The genus Bacillus bacterium isolated from soil and tests their ability to produce glutamic acid, the bacteria are diagnosed by traditional methods and VITEK-2 system. And we studied the optimal conditions for production and try to improve the productivity of the isolation by manipulating the components of the production medium and of the circumstances environmental of production medium. There is 43 isolates of the 70 bacterial isolates belonging to the genus Bacillus depending on phenotypic characteristics of the colonies and microscopic characteristics and found that 13 isolated revert to species B.subtilis depending on physiological and biochemical characteristics. As well as on microscopic and biochemical tests, 13 isolates were subjected to the diagnostic by VITEK-2 system. The results showed that EN5 and EN3 diagnosed as B.subtilis as well as a commercial isolate A1 was also diagnosed as B.subtilis was selected EN3 isolation, as highest productivity (2 mg/ml).then optimal conditions was studied to produce glutamic acid by using the semi synthesis medium contained waste of extract dates (DE) as best Carbone source at 10% and use of ammonium nitrate as the best source of nitrogen at 2% from the production medium, and achieved better productivity of glutamic acid amounted to 7.2 mg/ml, at the temperature 34°C, pH initial 6.5, ventilation and agitation increase concentration of glutamic acid product when the proportion of medium size to the size of the flask 5:1 in the shaker incubator speeds of 180 rpm, for incubated period of 72 hours, were obtained, improvement rate was amounted to 360% from what was produced wild isolation.
Subsurface soil water retention (SWRT) is a recent technology for increasing the crop yield, water use efficiency and then the water productivity with less amount of applied water. The goal of this research was to evaluate the existing of SWRT with the influence of surface and subsurface trickle irrigation on economic water productivity of cucumber crop. Field study was carried out at the Hawr Rajab district of Baghdad governorate from October 1st, to December 31st, 2017. Three experimental treatments were used, treatment plot T1 using SWRT with subsurface trickle irrigation, plot T2 using SWRT with surface trickle irrigation, while plot T3 without using SWRT and using surface tickle irrigation system. The obtained results showed that the e
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(2)
Utilization of bacterial activity for decolorization of coloured products is one the most promising industrial strategy, as an eco-sustainable and cost-competitive alter-native to physicochemical methods. Laccase production from Bacillus sp. was stud-ied for its decolorization influences on different dyes (Indian ink, Brilliant green, Bromothymol blue, Crystal violet, Safranin, Bromophenol blue, Methelen blue, Giemsa stain, Nigrosin, Toluidin blue, Neutral red, Phenol red, Hanna, Blood, Ben-gal rose B, Bromkresol green, 4-Bromoaniline, Aniline blue, 2,6-Dichlorophenol in-vophenol, Curcumin, Acridine orange, Indigo carmine, Xylene cynol FF,10G, Aliza-rine yellow GG and Kongorose). After 5 days of incubation of the spore-bound lac-case wit
... Show MoreThe functional properties of the defatted powder and protein isolate of germinated mung bean seeds were studied and the estimation of the amino acids was carried out. The results showed a significant increase in the values of the amino acids leucine, lysine, phenylalanine and valine. The results of studying the functional properties of the protein isolate and defatted powder showed that there were significant differences between the sample The defatted and protein isolate, if the water absorption capacity of the defatted mung powder was 2.5% water/gm protein and the water absorption capacity of the protein isolate was 3%ml water/g protein, the fat binding capacity of the defatted powder was 0.3 ml fat/g protein and the isolate The ratio was
... Show MoreOne of the significant environmental problems is the pollution of water by dyes;. Biological treatment method was used, which is one of the effective ways to reduce this sort of pollution as it is environment friendly, economic and does not require any expertise. Under controlled conditions, this study estimated the efficacy of dry biomass for Bacillus cereus to reduce Direct Blue 2 dye from the aqueous solution. The optimum conditions such as pH values, contact time and concentration of dyes, were used in this research. The end results showed that the adsorption efficiency, when using a weight of bacterial biomass 0.2 g/50mL, reached 69.2% at a concentration of 10 ppm after one hour at 40°C and pH5. While it reached 5
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This study has been carried out in the animal field of the college of agricultural engineering sciences, university of Baghdad, for the period from 12/15/2021 to 1/26 /2022 for 42 d, to investigate the effect of adding different levels of ellagic acid to the diet of broilers, on some physiological characteristics & oxidation indicators in meat compared to vitamin C in meat, 225 Ross 308 chicks were used, divided randomly to five treatments such us: T1: control group without additives to diet, & the other T2, T3, T4 was added ellagic acid (
... Show MoreFifty isolates of Bacillus spp were obtained from rhizosphere soil of compositae
plant roots. The ability of inulinase production by these isolates was screened.
Bacillus Be9, which isolated from soil of lettuce root, was the highest inulinase
producer; it was identified as Bacillus cereus. Optimal culture medium and
condition for inulinase production were determinatd; the highest inulinase
production was obtained when the bacteria was cultured in inulin medium which
contained 0.5% inulin, 0.4% peptone as carbon and nitrogen source at pH 7.0
inoculated with 1ml of bacterial suspension and incubated at 40˚C for 48hrs.
Select 30 isolate from Bacillus to detect the ability to produce pullulanase enzyme in liquid and solid state fermentation, and use the isolate Bacillus licheniformis (Bs18) because the highest production of enzyme, the optimum condition for the production of enzyme by liquid state fermentation (LSF) in growen with: media contains starch + pullulan as a carbon source, peptone as a nitrogen source, inoculums size 2 ml, and incubated at 40 C° with pH 7 for 48 hrs. In addition pullulanase production by solid state fermentation (SSF) was investigated using isolated Bacillus licheniformis (Bs18). Optimization of process parameters were carried out ,the optimum solid substrate , Temperature , pH , incubation period , inoculation size , hydrat
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Background: The immunogenetic predisposition
may be considered as an important factor for the
development of Type 1 Diabetes Mellitus (T1DM)
in association with the HLA antigens.
Objective:This study was designed to investigate
the role of HLA-class II antigens in the etiology of
type T1DM and in prediction of this disease in
siblings, and its effect on expression of glutamic
acid decarboxylase autoantibodies (GADA).
methods:Sixty children who were newly diagnosed
type 1 diabetes (diagnosed less than five months)
were selected. Their age ranged from 3-17 years.
Another 50 healthy siblings were available for this
study, their ages range from 3-16 years. Eighty
apparently healthy control subjects,