The study included the collection of samples of raw cow milk to isolate Leuconostoc bacteria, samples were sub cultured on De-Man Rogosa Sharpe-Vancomycin medium, the pure colonies were selected and subjected to the cultural and microscopically tests, according to that 25 cocci bacterial isolates were obtained, then isolates were subjected to biochemical tests. Result of tests showed that 12 isolates belong to the genus Leuconostoc out of 25 cocci bacterial isolates, Vitek2 system was used as a supplementary step. Results of final identification showed that 3 sub species were obtained included Leuconostoc mesenteroides ssp. cremoris 9 out of 12 isolates, while it was 2 isolates of Leuconostoc mesenteroides ssp. mesenteroides and one isolate Leuconostoc mesenteroides ssp. dextranicum. The study included the collection of 15 isolate of diarrhea causative bacteria which subjected to cultural and microscopically, biochemical tests and Vitek 2 system, about the results of identification, 9 isolates out of 15 isolates belonged to Escherichia coli,3 isolates of Shigellagroup,2 isolates of Salmonella ser. paratyphi A and one isolate of Vibrio cholerae, the selected isolates were tested to Antibiotic sensitivity test against 11 antibiotics, the results showed that all isolates were resistant to Ampicillin and sensitive to Imipeneme. Screening of L. mesenteroides ssp. cremoris isolates was done to select the more efficient isolate producing bacteriocin by using well diffusion method, the result showed that L. mesenteroides ssp. cremoris (Lc3) and L. mesenteroides ssp. cremoris (Lc4) isolates gave the highest inhibition against E. coli, Shigella group, Salmonella ser. paratyphi A and Vibrio cholerae, the diameters were 13, 12, 12 and 12 mm respectively for the L. mesenteroides ssp. cremoris (Lc3) isolate, while the L. mesenteroides ssp. cremoris (Lc4) isolate diameters were 13, 12, 12 and 11mm respectively. The synergistic effect between bacteriocin of both isolates L. mesenteroides ssp. cremoris (Lc3) and L. mesenteroides ssp. cremoris (Lc4) with antibiotics was studied against multidrug resistant diarrhea causative bacteria. Synergistic effect between bacteriocin of both isolates with antibiotics was observed. The activity of antibiotics with bacteriocin was increased, like Cefixime, Tetracycline, Trimethoprim-sulfamethoxazole, Ampicillin antibiotics against E. coli, and Cefotaxime, Ceftriaxone, Trimethoprim-sulfamethoxazole, Cefixime antibiotics against Shigella group, and Trimethoprim-sulfamethoxazole antibiotic against Salmonella ser. paratyphi A and Ampicillin, Trimethoprim-sulfamethoxazole, Cefixime antibiotics against Vibrio cholerae.
Background: Any child with Down's syndrome does not develop in the same manner as normal child. Therefore, the child should not be viewed as being like everyone else. Developmental enamel defects in primary teeth have been found at least twice as frequently in disabled children as in control children. Down's syndrome consumed protein more than the recommended daily allowance compared to other disabled groups. Therefore, the aim of this study was to investigate developmental defects of enamel and their relations to nutrient intake among Down's syndrome children in comparison to normal children. Materials and Methods: A sample consisted of fifty institutionalized Down's syndrome children (study group) and 50 normal children (control group)
... Show MoreA case–control study (80 patients with chronic hepatitis B virus [HBV] infection and 96 controls) was performed to evaluate the association of an IL12A gene variant (rs582537 A/C/G) with HBV infection. Allele G showed a signifcantly lower frequency in patients compared to controls (31.2 vs. 46.9%; probability [p]=0.009; corrected p [pc]=0.027) and was associated with a lower risk of HBV infection (odds ratio [OR]=0.49; 95% confdence interval [CI]=0.29–0.83). A similar lower risk was associated with genotypes CG (17.5 vs. 29.2; OR=0.25; 95% CI=0.08–0.81; p=0.02) and GG (10.0 vs. 16.7; OR=0.25; 95% CI=0.07–0.91; p=0.036), but the pc value was not signifcant (0.12 and 0.126, respec‑ tively). Serum IL35 levels showed signifcant difere
... Show MoreIn addition to their high quantities of active chemicals, medicinal plants are well-known for their pharmacological qualities, which include immunological modulation. T Consequently, this study aimed to examine the effects of Avena sativa and Glycyrrhiza glabra leaf extracts on immunological responses as measured by blood cytokine and liver enzyme levels. The phytochemical analysis of Avena sativa crude leaf extracts revealed the presence of alkaloids,flavonoids, tannins, phenolic compounds, and saponins but the absence of resins and violet oils. On the other hand, violet oils, flavonoids, tannins, saponins, and glycosides were detected in significant concentration in Glycyrrhiza glabra ethanolic extract, although resins and phenolic compou
... Show MoreObjectives: To determine the effectiveness of the educational program on nursing staff knowledge about infection control measures at the Intensive Care Unit in Al-Diwaniya Teaching Hospital.
Methodology: A pre-experimental design (one group design: pre-test and post-test) was used. This study was conducted in Al-Diwaniya Teaching Hospital for the period from ( 20th February to 5th March, 2020) on a non-probability (purposive) sample consisting of (25 nurses) working in ICU. A questionnaire was built as a data collection tool and consisted of two parts:
First part: The demographic characteristics of the nursing staff (age, gender, level of education, years of experien
... Show MoreContinuous turbidimetric analysis (CTA) for a distinctive analytical application by employing a homemade analyser (NAG Dual & Solo 0-180°) which contained two consecutive detection zones (measuring cells 1 & 2) is described. The analyser works based on light-emitting diodes as a light source and a set of solar cells as a light detector for turbidity measurements without needing further fibres or lenses. Formation of a turbid precipitated product with yellow colour due to the reaction between the warfarin and the precipitation reagent (Potassium dichromate) is what the developed method is based on. The CTA method was applied to determine the warfarin in pure form and pharmaceu