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Seeds of the two rice genotypes namely Amber 33 (A33) and Amber Baghdad
(AB) were divided into two groups; the first was presoaked in different
concentrations of ethyl methane sulphonate (EMS) as chemical mutagen for
different duration times (3, 6 and 12) hrs, and the other was exposed to different
exposure times of ultra violate (UV-B) radiation (280-320 nm) as physical mutagen
for different times (20, 40 and 60) min at room temperature. Treated and non-treated
seeds were transferred into the callus induction medium containing 2.5 mg/L 2,4-
dichlorophenoxy acetic acid (2,4-D) and 0.5 mg/L benzyl adenine (BA) under
aseptic conditions. Calli were divided into two groups the first was treated with
several EMS concentrations (0.0, 0.50. 1.0, 1
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