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STUDYING THE EFFECT OF Proteinase K AND α-Amylase ON INHIBITION OF Staphylococcus aureus AND Klebsiella pneumoniae BIOFILM ISOLATE FROM URINARY TRACT INFECTION.: STUDYING THE EFFECT OF Proteinase K AND α-Amylase ON INHIBITION OF Staphylococcus aureus AND Klebsiella pneumoniae BIOFILM ISOLATE FROM URINARY TRACT INFECTION.
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The current study aimed to investigate the viability of biofilm formation klebsilla pneumoniae and Staphylococcus aureus. 440 urine samples were collected from patients suffering from urinary tract infection (UTI) from those who were admitted and visitors to Al-Ramadi Teaching Hospital, Al-Yarmouk Teaching Hospital, Al-Ramadi Teaching Hospital for women and children and , Teaching Laboratories in the Medical City for both genders for a period extended from 5 July, 2017 to 10 October, 2017. Samples were diagnosed by culturing them on a selective media and by biochemical testes , also, diagnosis was ensured by using VITEK-2 compact system. Results showed that K.pneumoniae isolation ratio was 17.1%(68) and S.aureus ratio was 13.1%(52). Their sensitivity was tested against 10 antibiotics, isolates showed uneven resistance according to the type of bacteria. Bacterial biofilm formation viability was detected by qualitative and quantitative methods, isolates per bacteria. Artificial material was used proteinase K and α-Amylase with triple concentrations to each enzyme 33,100,250 µg/ml and 20,100,200 mg/ml respectively, with biofilm inhibition of testing bacteria by precision calibration dishes method. Result showed noticeable decreasing in biofilm formation after enzymes addition in comparison with its formation before enzymes addition, where high decreasing ratio of biofilm according to proteinase K was at concentration 33µg/ml with 95% ratio, while less inhibition to K.pneumoniae, the same concentration showed inhibition viability at 93% and less inhibition viability at 259 µg/ml with 61%. Also, regarding to α-Amylase showed a high inhibition activity at 95%with concentration 100 mg/ml to S.aureus and less inhibition ratio was found at 20 mg/ml with 87%. While to K.pneumoniae , the triple concentrations showed inhibition activity ranged between 36-77%. Statistical analysis showed presence of significant variation between each enzyme concentration to both types of bacteria, also presence of significant variation among different concentration to each bacterial type at (P≤0.01).

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Publication Date
Sat Sep 30 2023
Journal Name
Lark
مسؤولية مجلس إدارة المصرف في إطار السياسة الائتمانية
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Publication Date
Wed Oct 05 2016
Journal Name
Al-academy
Holy representations in European religious duties (1200- 1600)
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The representations of the Holy in Christian painting Art represents a penalty of eternal relationship between any human society and sacred representations and forms that he belives in what is characterized by its clear tendency to knowledge and aclear focus on the spiritual experience that make up the human relationship with holy spirit and essence. Search has ment to study the forms of its presence in the art work of the middle ages.Research involved four shapters .first chapter includes research problem which centered around the following question;what is holy ? whats its represetations in Christian painting? And the importance and need of the study .and the goal of research and definitaions of the terms of research.Second chapter con

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Publication Date
Sun Oct 21 2018
Journal Name
Journal Of Planner And Development
التمكين الإقتصادي للمرأة الريفية ودورها في التنمية المستدامة
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Publication Date
Tue Jan 08 2019
Journal Name
Arab Science Heritage Journal
إمارة الحج في عهد الخليفة أبي العباس السفاح
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يعد موضوع إمارة الحج من الموضوعات المهمة في التاريخ الإسلامي، فهو يتعلق بأحد أركان الإسلام، ومسؤولية إقامة الحج وإمارته كبيرة، ومن الأسباب التي كانت وراء اختيار عنوان هذا البحث " إمارة الحج في عهد الخليفة أبي العباس السفاح"، أن الموضوع يقع ضمن دائرة اهتمامنا في متابعة إمارة الحج في الدولة العربية الإسلامية، لاسيما إننا بحثنا الموضوع ذاته في دراسات سابقة . وقد تناولنا إمارة الحج حتى نهاية الدولة الأموية

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Publication Date
Sun Nov 04 2018
Journal Name
Arab Science Heritage Journal
المحاريب المحاريب العراقية ، تاريخها ، انواعها في العصر العباسي
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The mihrab is an architectural element created by the Muslims for the necessities of which the direction of Qibla is set in the mosques, and the front stands in it during the prayer so as to provide a complete description of the Muslims inside the chapel. The mihrab has a third benefit in amplifying the voice during the time of prayer, Peace be upon him) and the Mosque of Damascus, the Mosque of Kairouan, and the Mosque of Amr Balfstat?.

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Publication Date
Sun Jan 01 2012
Journal Name
Political Sciences Journal
دور الحكومات في بناء الدولة ضمن اطار الدستور
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دور الحكومات في بناء الدولة ضمن اطار الدستور

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Publication Date
Sun Jan 02 2005
Journal Name
Political Sciences Journal
واقع ومستقبل الصراعات الدولية والاقليمية في البحر الاحمر
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واقع ومستقبل الصراعات الدولية والاقليمية في البحر الاحمر

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Publication Date
Wed Jan 02 2008
Journal Name
Political Sciences Journal
اثر المحددات الخارجية والداخلية في بناء االدولة لعراقية
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اثر المحددات الخارجية والداخلية في بناء الدولة العراقية

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Publication Date
Sun Feb 10 2019
Journal Name
Journal Of The College Of Education For Women
Social Satire in Harold Pinter’s Celebration
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Harold Pinter was one of the most important dramatists in the modern and the
contemporary age. Pinter presented his own dramatic realm in which he turns the theatre into
a moral institution. He employed the elements of several schools of drama and manipulated
them in order to deliver his ethical messages emphasizing the necessity to regain and renew
the world's morality in order to face the political and social injustices committed against
humanity. His writings developed over more than fifty years in style, techniques and his
interests. However, his main concern was his social and political satire.

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Publication Date
Sat Jan 01 2011
Journal Name
Political Sciences Journal
مستقبل الايديولوجيا واليوتوبيا في الفكر السياسي الغربي الحديث
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مستقبل الايديولوجيا واليوتوبيا في الفكر السياسي الغربي الحديث

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