This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.
Basket ball game is one of the collective game that practiced widely with continuous developing .Through it has four chase .it required from it’s the player to have number of physical capable. One of these is endurance of strength in order to perform defense and attack duties. The problem is declining the level of this capable that hands inverting to defence and attack performance. The aim of research is to know the effect of using various exercises in developing endurance of strength and effect in developing two skills. dragging of defense and movement of defender player. The two researchers proposed that there are differences that have statistic function between post-test and pre-test for two experimental and control groups. The experim
... Show Moreإننا نتحدث عن عناصر السرعة مهمة وهي السرعة الانتقالية للأطراف السفلى والسرعة الحركية للذراع المسلحة والسرعة الحركية للطعن وسرعة رد الفعل وسرعة الاستجابة في أداء واحد او ما يسمى بالسرعة التفاعلية؛ لذا هدفت الدراسة الى اعداد تدريبات خاصة تتميز بتمازج أنواع السرعة التي تحتاجها رياضة المبارزة في أداء واحد ومن ثم معرفة تأثير تلك التدريبات في تطوير بعض الجمل الحركية في سلاح الشيش وقد استعمل المنهج التجريبي
... Show Moreهدف البحث إلى بناء مقياس التفكير المنتج بالجمناستك لطالبات، وأعتمد المنهج الوصفي بأسلوب العلاقات الارتباطية على عينة من طالبات المرحلة الثانية في كلية التربية البدنية وعلوم الرياضة للبنات/جامعة بغداد للعام الدراسي (2023/2024) البالغ عددهن الكلي (38) طالبة، وتم اختيار العينة بطريقة عمدياً بأسلوب الحصر الشامل بنسبة (100%) من المجتمع الأصل، واختير منهن (5) طالبات عشوائياً للعينة الاستطلاعية، وتم معالجة النتائج أل
... Show Moreوهو بحث تجريبي أجري على عينة من اللاعبين فئة الناشئين لكرة اليد وبواقع (20) لاعب إذ تم إعداد برنامج متضمن للتمرينات الخاصة بالهجوم الفردي بهدف تطوير بعض القدرات البدنية واللياقة القلبية للاعبين . وتكمن أهمية البحث في استخدام تمرينات الهجوم الفردي، فمن أجل الارتقاء بالمستوى البدني والوظيفي للاعبين من خلال رفع مستوى الأداء البدني لبعض المهارات في لعبة كرة اليد فضلاً عن رفع مستوى اللياقة القلبية وم
... Show MoreThis study is conducted to identify the microbial content of some types of infant milk formula available in the local markets of the city of Baghdad and their conformity microbial limits sited by the Iraqi standard. Seventy samples were collected from trademarks of imported infant milk formula included of five samples of infant milk formula No (1) and five samples of follow-up formula No (2). These samples were collected randomly from shops in the local markets of Baghdad city on both sides of Karkh and Rusafa included the following kinds: Dialac 1, Dialac 2 ,Celia 1, Celia 2 ,Biomil 1, Biomil 2 , Nactalia 1, Nactalia 2, Novalac 1 , Novalac 2 , Similac 1 , imilac 2 , Guigos 1, Guigos 2. Some microbial tests were done which in
... Show MoreThis paper presents the synthesis and study of some new mixed-ligand complexes containing anthranilic acid and amino acid phenylalanine (phe) with some metals . The resulting products were found to be solid crystalline complexes which have been characterized by using (FT-IR,UV-Vis) spectra , melting point, elemental analysis (C.H.N) , molar conductivity . The proposed structure of the complexes using program , chem office 3D(2000) . The general formula have been given for the prepared complexes : [M(A-H)(phe-H)] M(II): Hg(II) , Mn(II) ,Co(II) , Ni(II) , Cu(II) , Zn(II) , Cd(II) . A = Anthranilic acid = C7H7NO2 Phe = phenylalanine = C9H11NO2
Thispaperpresentsthesynthesisandstudyofsomenewmixed-liagnd complexescontainingtowaminoacids[Alanine(Ala)andphenylalanine(phe)]withsome metals .Theresultsproductswerefoundtobesolidcrystallinecomplexeswhichhave been characterized by using (FT-IR,UV-Vis) spectra , melting point, elemental analysis (C.H.N) , molar conductivity and solubiltyThe proposed structure of the complexes using program , chem office 3D(2000) .The general formula have been given for the prepared complexes :[M(A-H)(phe-H)]M(II): Hg , Mn ,Co , Ni , Cu ) , Zn , Cd(II) .Ala = Alanine acid = C3H7NO2Phe = phenylalanine = C9H11NO2
This paper presents the synthesis and study of some new mixed-liagnd complexes containing tow amino acids[Alanine(Ala) and phenylalanine (phe)] with some metals . The results products were found to be solid crystalline complexes which have been characterized by using (FT-IR,UV-Vis) spectra , melting point, elemental analysis (C.H.N) , molar conductivity and solubilty The proposed structure of the complexes using program , chem office 3D(2000) . The general formula have been given for the prepared complexes : [M(A-H)(phe-H)] M(II): Hg , Mn ,Co , Ni , Cu ) , Zn , Cd(II) . Ala = Alanine acid = C3H7NO2 Phe = phenylalanine = C9H11NO2