A direct solvent extraction was used for the determination of phenolic compounds from Teucrium polium . Methanol, ethanol, iso-propanol, butanol and D.W were used as an extractants and study the affecting extraction factors including : the type and solvent concentration, temperature, extraction time and number of batch extraction, by using Folin-ciocalteu method to determine the Total Phenolic Content (TPC) and measurement of the absorbance at λmax 765nm. The accuracy and precision of this method were determined by preparing laboratory samples of Gallic acid , the results showed relative errors ranging from ±0.5 – 1.49 % and the standard deviation equal to 1.294 and the relative standard deviation did not exceed 0.71 %, the standard deviation of the mean was 0.647 and relative standard deviation of the mean was equal to 0.353% . The results showed that the highest extraction was obtained equals to 97% by methanol followed by ethanol and D.W., especially when the extraction takes place with 100% concentration , at 8 hr extraction time at room temperature with two extraction batches. This method was applied under the optimized experimental condition found in this work for two Iraqi local life samples from Kut and Dehouk , the total phenolic content measurements were found to be equal to 183.250 and 236.991 mg/ g of dry Teucrium polium plant stem and 129.214 and 101.022 mg/g of dry Teucrium polium plant for Kut and Dehouk samples respectively .
