An experiment was conducted to study the effect of four isolates of Pseudomonas spp. on the growth of two plants Radish & Cowpea and on the concentrations of macro elements & microelements . This experiment included two parts , the 1st. part included isolation and characterization of 4 isolates of Pseudomonas bacteria from local Iraqi soils Baghdad . The 2nd part included planting two plants Radish & Cowpea in plastic pots size 5Kg -soil in the green house Biology Dept. College of Science , after planting we added the isolates to the pots , and after 50 days, the growth parameters length , fresh and dry weight , percentage of germination were recorded , and the concentrations of macro elements Mg ,P ,K ,Fe, and Ca ,and microelements Zn ,Cl ,Mo ,Cu ,and Ba. The results showed that the bacterial isolates were effective in promoting the growth of two plants comparing with control plants , the concentrations of maco & microelements were increased in both plants after adding the bacteria to the plants. Results were analyzed statistically using the least significant difference and at the level of probability of 0.05.
Pseudomonas aeruginosa is an opportunistic pathogen that causes a number of infections in immunocompromised patients. This organism appears to improve resistance to many antimicrobial agents and a high percentage of clinical isolates of P. aeruginosa exhibit multidrug resistance (MDR) phenotype . The purpose of this study is to screen the antibiotic susceptibility patterns and the prevalence of qacE delta1 gene among bacterial isolates. Accordingly, 145 samples were collected from different clinical sources from patients who admitted to different hospitals in Baghdad city in a period ranged 23/8/2018-1/1/2019. The isolates were diagnosed as P. aeruginosa based on routine b
... Show MoreBackground: Septicemia is a common condition in children with a resultant high morbidity and mortality. It’s defined as the presence of bacteria in the blood and is often associated with severe infections, the alternative names (Blood poisoning, Bacteremia with sepsis, systemic inflammatory response syndrome).
Objective: To study the etiological agents and antibiotics susceptibility of septicemia in children admitted to Central Teaching Hospitals of Pediatric.
Patients and Methods: A total of 80 patients having signs and symptoms of Septicemia who were admitted to Central Teaching Hospital of Pediatric in Baghdad city. Samples were obtained during the period from the 1st January till the 30 th of Feb
This research included isolation of 18 bacterial isolates from children stool with diarrhea and acute bloody water and under the age of five, if possible get a 9 isolates of the bacterium Escherichia coli and 5 isolates of the bacterium Salmonella typhimurium and 4 isolation of the bacterium Shigella flexneri, subjected all of these isolates tests AGRO and microscopic and biochemical and diagnosed using Vitek2 system. Tested the effectiveness of inhibitory extract alcohol to peel pomegranate Punica granatum L. in the growth of isolates bacterial intestinal and using the diffusion method in drilling Well diffusion as well as determine the focus damper Near Minimum inhibitory concentration MIC and focus killer Near Minimal bactericidal con
... Show MoreThis in order to test the effect of food on growth and fecundity, two kinds of food have been used the algae Scendesmus quadricaudae and fresh water shrimp powder. For two generations, growth and productivity have been followed up. The fresh water shrimp has been noticed as a food better than algae, because it caused recording, for the two generation higher length rate for the weeks of experiment. The individuals length rate at the end of the forth week reached 9.35 and 9.48 mm for the first generation and second generation respectively. The average length weekly increase rate for the first and second generations individuals feeding on dried shrimp was higher through the first and second week compared to what was recorded when feeding alga
... Show MoreSheep are considered as an important part of livestock in the worldwide, particularly in Iraq, as they provide meat, milk, leather, wool, and manure. The present study aim is isolation and identification of staphylococci, enteric bacteria and Pseudomonas spp. Totally, 115 samples were collected from sheep (100 samples were collected from the nasal cavity of local sheep suffering from respiratory infections, and 15 samples were collected from apparently healthy local sheep). All the samples were collected from seven flocks located in Abu Ghraib and Al-Radwaniyah, Baghdad governorate, Iraq. The samples were taken during the period from October 2020 to February 2021. Staphylococcus spp., Pseudomonas spp., and enteric bacteria were detected fi
... Show MoreThe present work aimed to investigate the neuraminidase (nan1) gene expression in 32 different clinical isolates of Pseudomonas aeruginosa to explore the role of the enzyme in different types of infection and might give a better understanding of host cell-pathogens interaction. In addition, the effect of monosaccharide D-mannose on neuraminidase gene expression in eight isolates was studied by utilizing a reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The results demonstrated that the highest expression of nan1 gene was in otitis samples (208,913.81) which were significantly higher than that from other infections (P < 0.01). While, the concentrations of gene copies obtained from urin
... Show MoreBackground: L. sativum, are traditionally used for the treatment of various diseases and thought to have medicinal value. Isolates from many part of the world is now multidrug resistant. Therefore, there is an urgent need to look for and test an alternative herbal drug.
Objective: The present study aimed to evaluate the antibacterial activity of L. Sativum seed extract against multi drug resistant (MDR) and sensitive Pseudomonas aeruginosa clinical isolates.
Subjects and Methods: An ethanolic and aqueous stock extracts were prepared from L. sativum seed plant then serial dilutions were prepared and the obtained concentrations (50, 25, 12.5 and 6.2 mg/ml) were tested against 30 multidrug-resistan
... Show MorePseudomonas aeruginosa is the most common opportunistic pathogen causing morbidity and mortality in hospitalized patients due to its multiple resistance mechanisms. Therefore, as a therapeutic option becomes restricted, the search for a new agent is a preference. So P. aeruginosa is an extremely versatile Gram-negative bacterium capable of thriving in a broad spectrum of environments, and this performs main problems to workers in the field of health. One hundred and fifty samples were collected from different sources from Baghdad hospitals, divided into two main groups: clinical (100) specimens and (50) samples as an environmental, collected from October 2019 to the March 2020. All of these samples were cultured by specific and differential
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