Acid phosphatase
obese-diabetes mellitus patients
purification of acid phosphatase
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The study aimed to purification of acid phosphatase (ACP) from sera of obesetype 2 diabetes mellitus patients,
this study included from thirty T2DM patients and thirty control, purification process was done with several steps included
precipitation with inorganic salt (NH4
) 2SO4
30%-80%, dialysis, ion exchange chromatography by DEAE sepharose anion
column and size exclusion chromatography by Sepharose 6B.ACP, BMI, FBS, HbA1c, Lipid profile, Urea, Creatinie, Insuline,
Homa-IR were determined. Results showed the precipitate and concentrated protein appeared four peaks in ion exchange
column. ACP located in the first and second peak with purification fold (21.1), (37.2) yield of enzyme and specific activity (173.3)
IU/ml, which obtained a si
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