This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.
This study includes design and synthesis of new non-steroidal anti-inflammatory agents (NSAIDs) with expected cyclooxygenase-2 (COX-2) selective inhibition to achieve better activity and low gastric side effects. Two series of compounds have been designed and synthesized as potential NSAIDs,these are: Salicylamide derivatives (compounds 3,4,5 ) and Diflunisal derivatives (compounds 10&11). In vivo acute anti-inflammatory effect of one of the synthesized agents (compound 3) was evaluated in the rat using egg-white induced paw edema model of inflammation. Preliminary pharmacological study revealed that compound 3 exhibited less anti-inflammatory effect compared to that of aspirin after
... Show MoreSimulation Study
Abstract :
Robust statistics Known as, Resistance to mistakes resulting of the deviation of Check hypotheses of statistical properties ( Adjacent Unbiased , The Efficiency of data taken from a wide range of probability distributions follow a normal distribution or a mixture of other distributions with different standard deviations.
power spectrum function lead to, President role in the analysis of Stationary random processes, organized according to time, may be discrete random variables or continuous. Measuring its total capacity as frequency function.
Estimation methods Share with
... Show MoreThis study was conducted to determine the fungal cause and bio control of damping off and root rot of wheat plants by using pseudomonas fluorescens under greenhouse and field conditions. Results showed isolation of eight species from the soil and roots to deferent region of Baghdad government. Rhizoctonia solani (Rs) and Fusarium solani (Fs) were the predominant damping off fungus with frequency 60 and 52% respectively. Led the using of bacteria formulations such as crud suspension , pure bacteria filtration and pure living cells in culture medium inhibit all type fungi with rates ranging from 84-96% , 80- 93% and 75-88% respectively. Rs and Fs were more pathogenesis under greenhouse conditions, with incidence of 80 and 68% and disease s
... Show Moreتمتاز التعابير الحربية والعسكرية بعدة ملامح تميزها عن غيرها وتشكل بعض الصعوبات اثناء ترجمتها وذلك لاحتوائها على الرموز والمختصرات اللغوية وتدخل فيها الاصلاحات والعبارات الدارجة بين الجنود. في هذه الدراسة المختصرة حللنا وبينا أبرز تلك التعابير في اللغة الاسبانية وكيفية ترجمتها ترجمة دقيقة الى اللغة العربية. ان الهدف الاساس لهذه الدراسة هو تحليل بعض التعابير الحربية والعسكرية وذلك لأهمية معرفتها. تبين
... Show Moreالكتابة عن الأندلس بعد تسليم مدينة غرناطة يحمل في ثناياه ألم كبير، لأن الأندلسيين عاشوا تجربة إنسانية قاسية سببها الأساس غياب التسامح الديني والعدالة الاجتماعية، الأمر الذي دفعهم للثورة مطالبين بحقوقهم التي نصت عليها معاهدة تسليم المدينة. فقد عاش أولئك حياة صعبة ، فكانوا صورة للصراع بين حضارتين الإسلامية والغربية ، ويمكن تلمس ذلك الصراع من خلال دراسة ثوراتهم ضد السلطة الحاكمة التي يبدو من تتبع أح
... Show Moreملخّص البحث
نظراً لعلاقة الحشرات المباشرة بالإنسان في الكثير من الأحيان فمن الطبيعي أن يكون لها شأن مهم في الفقه الإسلامي, وتناول الفقهاء العديد من الموضوعات المتعلقة بالحشرات, ومايرتبط بها من أحكام شرعية, لذا تكفل هذا البحث بتتبع أهم ماتعلق بالحشرات من موضوعات, وأحكامها في الفقه الإسلامي بالبحث والدراسة في ثلاثة مباحث: تناول المبحث ا
... Show Moreعرف داء اللشمانيات منذ زمن بعيد وما يزال واحداً من اهم عشرة امراض معديه في العالم , بالأضافة الى كونه مشكلة من مشاكل الصحة العامة في العديد من دول افريقيا وامريكا الجنوبيه ووسط وجنوب غرب آسيا وشبه القارة الهندية كما انه يعد من الأمراض المتوطنه في العراق وقد سبب الطفيلي مشكله صحيه لا يستهان بها في جنوب العراق خصوصا في محافظه ذي قار . يسلط المقال الحالي الضوء على تاريخ المرض و اهميته في العراق.
