In this research, the structural and optical measurements were made on the Zinc oxide (ZnO) films prepared by two methods once by using chemical spray pyrolysis technique, and another by using thermal evaporation technique before and after irradiation by Gamma –Ray (γ – rays) from source type (Cs 137) with an energy (0.611)MeV as a function of gamma dose (0.15,0.3 and 0.45) Gy. The thickness of all films prepared by two method was about (300 ± 50) nm. XRD is used to characterize the structural properties, the results demonstrated that all samples prepared by two method before and after irradiation have polycrystalline structure with a preferred orientation (002).Also it showed that the structural properties are weakly dependent on the gamma dose. The optical measurement shows that all ZnO films prepared by two method have a direct energy gap, and they in general decrease with the increase of Gamma dose while the optical constant such as absorption coefficient, refractive index, extinction coefficient, real and imaginary parts of the dielectric constant and optical conductivity showed an opposite trend, these values increase with the increase of irradiation dose. As well as all optical properties for the samples prepared by thermal evaporation technique is higher than the samples prepared by chemical spray pyrolysis technique.
Lipopolysaccharide (LPS) of Campylobacter coli was extracted by using
digestive enzyme and hot phenol method. The effect of LPS on lymphocyte
transform was studies by lymphocyte transformation index for twenty blood samples
were collected from apparently healthy control.
The results were showed significant differences (P< 0.05) between samples which
treatrd with phytohemagglutnin PHA (66.1 ± 0.6) and the samples which treated
with LPS of C. coli (74.2 ± 0.8) when compared with control, this lead to suggest
that the LPS extracted from C. coli may play a role as a mitogen to transformed
lymphocytes.
Fifteen blood samples were collected from healthy males and females (6 males &
9 females), average age (21-34 years) in heparinized sterile tubes. The extracellular
protease was extracted from a clinical isolate of Serratia marcescens that was
isolated from a patient suffering from urinary tract infection taken from the Central
Health Laboratory. The extracted protease was purified partial by two steps,
precipitation with 30-55% saturation of ammonium sulfate following with dialysis
and ion exchange chromatography DEAE-cellulose. The protease concentration was
0.15 mg/ml. Two concentration 0.258g/ml and 0.58/ml of protease were prepared
and applied in current study. Lymphocyte transformation test using whole b
Nanoparticles are a special group of materials with unique features and extensive applications in diverse fields. The use of nanoparticles of some metals is a viable solution to stop infectious diseases due to the antimicrobial properties of these nanoparticles. The present work demonstrates the effect of silver nanoparticles (AgNPs) on the antibacterial activity of four different antibiotics (amoxicillin, ceftriaxone, chloramphenicol, and penicillin) against eleven Gram-positive and Gram-negative isolates. Disk diffusion method was used to determine the antibacterial activity of various classes of antibiotics in the absence and presence of sub-inhibitory silver nanoparticles of concentration (80 microgram/ml). A synergistic effect was o
... Show MoreThe ability of microorganisms to attach to living and non-living surfaces and create a biofilm is the cause of numerous long-lasting illnesses, as well as their strong resistance to drugs. Bacterial biofilms consist of intricate assemblies of immobile bacteria. These are located in an extracellular matrix and adhere to various surfaces for a long period. The present study evaluated the antibacterial effectiveness of Plantago major extract against Staphylococcus aureus biofilm. The specimens analyzed in this investigation were skin infections of clinical origin. The current study was not previously studied, particularly in terms of S. aureus biofilm breakdown and inhibition. The disc diffusion method was used to test the antimicrobial activi
... Show MoreThe ability of microorganisms to attach to living and non-living surfaces and create a biofilm is the cause of numerous long-lasting illnesses, as well as their strong resistance to drugs. Bacterial biofilms consist of intricate assemblies of immobile bacteria. These are located in an extracellular matrix and adhere to various surfaces for a long period. The present study evaluated the antibacterial effectiveness of Plantago major extract against Staphylococcus aureus biofilm. The specimens analyzed in this investigation were skin infections of clinical origin. The current study was not previously studied, particularly in terms of S. aureus biofilm breakdown and inhibition. The disc diffusion method was used to test the antimicrobial activi
... Show MoreForty eight isolates (41.02%) were obtained from 117 wound and burn samples. The isolates that showed high resistance for both antibiotic was two only that represent 4,1% from all isolates. The result of PCR product electrophoresis was referred that the gene is VIM gene. Lactose and raffinose showed double increasing in diameter of inhibition zone of imipenem with 1% that mean showed highest susceptibility that decreased with the concentration increasing, the same result were with meropenem. But no effect were detected on meropenem inhibition zone diameter. Mannose have no effect on the resistance in 1%, 3% and 7%. Results showed that only three case that increase the expression of gene, they were lactose at 1% concentration that increased
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Between October and December 2018, 27wounds and burn swab specimens were collected by laboratories at Al-Yarmook hospital, and cultured on Mannitol salt agar. the isolate was subjected to Nd: YAG laser in different power (400mJ, 500mJ, 800mJ and 900mJ). In general the laser showed effect on bacterial growth that reach to complete killing, the statistical analysis showed that there is weak correlation between laser at 400mJ with killed percentage. While in 500mJ its exhibit complete correlation with killing percentage, this correlation was decreased with increasing in power to 800mJ and 900Mj.
The current study aimed to evaluate the effect of the heavy metals copper, cadmium and cobalt when added individually, in combination and in combination on the growth and reproduction of the aquatic fungus Saprolegnia hypogyna.
Background: All diseases concerning bone destruction such as osteoporosis and periodontal diseases share common pattern in which the osteoclast cells are absolutely responsible for bone resorption that occurred when osteoclast activity exceeds osteoblast activity. Osteoprotegrin (OPG) considered as novel soluble decoy receptor known as “bone protector†since it prevents extreme bone resorption through inhibition of differentiation and activity of osteoclast by competing for binding site. It binds to receptor activator of nuclear factor kappa-B ligand (RANKL) and prevent its interaction with receptor activator of nuclear factor kappa-B (RANK), thus inhibits osteoclast formation. TNF-α is a pro-inflammatory cytokines having
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The experiment for the growing season. 2015-2016 to study the effect of gibberellic acid at concentrations (0 and 50)mgL-1 and BL at five concentrations (0, 0.5 ,1 ,2 and 3)mg.L-1 and their interaction on some chemical characteristics for Dill plant . The experiment was designed according to Randomized Complete Block Design (RCBD) with three replicates per treatment, using less significant difference at the level of probability (0.05) , the results showed the following:- 1- The effect of brassinolide with it,s concentrations led to obtain on a significant increase in all the studied characteristics, so the superiority of the concentration of 2 mg.L -1 of brassinolide in each of nitrogen, potassium and protein, And the superi
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