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The present study was undertaken in order to investigate the role of gentamicin in the gene expression of toxA
in Pseudomonas aeruginosa isolated from cow mastitis. A total of ten P. aeruginosa strains originally isolated from cows
infected with mastitis. Agar dilution methodology was performed to determine the minimal inhibitory concentration
of gentamicin, all of which developed resistance toward gentamicin. The findings presented here demonstrated that
all these strains harboured toxA depending on PCR-based assay. Nonetheless, RT-PCR technique revealed a wide
variation in expression of toxA. Moreover, the cultivation of P. aeruginosa in the presence of gentamicin, significantly
(P< 0.05), induced the expression of toxA, in addition to th
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