The objective of this study was to investigate and find the relationships between human semen osmolality and oxidative stress with semen quality. Semen analysis was determined to evaluate semen quality and fertility. The concentration of semen malondialdehyde (MDA) was measured to assess oxidative stress. For this purpose, one hundred seventy healthy adult males were used in this study. The study was conducted from September 2018 to November 2019 in the Infertility care and in vitro fertilization center (IVF) and the Biology department of the College of Science at Salahaddin University in Erbil city. A significant positive correlation was observed between the total sperm motility, grade activity, and sperm motility index with semen osmolality less than 300 mOsm kg-1 (r= 0.62, r = 0.64, and r= 0.75 p≤0.01 respectively) and osmolality 300-350 (r= 0.53, p≤0.05 r= 0.52 p≤0.05, and r= 0.56 p≤0.01 respectively). Total sperm motility, grade activity, and sperm motility index are negatively correlated with osmolality 351-400 mOsm kg-1 (r= -0.65 p≤0.05, r= -0.56 p≤0.05, and r= -0.67 p≤0.01) and more than 400 mOsm kg-1 (r= -0.86, r = -0.74 and r= -0.88 p≤0.01). Regarding the relation between oxidative stress and sperm motility kinetics, total sperm motility, grade activity, and sperm motility index is negatively correlated with MDA more than 2 µmol/L (r= -0.56 p≤0.05, r= -0.52 p≤0.05, and r= -0.67 p≤0.01 respectively). No significant correlation was found between semen osmolality and MDA concentration with sperm concentration, total sperm count, sperm viability, and normal sperm morphology.
This study was designed to investigate the effects of licorice extract (Glycyrrhiza glabra L.) addition to semen diluters on ram sperm progressive motility during storage at 5 ˚C for 72 h. Semen was collected from 3 proven Awassi rams. Licorice extract powder was added at levels of 1, 5, 10, 50 and 100 µg per ml. of diluter. Diluter containing no licorice extracts served as control (0).Progressive motility was estimated subjectively after dilution (0h), and at 24, 48, and 72 h of storage. The experiment was replicated 2 times with egg yolk-tris (EYT) diluter and 2 times with yolk- glucose-citrate diluter. Progressive motility increased significantly (p < 0.01) in levels of licorice extract 1, 5, 10, 50 and 100 µg / ml
... Show MoreThis study was carried out at the Tissue Culture Laboratory, which belongs to the General Commission of Biotechnology, in order to evaluate the influence of NaCl-induced salinity stress on some oxidative parameters (MDA and H2O2) and antioxidant enzymes (CAT, APX, and SOD) in eight durum wheat genotypes (Doma1, Bouhoth11, Cham3, Bezater, Cham5, Aghamatales, Icaverve, and Icamber) in vitro, to determine the most salinity tolerant genotypes. Salinity stress was applied by adding different levels of NaCl to the growing medium (0, 50, 100, and 150 mM). The experiment was laid according to a complete randomized design (CRD) with sixteen replicates. Increasing
... Show MoreThe possible effects of COVID-19 vaccines on reproductive health and male fertility in particular have been discussed intensely by the scientific community and the public since their introduction during the pandemic. On news outlets and social media platforms, many claims have been raised regarding the deleterious effects of COVID-19 vaccines on sperm quality without scientific evidence. In response to this emerging conflict, we designed this study to evaluate and assess the effect of the Pfizer-BioNTech mRNA COVID-19 vaccine on male fertility represented by the semen analysis parameters.
The aim of this study is to find a relationship between oxidative stress and adiponectin in Iraqi patients with acromegaly. The present study included 30 patients with acromegaly disease attending at Al-Yarmuk teaching hospital , and 30 healthy individuals as a control group.The two groups with ages ranging (30-55) years. The results revealed a highly significant elevation in all parameters (GH,IGF-1 , adiponectin , malondialdehyde , and peroxynitrite ) levels in sera of patients when compared with healthy control .It can be concluded that oxidative stress (malondialdehyde and peroxynitrite ) may be valuable in detecting of endocrine diseases like acromegaly .
Back ground:-Rheumatoid arthritis (RA) is a systemic disease characterized by progressive, erosive and chronic poly arthritis, where cellular proliferation of the synoviocytes and neo-angiogenesis leads to formation of pannus which destroys the articular cartilage and the bone .
Objective :-to evaluate theOxidative Stress index( OSI )by measuring malondialdehide( MDA) and Total Antioxidant Capacity( TAC) in serum of RA patients in addition to uric acid and albumin and to investigate the alteration in these parameters compared to healthy individuals.
Patients and Methods :- twenty five patients with rheumatoid arithritis and twenty five apparen
... Show MoreThis Study was conducted out at the Ministry of Agriculture's Poultry Research Station/Animal Resources Department/Agricultural Research Center. To see how body weight (BW) and leptin hormone (LEP) levels in breeder blood affect fertility and hatchability. 140 Iraqi local laying chickens (120 females + 20 males) aged 28 weeks were used in the study. Following the numbering of females, the birds were grown in individual cages and dispersed sequentially on cages. The experiment was divided into three periods, each lasting 28 days, during which the breeder's live body weight was recorded and divided into two categories (greater than 1.5 kg and less than 1.5 kg), and blood samples were collected at the end of each period to determine th
... Show MoreThe current research deals with spatial relations as a tool to link urban landmarks in a homogeneous composition with monumental sculptures, by identifying these landmarks and the extent of their impact on them, which constitutes an urgent need to evaluate the appropriate place and its effects on them, so that this analytical study is a critical approach adopted in artistic studies of monumental models in Arabcapitals .The current research came in four chapters, the first chapter of which dealt with the research problem, its importance and the need for it, then its objectives that were determined in revealing the spatial relations and their impact on
... Show MoreBackground: In the last years, Endometriosis affects up to 10 percent of reproductive aged Iraqi women. Little is known about the etiology of endometriosis in Iraqi women.
Objectives: The aim of this study is to investigate the hormonal changes, antioxidant status, Coenzyme Q10 and oxidative stress in women patients with endometriosis.
Patients and methods: 30 endometriotic women mean aged (31.16±5.61 year ) who were undergoing laparoscopy to participate in this study, and (n=30) healthy women volunteers mean aged (31.96±5.42 year) as control group.
Results: The results in this study revealed a highly significant increased (P<0.01) in estradiol (E2), uric acid (UA) and oxidative stress (MDA). The results of the Coenzyme Q10
Background:
type 2 diabetes mellitus is a chronic disease that is accompanied by the production of free radicals which will ultimately increase the level of oxidative stress.
The present study was designed to find the relation between oxidative stress and
pregnancy. The study used one hundred twenty volunteers (ninety pregnant women
and thirty non-pregnant women). Then pregnant women were divided to three
subgroups according to trimester (first trimester, second trimester and third
trimester). then, some oxidative stress factors (MDA, GSH, catalase and TAC) were
measurement. Biochemical tests showed significant (P<0.05) increase in
malonedialdehyied (MDA) and significant (P<0.05) decrease in Glutathione (GSH),
Catalase (CAT) and total antioxidant capacity (TAC) in pregnant women compared
with non-pregnant women group. It was concluded from this study that the
pregnancy led to