This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.
В статье рассматривается вопрос об использовании мультимедийных средств для оптимизации процесса формирования коммуникативной компетенции в иракской аудитории с привлечением компьютерных технологий. Статья посвящена использованию мультимедийных технологий и различных приемов формирования интереса к русскому языку. Включение в процесс обучения коммуникативно-значимого, аутентичн
... Show MoreEncouraging micro-enterprises for comprehensive economic development are crucial to achieve the ambitious vision 2030 of the Kingdom of Saudi Arabia.
Small and Medium enterprises are inputting around 15.5 per cent to GDP while 33 per cent contribution as a private sector to Saudi Arabia's gross domestic product (GDP). This study aims to identify the most important factors that affect the efficiency of small enterprises in Saudi Arabia. To accomplish this objective, the study was conducted for small projects via the comprehensive inventory method under the supervision of the Institute of Entrepreneurship. A total of 282 questionnaires were collected from entrepreneurs and the differentiation analysis
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Abstract
The current study aims to identify university students' attitudes towards reading and its relationship to some demographic variables in the universities of the Sultanate of Oman. The study sample consisted of (1434) male and female university students from various Omani public and private universities affiliated with the Ministry of Higher Education. The study covered all (11) governorates of Oman. The researcher adopted the descriptive analytical approach. The researcher employed a scale of reading attitudes to collect the needed data. The study results showed that university students' reading attitudes recorded a high degree. The results also showed there are statistically significant differences at th
... Show More:يمتاز مرض قصور الدرقية بإنخفاض مستوى هرمونات T3و T4الضرورية لعمليات إنتاج الطاقة و حيوية الجسم ؛ لذلك ترافق هذه الحالة إضطرابات في عمليات الأيض المختلفة في الجسم . أجريت الدراسة في المركز التخصصي للغدد الصم و السكري في بغداد ، و شملت 70ًاضيرممن مرضى قصور الدرقية ، تم تشخيص الحالة لديهم عن طريق قياس مستويات الهرمونات (TSH,T4,T3). كما شملت 60اوريتخأًاصخشبشكل عشوائي من غير مرضى الغدة ، و قد تم التأكد من سلامة الغدة وعدم
... Show Moreان ايجاد وسائل و طرائق تدريبية وتمارين مستحدثة بالاعتماد على مختلف العلوم وانعكاس ذلك على الافكار الجديدة ولعل واحدة من هذه التمارين اسلوب التمرينات الساكيو (S.A.Q) وتمرينات الساكيو من التمرينات الحديثة المستخدمة في المجال الرياضي كونها تحسن من كفاءة الأداء اما مشكلة البحث لاحظت الباحثتان هناك هبوط في انجاز100م حواجز, وهذا يتطلب التقصي والبحث عن أسباب هذا الهبوط والوقوف على نقاط الضعف الواضحة في مراحل الع
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إن الأبحاث العلمية كانت و لا تزال تحضا باهتمام الكثير من العلماء و المختصين في مجال التربية الرياضية بشكل عام و الجمناستك بشكل خاص لإيجاد أحدث الوسائل و الأدوات التي تساعد على سهولة توصيل المعلومات إلى المتعلمين و بدرجة عالية من الكفاءة, إذ يمكن للمتعلم الاستعانة بوسائل تعليمية عديدة منها القراءات المختلفة و المواد المبرمجة أو الأفلام و الوسائل السمعية و البصرية و ما إلى ذلك, فالمعلم الذي يستخدم هذه الوسائل
... Show MoreThe aim of the research is to investigate potential effects of the finance industry and block-chain to general business of financing in particular, as well as its shortcomings and difficulties. To answer the research questions, the researcher used the objective narrative-analytical descriptive approach and included a qualitative analysis of Blockchain technology. The process of Blockchain technology based on their industries, the authors were selected based on their reputation in the Blockchain field. The research found that Blockchain can improve the efficiency of the banking industry's various sections. It has the ability to upgrade and transfer wages across borders, financial reporting and compliance, as well as trade finance
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The current research is concerned with the prices of Goods and materials in the Iraqi slang a descriptive, lexicographic , and semantic study expressing the meanings of these names and their positions , as well as expressing the imaginations of Human mind , the popular mind in describing these goods with evaluating them besides the semantic of each word accordingly
The current research is divided into two parts , the first part is consisted of Vocalizations" words" That are arisen through cognitive naming that concentrate on the mental imaginations for the most important and sensitive such as colors , taste , shapes and forms impacts of Goods and materials according to users' ' taste for those words , on other hand, the second part of
This study was carried out in order to determine the toxic, mutagenic and antimutagenic effects for Mallow (Malva parviflora) in comparison to its mutagenic effect of Ultraviolet (UV) because it is consider physical mutagen by using parameters for the extract pri , with , post UV exposure by using bacterial system (G-system). The used system consisted of three isolates G3 Bacillus spp., G12 Arthrobacter spp. and G27 Brevibacterium spp.. The study depended on recording survival fraction (Sx) for studying the effects and induction of Streptomycin and Refampicin resistance mutants as a genetic markers.Water Extract was prepared from fresh and dry mallow leaves, stems, flowers and roots, in optimum concentration equal to (125µg/ml) which is
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