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jih-1774
Antimicrobial activity of some plants extracts on bacteria isolated from acne vulgaris patients
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Background: Acne is a cutaneous pleomorphic disorder skin disease most frequently occurring during the adolescent in ages of 12-24, with estimated  percentage ( 85%) . There are different ways to treat acne such as  using of antibiotics  , herpes , and mixing treatments .

Methods : Antibacterial activity  of  four concentrations (100,50,25,12.5)mg /ml of  alcoholic  and cold  aqueous  crude extracts of Cinnamon(Cinnamomum verum ), Henna (Lawsonia inermis ) , Lupine (Lupinus luteus) were studied against aerobic and  an aerobic bacteria isolated from inflamed and discharging pus  from thirty Iraqi acne vulgaris patients refer to dermatology unit  at AL-Kindy Hospital from December 2016  to March 2017.All information (age, sex ,diseases and using topical treatments) were recorded .The bacterial isolates  were identify using morphological characteristics, biochemical tests and  the  Vitek-2 compact system.

Results: Among (30 )Acne samples taken, 8 (26.7%) samples were from males age range (19-33) years and 22(73.3%) were from females within age (17-29)years . twenty five (83%) samples  were culture positive, and only  (17%) of samples revealed no growth .Most frequent  bacteria which isolated  (aerobically) from acne patients were  Staphylococcus aureus ( 60%), Staphylococcus epidermidis (20%),  Escherichia coli  (8% ),  Pseudomonas aeruginosa  (4%), and an aerobic bacterial isolates  were Propionibacterium acnes  (8%) isolates.

 

Antibiotic sensitivity tests were performed against, Ampicillin, Clindamycin, Gentamicin, Cotrimoxazole, Erythromycin, Vancomicine, Tetracycline, Doxycycline, and Azithromycin.All bacterial isolates were resistance to Ampicillin. Staphylococcus aureus and S. epidermidis were sensitive (100%) to Doxycycline and Azithromycin, P. acne were also highly sensitive to these two antibiotics (95.5%,97.1%) respectively, while E. coli and P. aeruginosa were (100%) resistance to these antibiotics. Gentamicin and tetracycline were susceptible by most of the study isolates except for P. aeruginosa which was very resistance to CN and TE (100%&94.8%) respectively.

The antimicrobial potential of cold water and alcoholic crude extracts of Cinnamon (Cinnamomum verum ), Hinna (Lawsonia inermis), and Lupine (Lupinus luteus), in concentrations (100,50,25,12.5) mg/ml against the gram positive and gram negative isolates were tested through a well-diffusion method.

Alcoholic extract of  Henna leaves in concentration  (100mg/ml) showed high inhibitory activity  to all isolates compared with the aqueous extract and the all concentration of cinnamon aqueous and alcoholic extracts ,while  lupine extracts  had no effect on all  bacterial isolates   .

Conclusion: Gram-positive bacteria were the most common microorganisms involved  in  acne infection. There are variations in the incidence of acne infection in relation to sex, age; Alcoholic extract of the Henna leaves could be used to treat acne.

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Publication Date
Fri Dec 01 2023
Journal Name
Baghdad Science Journal
Antioxidant Activity of Endophytic Bacteria isolated from (Pyrrosia piloselloides) (L) M.G. Price
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Endophytic bacteria produced analogous secondary metabolites of their hosts. Similarly, the ability to generate antioxidants is not an exception. Dragon scales (Pyrrosia piloselloides), an epiphytic plant of the Polypodiaceae family, are frequently overlooked. This research aims to isolate antioxidant-producing bacteria from dragon-scale fern leaves. The antioxidant activities were tested after the extraction procedure using ethanolic extract. Bacteria were characterized and selected as candidates for antioxidant production by screening for the production of total phenolic compounds. Antioxidant levels were determined utilizing the ABTS, FRAP, and DPPH techniques. The preliminary findings of the entire phenolic compound test rev

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Publication Date
Thu Jun 30 2016
Journal Name
Al-kindy College Medical Journal
Prevalence of bacteria isolated from patients with burn infection at a burn hospital in Baghdad and study of their antimicrobial susceptibility patterns
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Background: The skin functions as a barrier to the external environment, damage to this barrier following a burn disrupts the innate immune system and increases susceptibility to bacterial infection. Objective: This study was carried out to determine the bacterial isolates and study their antimicrobial susceptibility in burned wound infections at one burn's hospital in Baghdad.Type of study:Cross-sectional study.Methods: The bacteria were identified at species level by using Analytic Profile Index (API) system and The antimicrobial susceptibility test was performed according to Kirby-Bauer (disk diffusion) technique.Results: Over a period of one year (from October 2014 to October 2015). Out of 848 patients with different degrees of burns

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Publication Date
Wed Jun 30 2021
Journal Name
Annals Of R.s.c.b.
Multidrug Resistant Enteric Bacteria Isolated From Patients With Urinary Tract Infections
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Background and Objectives: Urinary tract infections (UTIs), among a wide range of microbial infections, are of a double-edged worry with health-care and economic implications. They are serious diseases that can influence various parts of the urinary tract. The aim of this study was characterization of the enteric bacteria isolated from urine of human UTIs and studying their antimicrobial sensitivity. Materials and methods: A total of 50 urine samples were collected from patients with UTIs of both genders. The isolates identification was done using routine diagnostic methods and confirmed by Vitek2. Antimicrobial susceptibility was done against 10 antimicrobials. Results: Both genders of human were found to suffer from urinary tract problems

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Publication Date
Sun Apr 15 2001
Journal Name
University Of Baghdad, College Of Veterinary Medicine
A Study of Antimicrobial Effect of Oil Extracts of Black Seed (Nigella sativa L.) on some Pathogenic Microorganism that is isolated from Clinical Pathogenic Cases.
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Publication Date
Mon Apr 01 2013
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Azithromycin Versus Co-trimoxazole in The Treatment of Doxycycline Resistant Acne Vulgaris In Iraqi Patients
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Background: Acne is a multifactorial disease with multiple pathogenic factors. Many types of therapies are available including systemic antibiotics , choosing the appropriate one depend on the experience of the doctor , side effects , antimicrobial resistance , availability and cost. Although doxycycline is effective and commonly prescribed as first line systemic treatment for moderate to severe inflammatory acne vulgaris , some cases do not respond because of antimicrobial resistance.

Objective: To evaluate and compare the effectiveness and safety of azithromycin and co-trimoxazole in the treatment of acne vulgaris cases that are resistant to doxycycline  in Iraqi patients.

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Publication Date
Wed Jan 30 2013
Journal Name
Al-kindy College Medical Journal
The efficacy and tolerability of 10mg and 20mg/day isotretinoinin the treatment of acne vulgaris in Iraqi patients
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Back ground: Oral isotretinoin is recommended
for sever nodulocystic acne in the doses 0.5-
2mg/kg/day which is usually associated with higher
incidence of adverse effects. To reduce the
incidence of side-effects and to make it more costeffective,
the lower dose regimen of isotretinoin has
been used.
Aim: To compare the efficacy and tolerability of
oral isotretinoin 10mg and 20mg/day in acne
vulgaris.
Methods: one hundred and twenty patients with
acne vulgaris were randomized into two treatment
regimens each consisting of 60 patients. The first
was treated with 10mg/day and the second group
with 20mg/day for 24 weeks. Fifty five patients
from the first group and 47 patients from the second

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Publication Date
Sun Jun 01 2014
Journal Name
Baghdad Science Journal
Effect of Citrus aurantifolia Seed Extracts In Some Bacteria Isolated From Burns Infections: Suaad K. Ibrahim|Ilham S. Banno|Sawsan M. Abdella
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The present study was carried to evaluate antibacterial activity of Acetone, Alcholic (cold and hot) and Aqueaus(water) extracts of Citrus aurantifoliaseeds,against growth of some bacteria isolated from burns infections(Pseudomonas aeruginosa,Escherichia coli, Klebsiellapneumonia,Staphylococcusaureus)fromKindy HospitalIn Baghdad from March to June 2012.Antibiotic Sensitivity was done for all isolated bacteria used in this study.Results showed variation in antibacterial activity of different extracts against all tested bacteria by well diffusion technique in agar and measuring the diameter of inhibition zone, at concentration 250Mg-ml. Acetone extract had the greatest inhibitory effect followed by hot alcoholci extract, and then cold alcohol

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Publication Date
Sun Mar 01 2009
Journal Name
Baghdad Science Journal
Effect of leaves Extracts of Duranta repens on growth and activity of some types of Pathogenic Bacteria and Some types of Fungi
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A study were conducted to examinate the effect of organic and aqueous (Hot, Cold) Extracts from leaves of Duranta repens on the growth and activities of the following types of Bacteria:- Staphylococcus aureus,Streptococcus pyogens ,Escherichia coli,Klebsilla pneumonia, in addition to the yeast Candida albicans and the fungi Aspergullis niger ,Aspergulls flavus.The result showed that gram Positive Bacteria is more sensitive to the extracts than gram negative bacteria with Minimum inhibitory concentration (MIC) value (50,25,50,100)% and Minimum Bactericidal Concentration (MBC) value (100,50,200,100)% for all types Bacteria respectively . The most active extract against A.niger ,A,flavus was cold and hot aqueous extract from the leaves with d

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Crossref
Publication Date
Tue Sep 25 2018
Journal Name
Iraqi Journal Of Science
Estimate Antimicrobial activity and Anti-biofilm formation of bark Cinnamomum zeylanicum on Klebsiella pneumoniae isolated from Urinary Tract Infections
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Five isolates (25%) of Klebsiella pneumoniae were isolated from urine samples. In addition also isolated bacteria were (10) 50% Escherichia coli, while (3)15% Proteus spp., (2)10% Pseudomonas aeruginosa. The ethanolic extract of Cinnamomum zeylanicum bark were tested against Klebsiella pneumoniae by using the well agar diffusion test, the alcoholic bark extract from (200 -12.5) mg/ml possessed antimicrobial activity against tested microorganism. At 200 mg/ml, and 100 mg/ml concentrations was diameter of inhibition zone rang from (18-26mm), (14-16mm) respectively, and these results compared to antibiotics Norfloxacin(10µg) inhibition zone (24-30mm), and Cefotaxim (10 µg) (26-27mm) as

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Publication Date
Mon Apr 10 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Evaluation of Antibacterial Activity of Ethanolic Extracts for Three Local Plants
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There is an increasing interest in the use of plant extracts as therapeutic agents, particularly their capacity to inhibit the growth of pathogenic microorganisms. In this study antibacterial effect of Malva sylvestris, Anastatica hierochuntica and Vitis vinifera leaves extracts were evaluated against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus and Proteus mirabilis. The in vitro antibacterial activity was performed using agar well diffusion method and the minimum inhibitory concentration (MIC) was determined by microtitration technique. The result indicated that the extract of V. vinifera leaves inhibited with the growth of gram-positive bacteria, as well as gram-negative bacteria while the extract

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