A simple, environmentally benign, sustainable, accurate and cost effective green approach has been developed for the determination of Acrylamide (2-propenamide) in different samples of potato chips collected from the Iraqi market during the year 2012 and a traditional Iraqi meal called Harissa. The method entails a straightforward de-fatting practice with n-hexane, extraction with lukewarm water, and cleanup with solid-phase extraction (SPE) cartridges containing the sorbent bed of the mixed mode ion exchangers (SiliaPrep C8/SCX-2/SAX). The final extracts are directly determined by liquid chromatography-Ultra violet (LC-UV) at a wavelength of 205nm for quantification. The acrylamide content in the examined potato chips were in the range 339–1024 μg/kg, while for Harissa sample it was 235 μg/kg. The recoveries were in the range of 97.4 and 101.2% with relative standard deviations (RSD%) of about 4.1%. The limit of detection (LOD) and the limit of quantification (LOQ) were 20 μg/kg and 50 μg/kg in the basis of signal to noise ratios of 3:1 and 9:1, respectively. The green goal of the proposed method was accomplished by developing a procedure that uses minimal amounts of benign reagents and the avoidance of producing toxic residues. This method can be used for routine analysis of Acrylamide in any fatty food matrices.
The role of transmembrane protease serine 2(TMPRSS2) in prostate carcinogenesis relies on overexpression of ETS transcription factors. The aim of this article was to investigate the association of TMPRSS2 polymorphism (rs12329760 (C\T)) with prostate cancer (PCa) in sample of Iraqi patients. One hundred and two individuals were involved in this study for the period from February – 2019 to February – 2020. The sample type was formalin fixed paraffin embedded tissue samples (FFPE), which involved fifty-six samples of pre-diagnosed patients with prostate cancer, aged between 48 and 86 years, and forty-six samples were found to be controls (healthy group) dependent on Prostate Gland integrity, which is the same age as in a group o
... Show MoreThe current study was designed to compare some of the vital markers in the sera of diabetic and neuropathy patients via estimating Adipsin, Fasting blood Glucose(FBG), Glycated(HbA1c) hemoglobin, Homeostasis Model Assessment Index (Homa IR ), Cholesterol, High density lipoprotein (HDL), Triglycerides (T.G), Low-density, and lipoprotein (LDL), Very Low Density Lipoprotein (VLDL), in sera of Iraqi patients with diabetes and neuropathy. A total of ninety subjects were divided into three groups: group I (30 diabetic with neuropathy males) and group II (30 diabetic males without neuropathy), and 30 healthy sujects were employed as control group. The results showed a significant decline in Adipsin levels (p>0.05) in neuropathy, T2DM g
... Show MoreBackground: Human leukocyte antigen (HLA) is the most polymorphic genetic system in man. The genes of this region seem to influence susceptibility to certain diseases.
Patients and methods: Polymerase chain reaction-Sequence Specific Primers PCR-SSP is the method used to asses HLA-typing of 100 blood samples of 60 AIH patients and 40 healthy normal controls.
Results: An increased frequency of HLA-DR3, DR4 and DR7 was observed for patients group versus control group with P-value (0.0001, 0.05, and 0.001) respectively, while DR*0211 (DR2) may be formed the basis for protection against the disease. HLA-DQ on the other hand, yielded on association in Iraqi patients with AIH.
Conclusions: This finding de
The aim of the present research is to study different protein fractions in sera of children and adolescent with β –thalassemia major and minor and to compare the results with that of healthy control.One hundred fifty children and adolescents were enrolled in this study,including 50 patients with β- thalassemia major , 50 patients with β- thalassemia minor as pathological control group and another apparently 50 healthy individuals as a control group. The age of all studied groups ranged from (4-18)years.Total protein, albumin and immunoglobulins were estimated in sera of all subjects. A Significant decrease was found in the total protein and albumin&nb
... Show MoreEndocrine Abnormalities in β-thalassemia major are common disturbing
complications, that need prompt management. Importance of this work was to
evaluate the some biochemical parameters and endocrine hormones related to
the pubertal maturity and fertility status in cases with thalassemia. A sixty patients
[38 males and 22 females] with β - thalassemia major against 30 healthy subjects
[17 males and 13 females] were enrolle . The Blood levels of, Leptin, Vitamin D,
thyroid function test, parathyroid hormone, ghrelin, and sex hormones were
determined in the blood. Body Mass Index (BMI) was also evaluate. Results
showed that besides lower BMI, all hormones assessed were significantly lower in
thalassemia β-major
The researcher seeks to know the extent of applying the concept of design by Iraqi electronic press sites to provide media product with the acceptance of their browsers and ensure their repeated visits to such sites in the framework of the guiding rules for laser browsing. The researcher uses analytical study on a sample of six Iraqi electronic newspaper sites to identify the general features of their design and the methods of distribution of the structural elements of the pages. The researcher also conducts a field study on a sample of the surfers of electronic newspaper sites to see the extent of their evaluation to their design and the degree of their satisfaction.
There are numerous bidirectional interactions between the reproductive system and the liver. Sex steroids regulate metabolic health through signaling effects in both peripheral and central metabolic tissues, including adipose tissue, liver, skeletal muscle, and brain, and have a role in the etiology of structural and functional liver diseases. Blood samples were obtained from 90 healthy women (control group) and 90 women that have hormonal changes (patients’ group). The levels of reproductive hormones (follicle stimulation hormone/FSH, luteinizing hormone/LH, estradiol/E2, progesterone/P4) were measured by using fully automated Cobas E411, whereas those of liver enzymes (alanine transaminase /ALT, aspartate aminotransferase/AST, a
... Show MoreBackground: Human leukocyte antigen (HLA) is the most polymorphic genetic system in main.
The objective of this study was to investigate the morphological description and histological structure of adrenal gland in Iraqi black partridge Francolinus francolinus from Iraqi environment. Twenty adult healthy birds (male and female) were used in present study. They were measured and adrenal glands were removed, measured and fixed by 10% formalin and formalin and chromate fixative then stained with rotein histochemical stain (Harris hematoxylin and eosin stain (H&E), periodic acid Schiff (PAS), Azan and Mallory stain). The partridge had a small couple of adrenal glands of yellow colour they are located in abdominal cavity nearby the margin of superior kidney lobe. These are small, ovoid glands lying just cranial to the kidneys a
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