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Five isolates of Gram negative bacteria (Klebsiella pneumoniae, Psuedomonas auroginosa, proteus
mirabilis and two strains of E.coli) were in quested for the ability of bearing silver nanoparticles by using
LB medium, all the isolates of bacteria were buttered brown color just as soon as mixed the supernatant
of bacterial culture with AgNO3 solution, that refered the biosynthesis of Silver nanoparticles (Ag NPs).
UV–visible spectrophotometer and Fourier transform infrared (FTIR) spectroscopy were utilized for
estimation of (Ag NPs). The five isolates of bacteria were tendered to produce spontaneous mutants by
using different kinds of antibiotics, Ampicillin put to use for making mutant in E.coli and Proteus
mirabillis, while Pseudom
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