Fifty isolates of Psel.ldomonas aeruginosa were obtained from
(170) isoiates of ctlinical cases. Sensitivity of the isolates t() antibiotic leveled showed a high resistance to cefotaxime, ceftazidime, gentamicin and tobramycin. To less extent was the resistance to· amikacin and ciprofloxacine. All isolates of Pseudomonas aeru,ginosa were highly sensitive tocefepime and imipenem.
Eighty six percent oJ the isolates of Pseudomonas aeruginosa produced protease enzyme .The isolatt;: Number (2) of Pseudomonas cterugirwsa gave the highest production of this enzyme. This. isolate
was selected for protease purifica:tiort, using ammonium sulfate precipitation and ion exchange, DEAE cellulose and gel filtration with sephadex G-100.
Enzyme activity was affected by some antibiotics. The
activity :was reduced with incre ing concentratiohs of these
antibiotics..
