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Basal cell markers:34BE12 and p63, improving detection of basal cells in atypical prostatic lesions
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Background: The diagnosis of prostatic pathology may be of challenging , as some  difficult and suspected, atypical  cases may lack basal cell layer by routine H&E sections . Antibodies against 34BE12(HMW-CK) and p63 aid the diagnosis of such cases , to distinguish benign from  malignant prostatic lesions.

Objective: to identify basal cells in atypical prostatic lesions ,and distinguish benign from malignant prostatic lesions.

Type of the study:  A retro-spective  study.

Methods:  115cases of  paraffin embedded prostatic tissue blocks ,diagnosed as : 76 cases were benign prostatic hyperplasia( BPH) , 9 cases were  high grade –prostatic intraepithelial neoplasia (HG-PIN) , and 30 cases were prostatic carcinoma(PCa) .Sections from each blocks were prepared for immunostaining with 34BE12 and p63.

Results : basal cells were detected in cases of BPH , and HG-PIN , and absent  in all cases of prostatic carcinoma ,using basal cell markers . Negative benign glands(>2) were found in 71.6% and 38.2%  for   BPH  and 57.1% and 55.6% for HG-PIN immunostained  with high molecular weight cytokeratin (34BE12) and p63 , respectively, and significantly reduced to 9.0% and 11.1% for BPH and HG-PIN, respectively  with combined using of both markers .Conclusion : Combination of both   basal cell markers (34BE12 , p63) improving basal cell detection in atypical ,suspected prostatic lesions and distinguish benign from malignant lesions.

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Publication Date
Thu May 31 2018
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Seroprevalence of Toxoplasmosis Antibodies among Diabetes Mellitus Patients and Assessment some Biochemical Markers
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         Toxoplasma gondii is an protozoan intracellular coccidian protozoan parasite. Latent toxoplasmosis threat to immunocompetent individuals. Diabetic patients are more susceptible to infect with toxoplasmosis due to their low level of immunity response. The purpose of this research is to define the association between toxoplasmosis and diabetes mellitus and detection serum levels of chemokines (monocyte chemoattractant protien-1 and transforming growth factor-β) in diabetic patients infected with toxoplasmosis. Serum samples were collected from 120 diabetic patients and 50 healthy individuals as a control group  from the Imamein Kadhimein Medical City in Baghdad. In order&nbs

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Publication Date
Thu Jun 29 2023
Journal Name
Iraqi Journal Of Chemical And Petroleum Engineering
Synthesis of Novel Porphyrin Derivatives and Investigate their Application in Sensitized Solar Cells
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Solar energy has significant advantages compared to conventional sources such as coal and natural gas, including no emissions, no need for fuel, and the potential for installation in a wide range of locations with access to sunlight. In this investigation, heterocyclic derivatives were synthesized from several porphyrin derivatives (4,4',4",4"'-(porphyrin-5,10,15,20-tetrayl) tetra benzoic acid) compound (3), obtained by reaction Pyrrole with 4-formyl benzoic acid. Subsequently, porphyrin derivative-component amides 5a, 5b, and 5c were produced by reacting compound (3) with amine derivatives at a 1:4 molar ratio. These derivatives exhibited varying sensitivities for utilization in solar cells, with compound 5a displaying the highest power

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Publication Date
Fri Sep 27 2019
Journal Name
Journal Of Baghdad College Of Dentistry
Clinical Assessment and Cytomorphometric Analysis of Buccal Mucosal Cells in Behçet’s Disease Patients
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Background: Behçet’s disease (BD) is a disorder of systemic inflammatory condition. Its important features are represented by recurrent oral, genital ulcerations and eye lesions. Aims. The purpose of the current study was to evaluate and compare cytological changes using morphometric analysis of the exfoliated buccal mucosal cells in Behçet’s disease patients and healthy controls, and to evaluate the clinical characteristics of Behçet’s disease. Methods. Twenty five Behçet’s disease patients have been compared to 25 healthy volunteers as a control group. Papanicolaou stain was used for staining the smears taken from buccal epithelial cells to be analyzed cytomorphometrically. The image analysis software has been used to

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Publication Date
Fri Jul 28 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Morphological and Growth Properties of Albino Rat Bone Marrow Stromal Cells In Vitro
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   The potential use of bone marrow stromal cells as a cellular therapy for chronic diseases relies on the ability of the cell to replicate extensively in vitro.For this reason the present study investigated the replication lifespan and examined the growth properties of albino rats mesenchymal stem cells(MSCS)in vitro. To establish an in vitro system for isolating and culturing the  MSCs of albino rats and to provide research data for its further application,the bone marrow (BM)was collected from young male rats and separated by gradient centrifugation.Then, the mononuclear cells(MNCs) were retrieved from the buffy layer and cultured in Modified Eagle,s Medium (MEM) supplemented with 10%Fetal Calf Serum (FCS)and incubation

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Publication Date
Thu Apr 01 2021
Journal Name
Annals Of The Romanian Society For Cell Biology
Evaluation of Nesfatin – 1 and Other Biochemical Markers in diabetic Neuropathy Iraqi patients before and after treatment with tegretol
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Diabetic neuropathy is a form of nerve damage that can occur in people who have diabetes. High blood sugar (glucose) induced nerve damage in every part of the body. The nerves in the legs and feet were the most frequently affected. The extent to which a diabetic patient's body is impaired is calculated by the degree of nervosa harm.The purpose of this present study is estimation BMI,IL-10 , nesfatin-1 and HS-CRP in Iraqi DN patients before and after treatment via tegretol as well as it is the first study sheds light on the relationship between Nesfatin -1 and other parameters ( BMI,IL-10 and HS-CRP) also predication of Nesfatin-1 as a newly biomarker in patients with diabetic neuropathy. The present study consist of from 30 cohort G1 as hea

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Publication Date
Wed Nov 01 2023
Journal Name
Journal Of Medicine And Life
Neuroprotective effects of daidzein against ifosfamide-induced neurotoxicity in male rats: role of selected inflammatory and apoptotic markers
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Ifosfamide (IFO), an alkylating chemotherapy agent, is known for its association with neurotoxicity and encephalopathy. This trial was designed to evaluate the protective action of daidzein (DZN) against IFO-induced neurotoxicity in male rats by determining the difference in certain inflammatory and apoptotic markers in the brain tissue of rats. Twenty-eight Wistar rats, weighing 120-150 g, were divided into four groups of seven rats: Group 1 (Control) received no treatment; Group 2 was orally administered DZN (100 mg/kg/day) for seven days; Group 3 received a single intraperitoneal (IP) dose of IFO (500 mg/kg); Group 4 received oral DZN (100 mg/kg/day) for one week prior to a single IP dose of IFO on the seventh day. Twenty-four hours post

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Publication Date
Mon May 15 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Datura stramoium Extract Inhibits Mitosis in Cancer Cells
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  The aim of this study was to evaluate the anti mitotic activity of ether extract prepared from Datura stramoium plant on cancer cells.  Recently oncology studies are directed toward substances which act somehow to induce apoptosis or to prevent abnormal dividing of cell, that may provide therapeutic strategies for the treatment of cancer. Thus, many research on various types of plants such as fruits, vegetables, other editable plants and toxic plants have been done in vitro and in vivo. But, few studies on Datura plant were occurred and there was no previous study dealing with its effect on proliferative activity of cancer cells.  In current study, two types of common malignant tumor were tested in vitro. These were mamm

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Publication Date
Fri Dec 30 2011
Journal Name
Al-kindy College Medical Journal
T-Cells Proliferation and Serum Cytokine levels in Type 1 Diabetic Children
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Background: There is plenty of evidence
suggesting that involvement of several groups of
viruses in the development and / or acceleration of
Type 1 Diabetes Mellitus (T1DM).
Objective: To analyze the T- cell proliferation in
the presence of Coxsackie virus B5 (CVB5), Polio
and Adenovirus antigens in addition to assessment
of Interferon- gamma (IFN-γ), Interleukins (IL-10
and IL-6).
Methods: In 60 Iraqi T1DM children with recent
onset of T1DM, Lymphocyte proliferation was
analyzed using Methylthiazol tetrazolium (MTT)
assay by culturing Peripheral Blood Lymphocytes
(PBLs) with Coxsackie Virus B5 (CVB5),
Adenovirus, and Polio vaccine. Serum Interferon-γ,
IL-10 and IL-6 were quantified by sandw

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Publication Date
Sun Oct 15 2023
Journal Name
Sumer 3
Treatment of shallow and deep white spot lesions with three different mouthwashes evaluated by laser fluorescence (an in vitro study)
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This research aims to find how three different types of mouthwashes affect the depth of artificial white spot lesions. Teeth with various depths of white spot lesions were immersed in either splat mouthwash, Biorepair mouthwash, Sensodyne mouthwash, or artificial saliva (control)twice daily for one minute for 4 weeks and 8 weeks at 37°C. After this immersion procedure, lesion depth was measured using a diagnosed pen score. A one-way analysis of variance, Dunnett T3 and Tukey's post hoc α = .05 were used to analyze the testing data. Splat mouthwash enhanced the WSL remineralization and made the lowest ΔF compared with other mouthwashes in shallow and deep enamel after 4 and 8 weeks of treatment. In the repair groups, after 4 weeks

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Publication Date
Fri Dec 01 2023
Journal Name
Experimental And Applied Biomedical Research (eabr)
Correlation Between Ultrasound BI-Rads 4 Breast Lesions and Fine Needle Cytology Categories in a Sample of Iraqi Female Patients
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Breast cancer is the most common malignancy in female and the most registered cause of women’s mortality worldwide. BI-RADS 4 breast lesions are associated with an exceptionally high rate of benign breast pathology and breast cancer, so BI-RADS 4 is subdivided into 4A, 4B and 4C to standardize the risk estimation of breast lesions. The aim of the study: to evaluate the correlation between BI-RADS 4 subdivisions 4A, 4B & 4C and the categories of reporting FNA cytology results. A case series study was conducted in the Oncology Teaching Hospital in Baghdad from September 2018 to September 2019. Included patients had suspicious breast findings and given BI-RADS 4 (4A, 4B, or 4C) in the radiological report accordingly. Fine needle aspirati

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