This study was aimed to isolate and identify Saccharomyces boulardii from Mangosteen fruits (Garcinia mangostana L.) by traditional and molecular identification methods To get safe and healthy foods probiotics for use, The isolates and two commercial strains were subjected to cultural, morphological and biochemical tests, The colonies of the isolates were spherical, smooth, mucoidal, dull and white to cream colour on SD agar media .The shape of cells was globose to ovoid and sometimes with budding, in a single form or clustered like a beehive. The isolates and two commercial strains were unable to metabolized galactose and lactose , Results shows that all isolates were unable to utilize potassium nitrate and not grow in the presence of (0.01%) cyclohexamide. Also the isolates and two commercial strains were identified by the Vitek 2 identification system, as S. cerevisiae with probability 90-94%, Since there are no data in this device includes S. boulardii. Because the cultural, morphological and biochemical tests didn’t provide sufficient evidence to distinguish between S. boulardii and S. cerevisiae, the probiotics strains must be identified up to genus and strain levels by internationally accepted methods, So the SbR7 isolate which shown high probiotic advantages and two commercial strains were diagnosed molecularly by using specific primers targeting sequence for the region (ITS) of the 5.8S rRNA gene Genomic DNA was isolated from SbR7 Isolate and ITS region of the 5.8S rRNA gene was amplified using PCR. PCR products was sequenced and compared with the sequence of this region in the DNA of S. boulardii available in GenBank (NCBI) using the program BLASTn. Results revealed, this isolate was almost genetically identical (99%) with S. boulardii standard strains.
Iraq alerts to the fact that it is necessary to keep pace with globalization and the need to attract foreign investment, in the hope that it will find a solution or it will be possible for it to control its economic problems, the most important of which is the problem of unemployment. incentives such as tax breaks.
So it is necessary to study those exemptions and study their role in encouraging foreign investment in Iraq.
Some authors studied modules with annihilator of every nonzero submodule is prime, primary or maximal. In this paper, we introduce and study annsemimaximal and coannsemimaximal modules, where an R-module M is called annsemimaximal (resp. coannsemimaximal) if annRN (resp. ) is semimaximal ideal of R for each nonzero submodule N of M.
Since the widespread use of the concept of human security in 1994, and as stated in the report of human development (UNDP) issued by the United Nations Development Programmer,And framed it with multiple dimensions of political, economical, social and cultural. This concept has became beyond the state and its security ,and covered all what, can threaten human life and humanitarian groups, according to the humanitarian needs in the following aspects: The economic, the food,health,environmental , individual, community and the political aspect,which totally means ,the disability of the security traditional perspective to deal with these issues.The achievement of human security,that handles the maintenance of human dignity for meeting their b
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Most media studies on the development of the agenda have focused on the relationship between the content of the media and the audience of these media, and the extent of its impact on the agenda of the media, in addition to comparing it to the extent of interest in the agenda of those means. While in the construction of the agenda, we focus on the relationship between news sources and the media and the role of these sources in the process Building the agenda.These sources are institutions, parties, pressure groups, interest groups, the head of state and other media. The impact of these sources on the process of building the agenda are shown through the process of the selection of resources by the channel and communicators, and the technic
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Background: Alcohol remains the single most significant Cause of liver disease throughout the Western World, responsible for between 40 and 80% of cases of cirrhosis in different countries. Many of the factors underlying the development of alcoholic liver injwy remain unknown, and significant questions remain about the value of even very basic therapeutic strategies.
Patients and Methods: A total number of 113 patients with ALD attending the Gastroenterology and Hepatology teaching hospital between December 2001 and December 2003 were studied for the gastrointestinal, esophagogastroduodenoscopic manifestation of alcoholic liver disease.
Results.'The most common presenting symptom was jaundice (62.8%), anorexia, weight loss (39.8-54
Multiple Sclerosis (MS) is a progressive neurological disease characterized by periods of quiescence and exacerbation, epidemiological data suggest the notion that MS is an acquired autoimmune disease caused by environmental factors, probably infectious, in genetically susceptible individuals.The submitted research was attempted to study the possible viral (Paramyxoviruses) role in MS, the sera of 57 MS patients were assayed for anti-measles and anti-mumps IgG antibodies using ELISA technique, the results were compared in order to establish the presence or absence of a significant difference regarding both number of positive cases and antibodies titer between the two groups, the results revealed that there is no in number of measles posit
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The emergence of the Internet and its transformation in the early nineties of the last century led to a means of mass communication that brought about significant structural changes in the media map.
Over the course of historical development, the public did not engage the media in its behavior in the implementation and dissemination of an idea, generalization or theory put forward by media experts or practitioners through these means to do it. Blogs represent the most important service provided by the Internet in recent years which has gained special importance in the media field. Where the word “Blogs” has several names: (alternative media), (blogging press), (popular press) and other names.
The medi
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