This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.
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Background: Decontamination of gutta percha cones was important factor for success of root canal treatment. The aim of the present in vitro study was to identify and to compare the antimicrobial effect of following disinfection solutions: 0.2% chlorhexidine gluconate, Iodine, tetracycline hydrochloride solution, EDTA & formocresol mixed with zinc oxide eugenol, on E faecalis, E coli and Candida albicans using sensitivity test Materials and Methods: Three types of microorganisms were isolated from infected root canals (E faecalis, E coli and Candida albicans) and cultured on Mueller Hinton agar petri-dishes. Disinfection of gutta percha cones done by immersion in six disinfection solutions (six groups), the groups are: distill water (used a
... Show Moreتلخصت مقدمة البحث بأن التحليل الحركي الميكانيكي وسيلة مهمة بواستطتها يتم تشخيص وتقيم الظاهرة بشكل موضوعي وفق أسس علمية ثابتة ،بأستخدام التقنية الحديثة، وكرة اليد من بين الألعاب التي ظهرت في الآونة الأخيرة بمستوى أداء يجذب إعجاب المشاهد لهذه اللعبة، كماأن الدقة في التصويب تعد مفتاح لتحقيق الهدف من خلال وصول الكرة إلى المكان الصحيح ودخولها إلى المرمى قبلَ أن تكون فرصة للصد . و مشكلة البحث بان اللاعب يهدر
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قمنا بتحليل مراحل تطور الرواية الروسية في القرن التاسع عشر. وبحثنا في طرائق تصوير الواقع الروسي وما بعد الواقعية. ونحاول الإجابة عن الأسئلة الآتية: لماذا سُمّيَ القرن التاسع عشر بالقرن الذهبي؟ ولماذا في النصف الثاني من القرن التاسع عشر أصبحت الرواية الروسية واجهة للحركة الأدبية في روسيا؟ وتجدر الإشارة إلى أن الرواية الروسية خلال هذه الحقبة وصلت إلى أعماق النفس البشرية بطريقة غير مسبوقة، عن طريق عر
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ملخـــص البحــــث
ان الحكمة من تشريع العبادات هي ربط المخلوق بالخالق بصورة دائمة ومستمرة لأجل سعادة هذا المخلوق في الدنيا والاخرة ، فكل عبادة فيها من الغايات والمقاصد التي تحفظ للإنسان فكره وعقله واخلاقه وكرامته وكل ما يملك من حاجات مادية ومعنوية وروحية ، فالعبادات وخاصة اركان الاسلام هي تشريف وتكريم للعباد قبل ان تكون تكليفا ، يسمو الانسان من خلالها فكريا وعقليا وقلبيا
... Show MoreThis research describes Iraqi housing in the first decade of the twenty-first century, taking ALAdhamiya
as a model, depending on descriptive method of inductive approach to the study of this decade.
By doing the field study for three neighborhood in Adhamiya district. Which takes in consideration the
comparison between housing in the first period of the first decade of the twenty-first century with the
second period of this decade: the latest decades of 21th century. And for many aspects involving areas,
sizes, forming, direction of opening of housing and the function, to reach the most important transformation
of Iraqi housing in the first decade of this century in order to give a picture of the residential fact for t
(1)