This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.
مشكلة البحث:
يبقى الفن ولا زال في جميع حالات التعاقب البشري ومراحل التطور الانساني الاكثر انطلاقة وتميزاً في التعبير عن واقع الانسان وعن مشاعره وانفعالاته وافكاره ذات الصلة بتأثيرات البيئة المحيطة به.
والفن ولاسيما (الرسم) يمثل وسيلة من وسائل التعبير الفني بل يكاد يكون الرسم وسيلة الانسان الاولى التي عبر فيها بخطوط مرئية عن مجالات حياته وعلاقته بالبيئة التي عاش فيها، ويقينا
... Show MoreExpired drug Metoclopramide was investigated as an antibacterial corrosion inhibitor for carbon steel in 0.5M H3PO4 solution using the electrochemical method at 30oC and 60oC. The results showed that this drug is an efficient inhibitor for carbon steel and the efficiency reached to 82.244 % for 175 ppm at 30oC and 76.146% for 225 ppm at 60oC. The adsorption of drug on carbon steel surface follows Langmuir adsorption isotherm with small values of adsorption-desorption constant. The polarization plots revealed that Metoclopramide acts as mixed-type inhibitor. Some parameters of inhibition process were calculated and discussed. The surface morphology of the carbon steel speci
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هدفت الدراسة التعرف على الضغوط النفسية والخوف من المستقبل لدى أمهات أطفال طيف التوحد، بلغت عينة الدراسة )60( ام لديهن طفل توحدي، تم استخدام المنهج الوصفي التحليلي وبعد تطبيق مقياسي الدراسة ومعالجة البيانات احصائيا توصلت الدراسة ان األمهات لديهن درجة عالية من الضغوط النفسية ودرجة عالية من الخوف حول مستقبل اطفالهن، وفي ضوء نتائج الدراسة خرجت الباحثة ببعض التوصيات والمقترحات.
The research endeavored to single out the problems, difficulties and obstacles faced by the members of the staff of Baghdad University. The importance of research, then, depends upon the importance of the university lecturers and their vital role in developing the society. The goals of the research are summarized in knowing the main problems facing those university lectures and relative weight of these problems in addition to identifying the differences in their opinions to these problems according to the specializations variables (scientific, humanistic), the gender (males, females) and the Scientific grade (professor, assistant professor, lecturer, assistant lecturer.
The research is limited
... Show MoreThis study aimed to discover the reality of the teacher's maintainced of order and classroom management from the perspective of teachers themselves.
A social survey with an introduce sample that consist (180) male and female teachers from the provinces of Ajloun and Jerash in 2008-2009. To achieve the objective of the study was developed for this purpose and the results have shown the existence of statistically significant differences in marinating order and the management of class and in favor of females, the results also showed Statistically significant differences regarding the academic qualification and for the benefit of holders of master degrees and more compared to those with bachelor's degree.
... Show Moreهدفت الدراسة الى تحديد الكفايات التدريسية اللازمة للطالبات- المدرسات من وجهة نظرهن وذلك للتعرف على مدى حاجتهن لهذه الكفايات التدريسية التي ينبغي امتلاكها لتحقيق الاهداف المنشودة.أشتملت عينة البحث على(100) طالبة من جامعة بغداد/كلية التربية للبنات/قسم التاريخ.تم اعداد اداة البحث وهي أستبانة مكونة من (43) فقرة خماسية البدائل هي (موافقة بشدة، موافقة،مترددة،غير موافقة،غير موافقة بشدة) موزعة على
... Show MoreOffline handwritten signature is a type of behavioral biometric-based on an image. Its problem is the accuracy of the verification because once an individual signs, he/she seldom signs the same signature. This is referred to as intra-user variability. This research aims to improve the recognition accuracy of the offline signature. The proposed method is presented by using both signature length normalization and histogram orientation gradient (HOG) for the reason of accuracy improving. In terms of verification, a deep-learning technique using a convolution neural network (CNN) is exploited for building the reference model for a future prediction. Experiments are conducted by utilizing 4,000 genuine as well as 2,000 skilled forged signatu
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Acquires Find importance of the overall quality and e-marketing management have become important factors in evaluating the performance of banks, which are related to the life of the community intimately, so it is important that the banks applying comprehensive quality and e-marketing management requirements in order to maintain their performance and determine their level, as well as the manifest importance of research in part, practical linking the requirements of total quality management and banking performance on the one hand and between the e-marketing and performance banking on the other hand, through the provision of scientific bases that can be based on the banks in question, as it kicks off the research problem in that mos
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Increased the need to promote the profession of auditing, and improving the performance of the audit process, which comes through the effective implementation of its tasks, where the auditor collects the necessary data about the nature of client activity, and any other information it deems necessary to carry out the planning and development of the strategy for how to determine the procedures for implementing the audit process and the scope and timing for evidence clues in order to form an opinion technician neutral about the fairness of the financial statements, as required by the standards of the field work of the planning and supervision of the internal control and evidence to prove, but the failure of the auditor whether in th
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The subject of marketing culture and mental image is one of the important topics in the field of management. There is no study that combines these two variables. The research is important because of the increasing importance of the subject. The future direction of the company in question will support the company's economic and marketing responsibilities. And reflect the company's mental image, as a culture that contributes to changing the reality of the organization investigated by polling the views of a sample of managers in the General Company for Vegetable Oil Industry, which (30) out of the (65) individual, and There are two hypotheses of research: There is a significant
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