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STUDY THE INHIBITION ACTIVITY OF PURIFIED BACTERIOCIN FROM LOCAL ISOLATION Lactococcuslactis ssp. lactisagainst SOME PATHOGENIC BACTERIAL SPECIES ISOLATED FROM CLINICAL SAMPLES: دراسة الفعالية التثبيطية للبكتريوسين المنقى من العزلة المحلية Lactococcuslactis ssp. Lactis تجاه بعض البكتريا الممرضة المعزولة من عينات سريرية
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This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.

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Publication Date
Sat Dec 01 2018
Journal Name
Journal Of Economics And Administrative Sciences
Organizational Tactics An Approach to Strategic Agility A field study in a sample of mobile operators in Iraq
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The current research dealt with the issue of organizational skillfulness as an entry point to reach strategic agility. The study has been tested in Iraq's mobile operators - Asia Cell, Zain Iraq and Cork Telecom. The study was applied to a sample of (93) managers distributed at various levels of management (board members, general managers, commissioners, department managers, people managers, unit managers, office managers). The survey used the questionnaire as a key tool for collecting data and information as well as personal interviews. It has sought to test a number of hypotheses related to correlation and influence relationships between the variables of the study, in order to answer the questions related to the problem of stud

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Publication Date
Wed Dec 29 2021
Journal Name
Journal Of The College Of Education For Women
The Degree of Secondary School Managers' Practice of Servant Leadership in Gaza from the Teachers’ Perspective : رندة عيد شرير, و سحر محمد حرب
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Abstract

The study aims to know the degree of secondary school managers' practice of servant leadership in Gaza from their teachers’ perspective. It further aims to examine whether there are any statistically significant differences at (α 0.05≥) between the means of the study sample’s members attributed to variables such as (gender, educational qualification, and years of experience). To achieve the objectives of the study, the study adopted the descriptive survey method, using a questionnaire consisting of (30) items distributed to four domains: (workers service, ethical dealing, motivation & influence, and building an organized, integrated society). Then, the questionnaire was distributed to  t

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Publication Date
Tue Mar 01 2005
Journal Name
Albahith Alalami
الجوانب الدعائية في السينما العالمية اثناء النصف الاول من القرن العشرين
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شهدت السينما اثناء عمرها الممتد عبر نحو قرن من الزمن احداثا وتطورات سياسية مهمة، فضلا عن التطورات التقنية والفنية وكانت السينما حاضرة وشاهدة على احداث سياسية بارزة مر بها العالم قبل واثناء وبعد الحربين العالميتين الاولى والثانية وفترة الحرب الباردة وما بعده. وقد حظيت السينما اثناء النصف الاول من القرن العشرين باهتمام كبير من قبل الدول الكبرى والسياسيين فيها فضلا عن الاقبال التجاري للشرك

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Publication Date
Sun Dec 06 2015
Journal Name
مجلة ابن الهيثم للعلوم الصرفة والتطبيقية
فصل وتنقية جزئية لأنزيم الارجنيز من امصال النساء المصابات بتليف الرحم
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Publication Date
Fri Dec 30 2022
Journal Name
Modern Sport
علاقة الثبات والتوازن بدقة مهارة التصويب من القفز عاليا بكرة اليد
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تلخصت مقدمة البحث بأن التحليل  الحركي الميكانيكي وسيلة مهمة بواستطتها يتم تشخيص وتقيم الظاهرة بشكل موضوعي وفق أسس علمية ثابتة ،بأستخدام التقنية الحديثة، وكرة اليد من بين الألعاب التي ظهرت في الآونة الأخيرة بمستوى أداء يجذب إعجاب المشاهد لهذه اللعبة، كماأن الدقة في التصويب تعد مفتاح لتحقيق الهدف من خلال وصول الكرة إلى المكان الصحيح ودخولها إلى المرمى قبلَ أن تكون فرصة للصد . و مشكلة البحث بان اللاعب يهدر

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Publication Date
Tue Jan 01 2002
Journal Name
Al-adab Journal
تقويم برنامج التربية العملية في جامعة بغداد من وجهة نظر الطلبة
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Publication Date
Sat Mar 30 2019
Journal Name
College Of Islamic Sciences
لمحات حقوق الإنسان الفكرية من مقاصد فقه العبادات: (أركان الإسلام مثالاً(
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ملخـــص البحــــث

 

ان الحكمة من تشريع العبادات هي ربط المخلوق بالخالق بصورة دائمة ومستمرة لأجل سعادة هذا المخلوق في الدنيا والاخرة ، فكل عبادة فيها من الغايات والمقاصد التي تحفظ للإنسان فكره وعقله واخلاقه وكرامته وكل ما يملك من حاجات مادية ومعنوية وروحية ، فالعبادات وخاصة اركان الاسلام هي تشريف وتكريم للعباد قبل ان تكون تكليفا ، يسمو الانسان من خلالها فكريا وعقليا وقلبيا

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Publication Date
Sun Jan 01 2023
Journal Name
حوليات أداب عين شمس
تطبيق نظرية العلاقات البلاغية في تحليل اثنتين من السوناتات الرومانسية الإنجليزية
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Publication Date
Sat Jun 30 2001
Journal Name
Iraqi Journal Of Chemical And Petroleum Engineering
تنقية الهكسان الصناعي من المركبات العطرية لغرض استخدامه في الصناعات الغذائية
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Publication Date
Tue May 16 2023
Journal Name
Journal Of Engineering
تحولات الدار السكني العراقي في العقد الاول من القرن الواحد والعشرين
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This research describes Iraqi housing in the first decade of the twenty-first century, taking ALAdhamiya
as a model, depending on descriptive method of inductive approach to the study of this decade.
By doing the field study for three neighborhood in Adhamiya district. Which takes in consideration the
comparison between housing in the first period of the first decade of the twenty-first century with the
second period of this decade: the latest decades of 21th century. And for many aspects involving areas,
sizes, forming, direction of opening of housing and the function, to reach the most important transformation
of Iraqi housing in the first decade of this century in order to give a picture of the residential fact for t

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