Fourteen isolates were collected from a previous study and all were assigned to be Streptomyces genus, according to physiological and biochemical tests, however all the isolates varied morphologically and exhibited different antimicrobial activity.  All 14 isolates were confirmed Streptomyces by  16S rRNA PCR amplification. Six isolates with high antimicrobial activities were ascertained   Streptomyces spp. by sequencing and phylogenetic analysis. Two isolates among the selected 6 isolates with antimicrobial activity against  E. coli and S. aureus . It recommended to make a complete sequence for 16S  rRNA to detect the  species that

This study was aimed to detect weather copA gene(copper resistance gene ) presence in A.baumannii A92 genome(AbaR genomic islands). The full genomic sequence of A.baumannii A92 not published in NCBI genome similarity was detected between two strains so the sequence of A.baumanniiIS-116(Genebank-AMGF0100000.1 ) was used to design the primers that were used for amplify of copA gene of A.baumannii A92.
Two primers contain two sites for restriction enzymes (KpnI,XohI) and PWSK29 vector were used in the cloning, double digestion has been performed for vector and gene. Then the re-ligation was completed to form recombinant molecule,after that, transformation have been performed for the recombinant molecule by using chemical competent E.coli

         Saccharomyces cerevisiae filtrate showed inhibitory effect against Fusarium spp. when grow in a liquid medium (Sabouraud) with different concentrations (1, 3, 5) %. The higher inhibitory effect against fungus growth was (24.5) mm at (5%) in PDA medium compared with control (36.5) mm during the seventh day propagation. The filtrate of Lactobacillus plantarum isolate was mixed with the PDA medium ,which showed inhibitory effect against Fusarium spp. The concentrated filtrate( one fold) appcarcd a  higher effect against the same fungus compared with un concentrated filtrate one. Saceharomyces cerevisiae and Lactobacillus plantarum

This  paper represent  the second  step  i n  a molecular clon i ng program ai ming to clone large DNA  fi·agmen ts of the sal t tolerant  bermudagrass (Cyrwdon  dactylon  L.)  DNA  usi ng  the  bacteriophage  (EM13L3) as    a vector.

In th is  work, a yield of about  I 00  g bacteriophage  DNA  per one  liter culture.was obtained  with.a  purity ranging between (1.7-1.8). The vector JJNA  v.as  completely   double   digested   with  the  restriction   enzymes llamHI   and  EcoRI,  followed  by  pu

Three strain of Bacillus cereus were obtained from soil sours Laboratories of Biology Department/ College of Science/ University of Baghdad. The bacteria secreted extracellular xylanase in liquid cultur the test ability of xylanase production from these isolates was studied semi quantitative and quantitative screening appeared that Bacillus cereus X3 was the highest xylanase producer. The enzyme was partial purification 191 fold from cultur by reached step by 4 U/mg proteins by ammonium sulfat precipitation 80%, Ion exchang DEAE-cellulos chromatography Characterization study of the partial purifation enzyme revealed that the enzyme had a optimum activity pH8 and activity was stable in the pH rang (8-10) for 30min. maximal activity was attai

The following dilutions -710X46, -610X46, -510X46 of Bacillus thuringiensis were used for bioassay against the different larval instar of the potato tuber moth Phthorimaea operculella by the spraying method, the results showed that there was no significant influence in the percentage of egg hatching in comparison with the control. The sensitivity of larval stages was reduced with the increasing the age and exposure period. The study also showed that the larvae infected with B.t. stopped feeding, movement and a general paralysis causing the death of larva after (24-48) hours , and the larva color was changed from the natural waxy colour to brown finally to the black after death.

The Hbl toxin is a three-component haemolytic complex produced by Bacillus cereus sensu lato strains and implicated as a cause of diarrhoea in B. cereus food poisoning. While the structure of the HblB component of this toxin is known, the structures of the other components are unresolved. Here, we describe the expression of the recombinant HblL1 component and the elucidation of its structure to 1.36 Å. Like HblB, it is a member of the alpha-helical pore-forming toxin family. In comparison to other members of this group, it has an extended hydrophobic beta tongue region that may be involved in pore formation. Molecular docking was used to predict possible interactions between HblL1 and HblB, and suggests a head to tail dimer might f

     In this study, dark and various light qualities (white, red, green, and blue) were applied to evaluate their effects on growth characteristics, chemical content, and callus characteristics of Rosa damascene Mill. and Rosa hybirda L.

   Explant (single-node and shoot tips) cultured on MS media supplemented with sucrose, agar, and plant growth regulators ( Kin 0.5 mg/l and IBA 1 mg/l for whole plant formation experiment or 1 mg/l kin with 0.5 mg/l IBA for callus experiment), incubated in a growth chamber.

   The results of the whole plant formation experiment showed variation in growth characteristics in two types of Rosa, Green and white light caused the height ratio of shoot growth compared wi