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jih-1847
Detection and Detoxification of Aflatoxin B1 from Fish Feedstuff Using Microwave and Ozone Gas
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    The current study was designed to investigate the occurrence of aflatoxin B1 in thirty two samples of fish feedstuff were collected randomly from some Iraqi local markets using ELISA technique. Aflatoxin B1 was detected in thirty samples and the concentration of toxin ranged from 50 ppb to 1000 ppb.  

   Microwave and ozone were used for detoxification of aflatoxin B1 from sample with highest concentration (1000 ppb), two degree of temperature and two times (50°C and 100°C for 5 minute and 10 minute to each degree) of microwave, also two doses and two times (2 g and 4 g for 5 minute and 10 minute to each dose) of ozone gas were used.

   Degradation of aflatoxin B1 by microwave has been found to cause a significant (P ≤ 0.05) decrease of aflatoxin B1, Moreover, the concentration of aflatoxin B1 was dependent on temperature degrees and exposure time, also sample subjected to ozone gas caused a significant (P ≤ 0.05) decrease in aflatoxin B1 contents and the concentration of aflatoxin B1 was dependent on doses and times of exposure. Results showed that ozone gas was more effective in aflatoxin B1 reduction when compared with microwave.

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Publication Date
Mon Oct 01 2012
Journal Name
International Journal Of Recent Scientific Research
DETECTION OF LTH, FSH AND LH HORMONE LEVEL IN PREGNANT WOMEN INFECTED WITH TOXOPLASMA GONDII
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Infection with the protozoan parasite Toxoplasma gondii is widely prevalent in humans and animals. Infection with Toxoplasma may associate with miscarriage in many pregnant women due to infection. In this study, the level of lutetropic hormone (LTH), folliclestimulating hormone (FSH) and luteinizing hormone (LH) was measured in pregnant women suffering from toxoplasmosis using mini-VIDAS®technique. Results showed that pregnant women have high concentration of both LTH and FSH hormone(10.80 ± 6.53) ng/ml and (9.51 ± 2.40) μIU/ml respectively, while the concentration of LH hormone was lower than normal(4.49 ± 0.56) μIU/ml. Such finding is to suggest that infection with T. gondii is interfering with these hormones in pregnant women.

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Publication Date
Thu Jun 29 2023
Journal Name
Journal Of University Of Babylon For Pure And Applied Sciences
Genetic Detection of IMP-1 Gene and its Relationship with Biofilm Formation in Klebsiella pneumonia
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Background: Klebsiella pneumoniae were considered as normal flora of skin, and intestine. It can cause damage to human lungs; the danger of this bacterium is related to exposure to the hospital surroundings. materials and methods: the detection of Klebsiella pneumoniae on morphological and biochemical tests and then assured with VITEK 2 system. Resistance to antibiotics was determined by Kirby-Baeur method. And genotyping of IMP-1 in isolates was done by PCR technique, then biofilm formation was identified by Micro titer plate method. Results: The present study included a collecting of 50 specimens from different clinical specimens, (blood 40%, urine 30%, sputum 20%, wound infection 10%); 10 isolates were identified as K

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Publication Date
Thu Apr 18 2019
Journal Name
Al-kindy College Medical Journal
Basal cell markers:34BE12 and p63, improving detection of basal cells in atypical prostatic lesions
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Background: The diagnosis of prostatic pathology may be of challenging , as some  difficult and suspected, atypical  cases may lack basal cell layer by routine H&E sections . Antibodies against 34BE12(HMW-CK) and p63 aid the diagnosis of such cases , to distinguish benign from  malignant prostatic lesions.

Objective: to identify basal cells in atypical prostatic lesions ,and distinguish benign from malignant prostatic lesions.

Type of the study:  A retro-spective  study.

Methods:  115cases of  paraffin embedded prostatic tissue blocks ,diagnosed as : 76 cases were benign prostatic hy

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Publication Date
Wed Feb 22 2023
Journal Name
Iraqi Journal Of Science
Rapid Direct Detection and Differentiation of Mycobacterium tuberculosis complex in Sputum by Real-Time PCR
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Tuberculosis status as the second leading causes of significant morbidity and mortality from an infectious disease worldwide, after human immunodeficiency virus (HIV). Sample collection was conducted at the Institute of Chest and Respiratory Diseases/Baghdad Medical City in Baghdad. The collection interval was from August to October 2014, 629 suspected TB patients were examined during this period. The results revealed among total 629 specimens, 56 (8.9%) of the specimens were positive by direct examination and 573 (91.1%) negative specimens by smear microscopy. Fifty six DNA samples were extracted from positive ZN smears of sputum specimens and 40 samples from healthy persons (as control) were subjected to molecular diagnosis by real tim

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Publication Date
Wed May 04 2022
Journal Name
Int. J. Nonlinear Anal. Appl.
Knee Meniscus Segmentation and Tear Detection Based On Magnitic Resonacis Images: A Review of Literature
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The meniscus has a crucial function in human anatomy, and Magnetic Resonance Imaging (M.R.I.) plays an essential role in meniscus assessment. It is difficult to identify cartilage lesions using typical image processing approaches because the M.R.I. data is so diverse. An M.R.I. data sequence comprises numerous images, and the attributes area we are searching for may differ from each image in the series. Therefore, feature extraction gets more complicated, hence specifically, traditional image processing becomes very complex. In traditional image processing, a human tells a computer what should be there, but a deep learning (D.L.) algorithm extracts the features of what is already there automatically. The surface changes become valuable when

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Publication Date
Sun Dec 01 2013
Journal Name
Toxicon
Methods for simultaneous detection of the cyanotoxins BMAA, DABA, and anatoxin-a in environmental samples
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Publication Date
Sat Dec 18 2021
Journal Name
Iraqi Journal Of Science
Detection The Prevalence of Adhesins and Extracellular hydrolytic enzymes genes in Candida albicans Biofilm Formation
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Biofilm formation (BF) is one of the most important virulence factors of
Candida spp. The aim of this study was to detect the prevalence of genes
responsible in biofilm formation of C. albicans by conventional PCR technique.
Among 49 vaginal specimens (VC), C. albicans was the most predominant species
in percentage 22/49 (45%) and 27(55%) were non albicans. Out of 47 oral
specimens (OS), 22/47(47%) were C. albicans, whereas 25(53%) were non albicans.
At the present study; all C. albicans were biofilm producers with variable strength,
out of 44 BF producers, 18 (40.9%) were low biofilm (LBF) with significant
differences (P<0.05) between HVS and OS, 25 (56.8%) moderate or high biofilm
(HBF) and just one isolat

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Publication Date
Sun Jan 01 2023
Journal Name
Inorganic Chemistry Communications
Detection of nitrotyrosine (Alzheimer's agent) by B24N24 nano cluster: A comparative DFT and QTAIM insight
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A nano-sensor for nitrotyrosine (NT) molecule was found by studying the interactions of NT molecule with new B24N24 nanocages. It was calculated using density functionals in this case. The predicted adsorption mechanisms included physical and chemical adsorption with the adsorption energy of −2.76 to −4.60 and −11.28 to −15.65 kcal mol−1, respectively. The findings show that an NT molecule greatly increases the electrical conductivity of a nanocage by creating electronic noise. Moreover, NT adsorption in the most stable complexes significantly affects the Fermi level and the work function. This means the B24N24 nanocage can detect NT as a Φ–type sensor. The recovery time was determined to be 0.3 s. The sensitivity of pure BN na

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Publication Date
Fri Feb 26 2021
Journal Name
Iraqi Journal Of Science
Detection of Pro-Inflammatory IL-8 and IL-12 in Iraqi Women Infected with Trichomoniasis
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Trichomonas vaginalis is a unicellular flagellated protozoan that resides in female and male genital tract and considered the most prevalent sexually transmitted infectious parasite. The infection rate is relatively equivalent between male and female but trichomoniasis is usually asymptomatic in men. Primary triggering of host inflammatory response to this parasite is not fully understood and most studies address the local reaction of the parasite in female genital tract. In this study, two pro-inflammatory cytokines, IL-8 and IL-12, were investigated in the serum of infected women with Trichomonas vaginalis during acute and chronic stages of the disease. The results demonstrated that the level of IL-8 was significantly

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Publication Date
Mon Apr 01 2024
Journal Name
South African Journal Of Chemical Engineering
Removal of COD from petroleum refinery wastewater by adsorption using activated carbon derived from avocado plant
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