This study has been performed to study the inhibitory effects of crude plant extracts of Bay (laurus nobilis) leaves against some bacterial isolates represented by Staphylococcus aureus, Staphylococcus epidermids, Proteus vulgaris, Bacillus subtilis, Escherichia coli, and Pseudomonas aeroginosa in vitro. The results showed that percentages of essential chemical of laurus nobilis leaves which represented by moisture, total oil, total ash, crude protein, crude fibers, carbohydrites and caloric values in dry weight are 5.96, 4.28, 14.2, 8.75, 24.8, 76.99%, and 284.92 kcal/100g respectively, the percentages of some major and minor mineral elements of laurus nobilis leaves powder which represented by Mg, Fe, Cu, Pb, Cd and As, are: 0.211, 0.165, 0.023, 0.011, 0.00, 0.0004 mg/g and 3.140 ppm, respectively, while Co did not appear in the laurus nobilis leaves. The aqueous extract of the plant was acidic its pH 5.79. The results of initial detection (precipitation) showed that ethyl acetate and aqueous extracts of laurus nobilis leaves contain all active compounds represented by tannins, saponins, flavonoides, glycosides, phenols, terpenoids, sterols and cumarin, while alcoholic and hexan extracts contain also the some compounds except saponins, hexane extracts no phenols. Through the study of the effect of laurus nobilis leaves extracts against growth of bacteria in vitro a significant differences at (p<0.05) was observed, It was found that the ethyl acetate and alcoholic extracts more were effective on the bacterial species than the other remaining extracts against the growth of bacteria, inhibitory activity was of all bacterial species at concentration of 3.125mg/ml and it was more effective on Staphyloccocus epidermids for ethyl acetate extract giving inhibitory zone estimated 15.5mm, while alchoholic extract was activity against bacteria E. coli at inhibition zone estimated 15mm, while hexan extracts has been showed inhibition activity of same concentration only on bacteria Staphyloccocus aureus with zone 10.5mm, as for it has been showed inhibitory activity of bacteria Proteus vulgaris at concentration 12.5mg/ml with zone 13mm, as for Staphyloccocus epidermids, Bacillus subtilis, E. coli and Pseudomonas aeroginosa were their inhibition activity at concentration 25mg/ml while Staphyloccocus aureus was highest inhibition at zone 16.5mm and 14mm of same concentration as for the hexan and aqueous extracts respectively, while the inhibition of bacteria Proteus vulgaris was zone 10mm of aqueous extract, inhibitory activity of bacteria Pseudomonas aeroginosa has been showed at concentration 50mg/ml of zone 16mm, as for the bacteria Staphyloccocus epidermids, Bacillus subtilis were the inhibitory zone at 12mm and E. coli at zone 18mm of concentration 100mg/ml. When measuring the minimum inhibitory concentration (MIC) for each bacterial species at a concentrations 6.25- 12.5mg/ml for ethyl acetate and alcoholic extracts have high inhibitory activity compared with the other concentrations, but the hexane and aqueous extracts have affected bacterial species at 12.5- 100 mg/ml, except for Staphyloccocus aureus and Bacillus subtilis, which was not affected by any of the concentrations of extracts.
The impact of different preservation procedures, including formalin fixation, ethanol preservation, and freezing, on the morphometric traits of fish preserved in museum specimens is tested in three different cichlid species: Oreochromis aureus (Steindachner, 1864), Coptodon zillii (Gervais, 1848), and Oreochromis niloticus (Linnaeus, 1758). The primary goal was to determine morphometric differences resulting from these procedures and identify correction values to reconstruct the original dimensions of the body. Our results support that shrinkage of morphological variables, such as total length (TL), standard length (SL),and head length (HL), is present in the three methods tested, depending on species, measurement, and treatment. This shrin
... Show MoreToxoplasma gondiiis an obligate intracellular protozoan parasite of the phylum Apicomplexa, and toxoplasmosis is an important disease of both humans and economically important animals. With a limited array of drugs available there is a need to identify new therapeutic compounds. Aureobasidin A (AbA) is an antifungal that targets the essential inositol phosphorylceramide (IPC, sphingolipid) synthase in pathogenic fungi. This natural cyclic depsipeptide also inhibits
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Selenium is naturally present in the human body, animals, and plants, and is one of the important elements in their growth and maintenance. Recently, the nanoform of selenium has attracted attention due to its low toxicity and a high degree of adsorption compared to its organic and inorganic forms. The current study aimed to examine the effect of Cress leaves (Lepidium sativum L.) extract in combination with selenium nanoparticles in alleviating polycystic ovary syndrome in letrozole-induced PCOS in adult female rats. Nonthermal or cold plasma was used in the synthesis of selenium nanoparticles. Subsequently, the produced nanoparticles were identified, the 30 rats were divided into 6 equal groups, the first group was healthy (negative contr
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Coffee bean contains bioactive compounds including caffeine and chlorogenic acid (CGA) that have a stimulant effect and are used for combating fatigue and drowsiness, and enhancing alertness. However, when the coffee bean was processed in the form of green coffee bean (GCB) extract, it has an unpleasant flavour and limitations instability, activity, and bioavailability. This study aimed to produce microcapsules of the GCB (Coffea canephora) ethanolic extract containing considerable amounts of the bioactive compounds for nutraceutical supplements. The GCB ethanolic extract was microencapsulated by spray drying using a whey protein concentrate (WPC) biopolimer. The particle size (PSA), morphology (SEM), and physicochemical charact
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The inhibitor property of curcuma longa L. extract in different concentrations of simulated refinery wastewater (0.05% - 2% wt) and at various temperatures (30, 35 and 40 ˚C) was investigated using weight loss method. The results showed that the presence of about 1.2 % (v/v) of curcuma extract gave about 84% inhibition indicating its effectiveness on mild steel corrosion in simulated refinery wastewater, besides the adsorption process on the mild steal surface obeyed the Langmuir adsorption isotherm.
Background: The rhizome of ginger is used in cooking and for medicinal purposes such as anti-bacterial, anti-fungal, anti-inflammatory and antioxidant. The aims of the study were to test the effect of ethanolic extract of ginger on growth, adherence and acidogenicity of mutans streptococci in comparison to chlorhexidine gluconate 0.2% and de-ionized water. Materials and methods: From saliva often volunteers (dental students 20-22 years); mutans streptococci was isolated, purified and diagnosed according to morphological characteristic and biochemical tests. Ginger was powdered and extracted, different concentrations of ginger extract were prepared. Chlorhexidine gluconate 0.2% used as a control positive; while de-ionized water was used as a
... Show MoreThe Effect of Chicken Eggshell Extract on Microhardness of Artificially Induced Dental Erosion in Permanent Teeth (In Vitro Study), Shatha A Abbas*, Alhan A Qasim
Background: Since the invention of laser in 1960, lasers have been developed and approved in many fields. Lasers can now be regarded as practical tools with unique properties that have been utilized effectively in several applications in fields of medical and biological sciences.Objectives: The aim of the current study was to preparation of vaccines (live attenuated and killed) by irradiation of the bacteria by the low level diode laser.Methods: six bacterial isolates were isolated from human samples of diabetic foot infections, which used for preparation of vaccines. The experiment was conducted on fifteen adult male rabbits; they were divided into three groups with 5 rabbits each. Blood samples were collected from the marginal ear vein
... Show Moreتعد المحاسبة بشكل عام علماً لكنها ليست من العلوم الصرفة وإنما من العلوم الإجتماعية مما يتطلب للتعامل مع المواضيع المحاسبية الأخذ بنظر الاعتبار الأشخاص المعنيين بالموضوع سواء كانوا المعدين للمخرجات المحاسبية أي المحاسبين، أو الاطراف ذوي المصالح المعنيين والمستفيدين من هذه المخرجات أي المستخدمين، ويعد المحاسب جزءاً من العملية الاجرائية نفسها وبهذا يكون دوره مزدوجاً يجمع بين كونه القائم بالبحث والقي
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This study involved the effect of the aqueous extracts of two plants, Origanum vulgare L.(1), Trigonella Foenum Graecum L. (Fenugreek) seeds(2) on the growth of cancer cell lines. Rhabdomyo sarcomas (RD) of human cell line and female intestine cells of Albino mice (L20B) in vitro System. These extracts were compared with the known anticancer drug Cis-platinum(Cis-Pt) as a positive control. The phytochemical tests were used for screening the active compounds in plants. The inhibition activity assay was used as a parameter of the cytotoxic effect of these extracts. Cancer cell lines were treated with four concentrations of Cis-platin, 31.25, 62.5, 125 and 250 ?g/ml for 72 hour exposure time. The same concentrations were used for the other ext
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