Lactobacillus Plantarum and Lactobacillus rhamnosus GG were encapsulated using 3% of alginate via extrusion technique. And the probiotics capsules produced were further coated used 1% chitosan to increase the survival of probiotics, and evaluation of The heat resistance of the slow pasteurization and fast pasteurization for Lb,pla and Lb.GG for control and bacteria coated one layer and bacteria coated two layer at 63°C/ 30 minutes and 72°C/ 15 seconds. The results indicate that the Probiotic coated two layer are more resistant to pasteurization temperatures at 63°C/ 30 minutes and 72°C/ 15 seconds than the Probiotic coated one layer. While the results of the control follow a significant reduction for viability of cell toward pasteurization temperatures. The percentage of reduction of probiotics coated two layer was 19.78 and 15.54% for the slow pasteurization, 21.05 and 17.04% for the fast pasteurization. The percentage of reduction for probiotic coated one layer (Alginate) was 32.55 and 25.81% for the slow pasteurization, 30.4 and 26.7% for the fast pasteurization, while reduction of the control percentage was 74.43 and 72.16% for the slow pasteurization, 72.62 and 70.34% for the fast pasteurization to Lb.Pla and Lb.GG respectively.
Background
Respiratory tract aspergillosis is a pulmonary disease cause by aspergillus species which are opportunistic fungi that mainly infect immuno-compromised patients .
Objective(s)
The present study aimed to detect the frequency of pulmonary aspergillosis among clinically suspected and under follow up tuberculosis patients conducted at Tropical Diseases Teaching Hospital, Omdurman, Khartoum State , Sudan during the period from December 2019 to November 2020.
Materials and Methods
One hundred and fifty sputum samples were collected from suspected cases of pulmonary tuberculosis and under follow up tuberculosis patients. All specimens were examined using 20% KOH and cultured on two
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