From different hospitals in Baghdad city, 25 clinical isolates of Proteus spp. were collected from different clinical samples, all isolates were identified as Proteus mirabilis by using bacteriological and biochemical assays in addition to Vitek-2 identification system. 15 (60%) isolates were identifying as Proteus mirabilis. The susceptibility of P. mirabilis isolates towards cefotaxime and ceftazidime was (66.6 %), (20%) consecutively; while extended spectrum β-lactamases producing P. mirabilis percentage was (30.7 %). Because blaVEB-1 was documented as an important indicator for increasing risk of extended spectrum beta ßlactamases producing P. mirabilis isolates that began to spread from many geographic area to Far east which inc

A significant increase in the incidence of non-O157 verotoxigenic Escherichia coli (VTEC) infections have become a serious health issues, and this situation is worsening due to the dissemination of plasmid mediated multidrug-resistant microorganisms worldwide. This study aims to investigate the presence of plasmid-mediated verotoxin gene in non-O157 E. coli. Standard microbiological techniques identified a total of 137 E. coli isolates. The plasmid was detected by Perfectprep Plasmid Mini preparation kit. These isolates were subjected to disk diffusion assay, and plasmid curing with ethidium bromide treatment. The plasmid containing isolates were subjected to a polymerase chain reaction (PCR) for investigating

Several toxigenic cyanobacteria produce the cyanotoxin (microcystin). Being a health and environmental hazard, screening of water sources for the presence of microcystin is increasingly becoming a recommended environmental procedure in many countries of the world. This study was conducted to assess the ability of freshwater cyanobacterial species Westiellopsis prolifica to produce microcystins in Iraqi freshwaters via using molecular and immunological tools. The toxigenicity of W. prolifica was compared via laboratory experiments with other dominant bloom-forming cyanobacteria isolated from the Tigris River: Microcystis aeruginosa, Chroococcus turigidus, Nostoc carneum, and Lyngbya sp. signifi

Two quantitative, environment-friendly and easily monitored assays for Ni (II) and Co (III) ions analysis in different lipstick samples collected from 500-Iraqi dinars stores located in Baghdad were introduced. The study was based on the reaction of nickel (II) ions with dimethylglyoxime (DMG) reagent and the reaction of cobalt (III) ions with 1-nitroso-2-naphthol (NN) reagent to produce colored products. The color change was measured by spectrophotometric method at 565 nm and 430 nm for Ni and Co, respectively, with linear calibration graphs in the concentration range 0.25-100 mg L^-1 (Ni) and 0.5-100 mg L^-1 (Co) and LOD and LOQ of 0.11 mg L^-1 and 0.36 mg L^-1 (Ni), and 0.15 mg L^-1 an

The objective of this study was to establish an accurate, precise, sensitive, simple, fast and reliable method for the determination of ciprofloxacin hydrochloride in pure or in pharmaceutical dosage forms using Homemade instrument fluorimeter continuous flow injection analyser with solid state laser (405 nm) as a source. The method is based upon the fluorescence fluorescein sodium salt and its quenching by ciprofloxacin hydrochloride in aqueous medium. The solutions of standard and the sample were prepared in distilled water. The calibration graph was linear in the concentration range of using (10 - 100) mMol.L-1 ciprofloxacin hydrochloride (r= 0.9891) with relative standard deviation (RSD%) for 3 mMol.L-1 ciprofloxacin HCl solution is

In this paper, the rotation curve of the Milky Way galaxy has been determined using the observed HI emission line at a wavelength of 21 cm. Particularly, the Tangent Point Method was used in order to measure the rotational velocity and the distance to the center of the Milky Way. The measured rotation curve showed that the rotational velocity remains approximately constant at large distances from the center of the Galaxy. This is actually an evidence for the existence of dark matter in the halo of the Milky Way. If all the matter in the Milky Way is visible, then the behavior of the rotation curve of the galaxy should experience a Keplerian decline. The mass of the Milky Way within a radius of 15 kpc was also estimated to be ~ 1.65 × 10