This study was aimed to isolate and identify Saccharomyces boulardii from Mangosteen fruits (Garcinia mangostana L.) by traditional and molecular identification methods To get safe and healthy foods probiotics for use, The isolates and two commercial strains were subjected to cultural, morphological and biochemical tests, The colonies of the isolates were spherical, smooth, mucoidal, dull and white to cream colour on SD agar media .The shape of cells was globose to ovoid and sometimes with budding, in a single form or clustered like a beehive. The isolates and two commercial strains were unable to metabolized galactose and lactose , Results shows that all isolates were unable to utilize potassium nitrate and not grow in the presence of (0.01%) cyclohexamide. Also the isolates and two commercial strains were identified by the Vitek 2 identification system, as S. cerevisiae with probability 90-94%, Since there are no data in this device includes S. boulardii. Because the cultural, morphological and biochemical tests didn’t provide sufficient evidence to distinguish between S. boulardii and S. cerevisiae, the probiotics strains must be identified up to genus and strain levels by internationally accepted methods, So the SbR7 isolate which shown high probiotic advantages and two commercial strains were diagnosed molecularly by using specific primers targeting sequence for the region (ITS) of the 5.8S rRNA gene Genomic DNA was isolated from SbR7 Isolate and ITS region of the 5.8S rRNA gene was amplified using PCR. PCR products was sequenced and compared with the sequence of this region in the DNA of S. boulardii available in GenBank (NCBI) using the program BLASTn. Results revealed, this isolate was almost genetically identical (99%) with S. boulardii standard strains.
the Reception and the Creative Reaction
The present study aims at investigating classroom verbal and nonverbal communication at the departments of English language . An observation checklist has been constructed , which is distributed into several domains that include a number of items to investigate classroom communication . Face validity and reliability coefficient have been computed. The checklist has been applied on 86 instructors at the Colleges of Education and Arts, Departments of English Language at the Universities of ThiQar, Basrah ,and Maysan . One sample t- test and Two independent sample t-test formulas have been used. Final results reveal that college instructors use verbal communication inside their classrooms and non- verbal communication has not been employed by
... Show MoreBackground: Lead (pb )is suspected to be one of the endocrine disruptors. People are exposed to high levelsof it in the environment through several ways .In the last years there was an evidence that it affects semenquality. The aim of the study is to assess the level of lead in the blood of male patients and correlate it with theseminal fluid parameters and hormonal levels.Methods: Fifty three infertile male patients, attending the infertility clinic in Baghdad Teaching Hospital fromFebruary 2016 till June 2016, participated in this study. Detailed history was taken and careful examinationwas done. Semen was collected from the patients and analyzed. Blood was withdrawn for hormonal and leadanalysis.Results: There was an increase in blood le
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Let R be a commutative ring with identity 1 ¹ 0, and let M be a unitary left module over R. A submodule N of an R-module M is called essential, if whenever N ⋂ L = (0), then L = (0) for every submodule L of M. In this case, we write N ≤e M. An R-module M is called extending, if every submodule of M is an essential in a direct summand of M. A submodule N of an R-module M is called semi-essential (denoted by N ≤sem M), if N ∩ P ≠ (0) for each nonzero prime submodule P of M. The main purpose of this work is to determine and study two new concepts (up to our knowledge) which are St-closed submodules and semi-extending modules. St-closed submodules is contained properly in the class of closed submodules, where a submodule N of
... Show MoreThe person who wants to learn. The history of Bab’is will notice a very
starnge phenomenon that they (Babis and Baha’is) resort to visions to prove their
delusion.
The believers usually reach to each other by their visions. This phenomenon
are shared among them from Al- Ahsa’ai time till the last phase of their propaganda
which is the Baha’is propaganda, and this is due to their ignorance and under
development in doctrine of islam which was widespread in Iran and the Islamic world.
The whole secrets of Babis and Baha’is movement were restricted among
particular group, most of them were Al- Rashti’s students and knew the basic
principles of their movement which deperding or (the principles) declarati
We dealt with the nature of the points under the influence of periodic function chaotic functions associated functions chaotic and sufficient conditions to be a very chaotic functions Palace
In this work the design and construction of a flash photolysis pulsed HCl laser was presented. The parameters of the pumping source and discharge current density was obtained, which sufficient to shift the flash lamp spectrum towards uv portion of spectrum. The maximum pulse laser energy parameters was measured. Total pressure and ratio of active gases to optimized the output pulse energy were measured , where at 125 mbar of total pressure and 1:7:14 Cl2:H2: He ratio, the laser energy was measured to be 200 mJ at pumping four flash lamps energy in the order of 6400J .The resonator consists of copper a near hemispherical mirror with the radius of curvature 3m coated by gold and reflectivity 98%,the output coupler sapphire mirror of
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