The cellular and molecular toxicity effect of Aloe Vera crude extract on meristem cells of onion (Allium cepa) roots were studied . Different concentrations of this crude extract 20% ,10% ,5% ,2% ( and 40%) were used at different periods ; 24 ,48 and 72 hour . Number of chromosomal aberrations and the mitotic index was calculated . In addition, the genetic effects were observed by using randomly amplified polymorphic DNA ( RAPD ) technique . The results showed that the gel crud extract of Aloe vera had inhibition effects on the growth of the onion roots with 10% EC50 value. Significant effects of this gel crud extract were also observed on the chromosomal aberration and mitotic index by decreasing of divided cell rates. The toxic effect of 20% concentration of gel crud extract was decreased the mitotic index under 50% , in the comparison with control sample . Chromosomal aberration were also observed in divided cells of onion roots, which exposed to different concentrations at different periods . The higher frequent type of this chromosomal aberrations , which observed are stickiness , disturbed , bridge , c-mitosis , vagrant and star telophase . The result of RAPD technique reveled differences in number of DNA bands and their molecular weights of the treated samples , which exposed to high concentration of crud extract in comparison with the control sample . The result of the genetic stability ratio (% GTS), also showed that Aloe vera crud extract have genetic toxicity at 40% concentration.
Asphaltene is one of the fractions of the crude oil which is soluble in aromatics such as benzene or toluene and insoluble in alkane such as n-heptane, n-pentane or petroleum ether (mixture of alkane compounds). Asphaltene precipitation is one of the most common problems that sometimes occurs in both oil recovery and refinery processes as a result of changing in pressure, oil composition, or temperature. Therefore the stability of asphaltene in the crude oil must be studied to show the tendency of it for precipitating asphaltene to prevent it (Asphaltene precipitation and deposition problem) and eliminate the burden of high treatment costs.
In the present study, saturate, aromatic, resin and asphaltene (SAR
... Show MoreFormation of emulsions during oil production is a costly problem, and decreased water content in emulsions leads to increases productivity and reduces the potential for pipeline corrosion and equipment used. The chemical demulsification process of crude oil emulsions is one of the methods used for reducing water content. The demulsifier presence causes the film layer between water droplets and the crude oil emulsion that to become unstable, leading to the accelerated of water coalescence. This research was performed to study the performance of a chemical demulsifier Chimec2439 (commercial) a blend of non-ionic oil-soluble surfactants. The crude oils used in these experiments were Basrah and Kirkuk Iraqi crude oil. These
... Show MoreThis study reflected on the relationship between contactin associated protein-like
2 gene (CNTNAP2) and autism spectrum disorders. The study includes forty autistic
patients and forty non autistic children as control groups (twenty unaffected sibling
and twenty unrelated children). DNA was extracted from Blood samples for
molecular detection of CNTNAP2 mutations associated with ASDs by using
Polymerase Chain Reaction (PCR) technique and sequencing analysis. PCR reaction
was performed to amplify exon 20 of CNTNAP2 gene. The PCR results revealed that
identical bands related to exon 20 of CNTNAP2 gene were present in all samples.
Therefore, five samples (four from autistic patients and one from control sibling)
we
SiO2 nanostructure is synthesized by the Sol-Gel method and thin films are prepared using dip coating technique. The effect of laser densification is studied. X-ray Diffraction (XRD), Fourier Transformation Infrared Spectrometer (FTIR), and Field Emission Scanning Electron Microscopy (FESEM) are used to analyze the samples. The results show that the silica nanoparticles are successfully synthesized by the sol-gel method after laser densification. XRD patterns show that cristobalite structure is observed from diode laser (410 nm) rather than diode laser (532 nm). FESEM images showed that the shape of nano silica is spherical and the particles size is in nano range (? 100 nm). It is concluded that the spherical nanocrystal structure of silica
... Show MoreThe flavonoid rutin is the main content of the a queous extract of
the Iraqi plant Alhagi graecorum (family Leguminosae) which was
given to patients with different dental diseases and gave successful
results In this investigation , the amount of rutin is determined and was
found to be 4mg/100gm of the dried plant and it is equivalent to
4mg/500ml (0.8mg/100ml) of the mouthwash formula which was
prepared in this study This study reported for the first time the expiration date of rutin
in the prepared formula and was found to be 2.1 years. It also reported
for the first time that the ionic strength, buffer type and buffer concentration had no effect on the hydrolysis of rutin. Photochemical decomposition of
The acute toxicity effects of (2,4-D) pesticide to the common carp and grass carp was determined through 24, 48, 72 and 96 hr. Group of eight Common carp (Cyprinus carpio L., 1758; Pisces, Cyprinidae) and group of eight of grass carp (Ctenopharyngodon idella) were exposed to the 2,4-d. Lethal concentration (LC50) was determined with probit analysis. The median lethal concentration (LC50) values for common and grass carp were (157.77, 157.25, 156.41 and 152.62) and (115.92, 114.92, 112.94 and 96.52) respectively. Behavioral changes of the above mentioned species were examined for various herbicide concentrations.
The current study was conducted on 504(Ros-308) broiler chicks reared in Animal farms belong to College of Agriculture, University of Baghdad during the period 28/9/2017- 9/11/2018 to determine the effect of ginseng additive on the performance of chicks. Results of study showed a significant effect (p≤0.05) of exposure period an Red blood cells, 3.56×106ml3 of blood was in bird, which exposure to 2hr at heat shock. In 42 day at age 106 ×38 ml3 of blood can noticed in the blood at birds, which exposure to 2hr in 21-42 days at 3 days of age. No significant effect at ginseng on blood cells. The results showed a significant effect (p≤0.05) of interaction on red blood cells at 21 and 42 days of age and the average cells between these ages
... Show MoreBackground: Anti-oxidants are used as supplements to counteract the over production of free radicals in periodontal disease.Co-Q10 functions as an intercellular antioxidant by acting as a primary scavenger of free radicals (FRs) and reactive oxygen species (ROS), this study aimed to evaluate the effect of intra pocket application of perio Q gel (coenzyme Q10) alone and as adjunct to scaling and root planing on the periodontal clinical parameters in the treatment of patients with chronic periodontitis and compare the better improvement on the clinical periodontal parameters among different treatment modalities at 3 and 6 weeks. Materials and methods: A total of 323 sites with pocket depth (5-8) mm in patients with chronic periodontitis were
... Show MoreThe heavy metals mainly include Pb, Hg, Cd, Cr, Cu, Zn, Mn, Ni, Ag, etc. The heavy metals,Pb,Cu,Zn are considered most toxic to humans, fishes and environment. highly concentrations of heavy metals are harmful They destabilize ecosystems because of their bioaccumulation in organisms, and toxic effects on biota and even death in most living beings. All heavy metals, in spite some of them are essential micronutrients, have their toxic effects on living organisms via metabolic interference and mutagenesis. The bioaccumulation of toxic metals can happen in the body and food chain. So, the toxic metals generally exhibit chronic toxicity. The heavy metals like Pb has significant toxic effec