The Present investigation includes the isolation and identification of Pseudomonas aeruginosa for different cases of hospital contamination from 1/ 6/2003 to 30/9/2004, the identification of bacteria depended on morphological , cultural and biochemical characters, 37 of isolates were diagnosed from 70 smears from wounds and burns beside 25 isolates were identified from 200 smears taken from operation theater and hospital wards including the floors , walls , sources of light and operation equipment the sensitivity of all isolates to antibiotic were done , which exhibited complete sensitivity to Ciprofloxacin , Ceftraixon, Tobromycin and Gentamysin ,while they were complete resist to Amoxcillin , Tetracyclin , Nitrofurantion , Clindamycin C

The present study aims to evaluate the synergistic activity of nicotinic acid (NIC) with the Imipenem (IMI) as an anti-biofilm for clinical isolated Pseudomonas aeruginosa. The values of minimum inhibitor concentration (MICs) for IMI and NIC (Separately) against P. aeruginosa were (16) ug/mL and (8) ug/ml respectively. Whereas, the concentration of NIC with IMI (as combined) for biofilm inhibition was  1 ug/ml for NIC and 4 ug/ml for IMI. The combining of NIC with IMI showed synergistic efficacy against formation of bacterial biofilm (at MIC levels). These     results provide a conclusion that NIC combined with IMI is can be considered as a successful prospective treatment against the biofilm pr

Petroleum is one of the most important substances consumed by man at present times, a major energy source in this century, petroleum oils can cause environmental pollution during various stages of production, transportation, refining and use, petroleum hydrocarbons pollutions ranging from soil, ground water to marine environment, become an inevitable problem in the modern life, current study focused on bioremediation process of hydrocarbons contaminants that remaining in the bottom of gas cylinders and discharged to the soil. Twenty-four bacterial isolates were isolated from contaminated soils all of them gram negative bacteria, bacterial isolates screening to investigate the ability of biodegradation of hydrocarbons, these isolates inocula

     The study was aimed at inhibiting the protease produced by Pseudomonas aeruginosa using an 80% alcoholic extract of Conocarpus lancifolius leaves. A total of 146 isolates of P. aeruginosa that were isolated and identified by microscopic and biochemical tests were 51 isolates submitted to primary and secondary screening techniques in order to choose the qualified P. aeruginosa isolate for protease synthesis. Among these isolates, forty-seven isolates showed hydrolysis zones on skim milk media (primary screening); six isolates were chosen for secondary screening. The result revealed that P. aeruginosa P51 had the highest ability to produce the enzyme, with a specific activity of 15.9 U/

A total of 172 clinical were obtained over 6 months. Klebsiella spp. was detected in 58 (33.7%) samples with a high percentage 29 (50%) in urine in female and low percentage 1(1.7%) in pus and burn swabs in male, and the vaginal swab was 1(1.7%). The female to male ratio was 3.1:1. PCR detection showed that 51(87.93%) out of 58 produce 108 bp. product with rpoB specific primer that represented K. pneumonia. Whereas 7(12.07%) showed PCR product with 343 bp by K. oxytoca specific primer (peh X), furthermore, the sequences of two selected isolates showed that the species related to K. oxytoca strain CAV1335, and to K. oxytoca strain CAV1374. Five selected isolates were re-tested by the gyr A primer, all were showed specific band product wit

        Peroxidase is a class of oxidation-reduction reaction enzyme that is useful for accelerating many oxidative reactions that protect cells from the harmful effects of free radicals. Peroxidase is found in many common sources like plants, animals and microbes and have extensive uses in numerous industries such as industrial, medical and food processing. In this study, P. aeruginosa was harvested to utilize and study its peroxidases. P. aeruginosa was isolated from a burn patient, and the isolate was verified as P. aeruginosa using staining techniques, biochemical assay, morphological, and a sensitivity test. The gram stain and biochemical test result show rod pink gram-ne

This study aims at detecting the differences in genotyping of coding region fusA gene in clinical isolates of Acinetobacter baumannii from Baghdad, Iraq. Collected two hundred clinical samples (50 samples from urine, 50 samples from wound, 50 samples from sputum and 50 samples from otitis infections). Laboratory diagnosis for bacterial isolates carried out by some biochemical tests and confirmed by using VITEK- 2 compact system. The results appeared that twenty isolates of Acinetobacter baumannii in all these samples. Genotyping study was performed of coding region fusA gene of the extracted genome of all bacterial isolates and used specific primers in achieved amplification process of this target gene. DNA sequencing of this gene and alig

     The presence of heavy metal in environment associated with several health problems. The clean up environment from lead (Pb) and Nickel (Ni) represent major challenges. In his study, planktonic and immobilized bacteria were used to purify the water from Pb and Ni in Lab. In the present study, three bacterial isolates of Staphylococcus aureus (isolated from wound swaps), Pseudomonas aeruginosa (isolated from wound swaps) and Pantoea (isolated from urine samples) and identified using biochemical methods to check their ability to biosorb Pb and Ni. Ten PPM of Pb and Ni were added to the deionized distilled water and 107 c.f.u. of planktonic bacteria were used to biosorpe Pb and Ni. Similar experiment was repeated but

The purpose of this study to synthesize and characterize silver nanoparticles using phenolic compounds obtained from Camellia sinensis, to test the antibacterial properties of biosynthesized nanoparticles on the formation of biofilms in multidrug-resistant Pseudomonas aeruginosa. Ten isolates of P. aeruginosa were obtained from the Genetic Engineering and Biotechnology Institute laboratories of the University of Baghdad. By using the VITEK-2 system and culturing the isolates on cetrimide agar, the diagnosis was confirmed. Camellia sinensis silver nanoparticles (CAgNPs) were created using an extract of the plant's aqueous and methanolic leaves. Based on the results of the nanoparticle synthesis, spherical nanoparticles that may be single or