples with heavy metals (arsenic Ar, Cadmium Cd, Chromium Cr, Zinc Zn, Lead Pb, Copper Cu, Nickel Ni, Manganese Mn, Ferrous Fe) where all concentration were lower than standard values except Cadmium (0.01- 0.014) in plants intakes and (0.027- 0.048) in river samples while the standard value is (0.005).
Other tests such as chemical oxygen demand and oil & grease were out of limit due to spoilled oil and illegal discharging to the river by power station and refinery units.
Variations Heavy Metals Concentrations in Tigris River in Baghdad City.: Variations Heavy Metals Concentrations in Tigris River in Baghdad City.
Heavy metals
ICP
Treatment plant
Raw water
Tigris river.
Publication Date
Mon Apr 06 2020
Journal Name
August
INFLUENCE OF SOME FACTOR ON SOMATIC EMBRYOS INDUCTION
AND GERMINATION OF DATE PALM CV BARHI BY USING CELL
SUSPENSION CULTURE TECHNIQUE
This study was done to examine different concentrations of mercuric chloride (Hgcl2) for Explants sterilization and different
concentration of NAA) mg/L with 2iP for their effect on initiation primary callus and subsequent embryogenesis in Phoenix
dactyliferae cultivar Barhi. Leaf primordial and shoot tips were excised from 2-3 years old offshoot. The result showed that
contamination percentage decreased with an increase in concentration of Hgcl2 for two explants where in the control (0.0)%
gave highest value 75% and 62.5% of contamination for leaf primordial and shoot tip respectively while the addition (0.1)%
gave a significant decrease 37.5 and 0% for explants respectively. The Explants were inoculated onto Murashiege and Skoog
1962 (MS) medium supplemented with 2iP 3mg/L and containing 0
25
50 or 100 mg/L of NAA
the addition (50) mg/L of NAA
gave highest value 68.8% in percentage of callus initiation with significant difference from addition 0% gave lowest value
(18.8)%
as well Explants of shoot tip gave significant difference was 53.1% while leaf primordial gave 28.1%
Primary callus
was transferred to fresh MS medium containing (0
5
10 and 20) mg/L of NAA with 3mg/L 2iP
Results were recorded after 12
weeks. A highest value of embryonic callus induction percentage or fresh weight and dry weight 87.5% or 1.56g and 0.47g for
the character respectively for the treat 20 mg/L while control treatment 0.0 gave the lowest value 12.5% or 0.37g and 0.14g for
same character respectively. Inoculated 1g fresh biomass into a 250 ml Erlenmeyer flask containing 50 ml MS liquid medium
supplemented with NAA 20mg/L and agitated at 150 rpm by shaker to making suspension culture the measurements of
packed cell volume ( pcv) or fresh and dry weight taken weekly for 12 weeks
maximum values were reached in the eighth week
of PCV and fresh weight but the dry weight reached to highest value in seventh week
then the curve began to decline
gradually until it reached stability in the last two weeks. The cell suspension was Diluted to different initial cell density of 100
500
10000
50000 and 100000 cells /ml with hemocytometer. The initial cell number of (10000) cells gave significant different
of plating efficiency PE (11.6)% while (100) cells gave lowest value 1.8% of PE.