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STUDY THE INHIBITION ACTIVITY OF PURIFIED BACTERIOCIN FROM LOCAL ISOLATION Lactococcuslactis ssp. lactisagainst SOME PATHOGENIC BACTERIAL SPECIES ISOLATED FROM CLINICAL SAMPLES: دراسة الفعالية التثبيطية للبكتريوسين المنقى من العزلة المحلية Lactococcuslactis ssp. Lactis تجاه بعض البكتريا الممرضة المعزولة من عينات سريرية
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This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.

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Publication Date
Wed Jan 01 2014
Journal Name
Journal Of The College Of Languages (jcl)
Learning English through Scaffolded Assistance in Iraqi EFL Classroom
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Learning a foreign language is a highly interactive process, and a belief that communicative activities foster a great amount of linguistic production provides language practice and opportunities for negotiation of meaning during communicative exchanges. Thus, this study examines what benefits learner-centered classroom setting offers compared with that of teacher–centered classroom, and how less proficient learners accomplish their tasks and activities with scaffolded help during interaction with the help of proficient classmates and under the guidance of a skilful person, i.e., the teacher. The subjects participating in this study are 30 Iraqi 4th year college students in the Department of English, College of Arts , Univer

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Publication Date
Wed Jun 01 2005
Journal Name
Journal Of The College Of Languages (jcl)
המאבק הפנימי של מרדכי זאב פיארברג מתוך גיבורו של הסיפור "לאן?" מורה עוזר זינה אייד קסים
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המחקר הזה עוסק באחד מהסופרים העבריים שנחשבו בעלי־השפעה בתהליך הספרות
העברית החדשה על אף גילו הקצר ועל אף שהוא השאיר עזבון ספרותי מועט , אך עם זה דמותו עולה
לענינו גם מתוך כתביו המעטים כאילו הם טבועים בחותם, הסופר הזה הוא מרדכי זאב פיארברג.
במחקר זה טיפלנו בחייו של המספר הזה בקיצור וגם ביצירותיו באופן כללי, ובסימנים המאפינים
את גיבוריו, ובמיוחד גיבורו של הסיפור "לאן?" שהוא אכן גיבור פיארברגי מובהק והוא נת

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Publication Date
Thu Mar 30 2023
Journal Name
مجلة نسق
واقع التمكين الاداري في كلية التربية للعلوم الصرفة - ابن الهيثم من وجهة نظر موظفيها
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Publication Date
Wed Mar 30 2022
Journal Name
حوليات أداب عين شمس
الاعلال في الجزء الثلاثين من القرآن الكريم في كتاب الجدول في إعراب القرآن الكريم
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Publication Date
Tue Jan 01 2019
Journal Name
مجلة مداد الاداب
معوقات تطبيق تكنولوجيا المعلومات والاتصالات في كليات جامعة بغداد من وجهة نظر العمداء والمعاونيين
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هدف البحث التعرف على المعوقات التي تحول دون تطبيق تكنولوجيا المعلومات والاتصالات في كليات جامعة بغداد بأعتبار أن ذلك يمثل مشكلة لابد من الوقوف عندها , ولأهمية مثل هذه المواضيع وضرورة البحث عنها , إذ تحدد مجتمع البحث بالعمداء والمعاونيين في هذه كليات جامعة بغداد للعام الدراسي 2016-2017حيث قام الباحث ببناء اداة خاصة للبحث لغرض الاجابة على هدف البحث الرئيسي ومن خلال أجابات أفراد عينة البحث التي مثلت ما نسبته66% الى ا

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Publication Date
Sun Jan 04 2015
Journal Name
Journal Of Educational And Psychological Researches
مهمات مديري المدارس في ضوء نظام المدارس الثانوية في العراق من وجهة نظر المدرسين
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The current research aims know:

1.-level application managers secondary schools and junior high for the tasks set by the high school system in Iraq, from the standpoint of teachers.

  1. statistically significant differences in the extent of the application of secondary school principals and junior high for the tasks set by the high school system from the viewpoint of teachers depending on the variable (sex, specialty, number of years of service).

The researcher used the descriptive analytical method being considered best suited to such studies, for the purpose of achieving the goals of research, the researcher using the questionnai

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Publication Date
Sun Feb 03 2019
Journal Name
Journal Of The College Of Education For Women
تقويم جودة واداء خدمة الانترنت من وجهة نظر مالكي مقاهي الانترنت في محافظة بغداد
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Publication Date
Wed Apr 03 2013
Journal Name
كلية اللغات-جامعة بغداد
مشاكل ترجمة المصطلحات السياسية وتنوعها من العبرية الى العربية (مشروع الدبلوم العالي المعادل للماجستير)
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Publication Date
Wed Jul 01 2015
Journal Name
Journal Of Educational And Psychological Researches
مستوى التنمية المهنية لتدريسيي جامعة بغداد في ضوء مؤشرات الجودة الشاملة من وجهة نظرهم
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The present study aimed to know The teaching members’ level of the professional development at the University of Baghdad based on the indicators of the overall quality from their perspective;

The researchers used the descriptive and analytical approach the in order to achieve the purposes of research, the researchers designed questionnaire researched and consisted of 40 paragraph has the questionnaire submitted to the arbitrators and experts were verify the authenticity of the questionnaire and stability have been applied questionnaire on a range of teaching and Tdriciat Baghdad University, which reached the study sample (400) teaching and teaching of six colleges in the University of Baghdad equivalent

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Publication Date
Sun Apr 28 2019
Journal Name
Arab Science Heritage Journal
الظواهر الظواهر الاجتماعية في بغداد من خلال كتاب الظرف والظرفاء لابي الطيب الوشاء(ت320هـ)
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 the study of social phenomena in this direction is, the sign of deviousness to the segments of the circumstance with their conditions and circumstances, then pointed to the uniforms that they were adorned by the tortillas as well as shirt Safaf from good linen soft and touched the country and the good and others.

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