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STUDY THE INHIBITION ACTIVITY OF PURIFIED BACTERIOCIN FROM LOCAL ISOLATION Lactococcuslactis ssp. lactisagainst SOME PATHOGENIC BACTERIAL SPECIES ISOLATED FROM CLINICAL SAMPLES: دراسة الفعالية التثبيطية للبكتريوسين المنقى من العزلة المحلية Lactococcuslactis ssp. Lactis تجاه بعض البكتريا الممرضة المعزولة من عينات سريرية
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This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.

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Publication Date
Mon Dec 05 2022
Journal Name
Baghdad Science Journal
Effects of Castor Oil Nanoemulsion Extracted by Hexane on the Fourth Larval stage of Culex quinquefsciatus from Al Hawizeh Marsh/Iraq, and Non- Targeted Organism
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           The current study aims to show the importance of plant products as mosquitocides against Culex quinquefasciatus. Castor oil Nanoemulsions were subedit in various ratios including castor oil, ethanol, tween 80, and deionized water by using ultrasonication. Thermodynamic, centrifugation, PH, assay which improved that the formula  of 10 ml  of castor  oil, ethanol  5ml, tween 80 (14 ml) and deionized water 71ml was more stable than other formulas. The stable formula of castor oil nanoemulsion was characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS). Nanoemulsion droplets were spherical in shape and were found to have a Z-average diameter of 87.4nm. A concentration of ca

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Publication Date
Fri Oct 30 2020
Journal Name
Journal Of Economics And Administrative Sciences
Development of human resources and their role in achieving artificial intelligence A survey of the views of a sample of workers in the cement plant
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The research topic was chosen as a result of the importance of human resource in business organizations in general and the industrial process in particular. Without the human resource, business organizations cannot continue and achieve success and excellence, and the research problem has been diagnosed in the lack of sales of General Cement Company’s northern products, despite their distinctiveness, standing, and reputation in The market and its products with standard specifications, and through this problem, the following questions were raised:                                                    &nbs

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Publication Date
Sun Jun 16 2019
Journal Name
Al–bahith Al–a'alami
News frames for the phenomenon of terrorism in international satellite channels directed in Arabic An analytical study of the main news bulletins in the Russian channel today 1/2/31/5/2018
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International satellite channels in Arabic are targeted to the region with their news bulletins and their innovative programs, attracting the interest of the Arab viewers in their news articles and programs with new ideas and methods as well as high technology in the production and synthesis of videos. Therefore, they work on framing the above, And the media policy that governs, so focused the problem of the study to try to answer the question of the President is how to frame the international satellite channels in Arabic to the phenomenon of terrorism? What are the aspects that are working to highlight and aspects that try to hide? The study adopted the survey methodology for the main news bulletins in the Russian channel today for the

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Publication Date
Fri Mar 25 2011
Journal Name
مجلة جامعة النهرين
فعالية انزيم الالنين امينو ببتيديز ومتناظراته المنقاة جزئيا من ادرار المرضى المصابين بسرطان الجهاز البولي
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Publication Date
Thu Aug 01 2019
Journal Name
Journal Of Economics And Administrative Sciences
تحسين " مقدرات المتغيرات المساعدة بطريقة جاكنايف " باستعمال صنف من أصناف خوارزمية المناعة مع تطبيق عملي
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تستند أغلب الطرائق الحصينة على فكرة التنازل عن جانب معين مقابل تقوية جانب آخر من خلال عدة أساليب أما آليات الذكاء الصناعي تحاول عمل موازنة بين الضعف والقوة للوصول إلى أفضل الحلول بأسلوب بحث عشوائي . في هذا البحث تم تقديم فكرة جديدة لتحسين مقدرات معلمات نماذج المعادلات الآنية الخطية الناتجة من طريقة المتغيرات المساعدة حسب طريقة جاكنايف Jackknife Instrumental Variable Estimation(JIVE) وذل

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Publication Date
Fri Jun 12 2026
Journal Name
Kufa Journal Physical Education Sciences
العدالة التنظيمية للهيئات الإدارية واسهامها بالتميز المنظمي للأندية الرياضية العراقية من وجهة نظر مدربي التايكواندو
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Publication Date
Wed Dec 05 2018
Journal Name
Al-adab Journal
الجنس والتأنيب في الوسط الاكاديمي: ادلة من الوسط الاكاديمي العراقي لمتعلمي اللغة الانكليزية لغة اجنبية
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تتقصى هذه الدراسة الاساليب التداولية للتأنيب لدى لطلبة العراقيين الجامعيين متعلمي اللغة الانكليزية لغة اجنبية من كلا الجنسين (ذكورا واناث) وتحديد الاساليب التداولية التي يفضلها كل منهما. تم اختبار اربعين طالبا مقسمين بالتساوي من كلا الجنسين تحريريا بأستخدام فرض اكمال النص. أظهرت النتائج بأن الطلبة العراقيين من كلا الجنسين يفضلون اسلوب التأزرعلى التمايز وتفضيل استخدام الالفة والصداقة من دون الاخلال با

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Publication Date
Thu Aug 01 2019
Journal Name
Journal Of Economics And Administrative Sciences
مقارنة مقدرات بيز لدالة المعولية لتوزيع باريتو من النوع الاول باستعمال دوال معلوماتية مضاعفة مختلفة
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The comparison of double informative priors which are assumed for the reliability function of Pareto type I distribution. To estimate the reliability function of Pareto type I distribution by using Bayes estimation, will be  used two different kind of information in the Bayes estimation; two different priors have been selected for the parameter of Pareto  type I distribution . Assuming distribution of three double prior’s chi- gamma squared distribution, gamma - erlang distribution, and erlang- exponential distribution as double priors. The results of the derivaties of these estimators under the squared error loss function with two different double priors. Using the simulation technique, to compare the performance for

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Publication Date
Fri Jun 30 2023
Journal Name
Modern Sport
بناء و تقنين مقياس القيادة الخادمة لمديري المدارس الثانوية من وجهة نظر مدرسي التربية الرياضية
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تواجه المؤسسات التربوية تحديات وضغوطات بسبب التقدم السريع في المجالات العلمية و التقنية و التي هي سمة عصرنا مما جعلت مدير المؤسسة يبحث عن اساليب حديثة وطرق جديدة للادارة لقيادة و ادارة المؤسسة  ومنها القيادة الخادمه، وتنبثق اهمية البحث من  كون مدير المدرسه هو الاساس للعملية التربوية والتعليميه التي لها تاثير على مخرجات العملية التعليمية   اما مشكلة البحث فقد تجلت بعد ان وجهت الباحثتان استبانه الى عدد

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Publication Date
Mon Jan 01 2024
Journal Name
مجلة رماح للبحوث والدراسات
موقف مصر السياسي من أزمة سد النهضة الإثيوبي خلال حكم الرئيس حسني مبارك 1981-2011
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