Laboratory experiments were carried out in Technical college AL- Mussiab / Babylon during 2005 to study bioactivity of different concentration from ( aqueous , alcohol and hexane) extracts of bee venom collected from different workers of bees against some bacterial types that cause European Foul – brood Melissococcus plutom , Bacillus alvei and B. letrosporus which had been isolated from infected arched with disease . Two diffusion methods (digging and paper discs) were followed for the extraction of the venom . Results showed that digging diffusion method was more efficient for test of bacterial inhibition which led to increase the activity of bee venom extract with general average 7.90 , 8.85 and 8.19 on M. pluton, B. alvei and B. letrosporus. Alcoholic bee venom extract showed high efficiency in inhibition for the same bacterial species above by digging method too with diameters average 8.15 ,9.76 and 10.59 mm respectively . while aqueous extract was the less efficient in bacterial growth of these bacterial species that reached 7.26 , 7.70 and 6.26 mm respectively in paper discs method compared with control 6.00 mm. On the other hand 40% concentration of bee venom showed high effect of bacterial growth for M.pluton , B. alvei and B. letrosporus with averages 10.60 , 15.60 and 15.60 mm respectively while 1% concentration had no effect in growth of these bacteria compared with control treatment 6.00 mm .Ther is an interaction between the diffusion method, concentration and the kind of the extract . The results reflected that Alcoholic extract at 40% con. by digging method gave a higher inhibition of growth 10.60 , 15.60 and 15.60 mm in M. pluton , B. alvei and B. letrosporus respectively. 40% con. of bee venom compounds however was more effective for bacterial species compared with Oxytetracyclin antibiotic that was very effective in B. alvei and B. letrosporus and less effective in M. pluton bacteria .
ABSTRACT
The multi-drug resistant efflux pump is a glycoprotein pump whose function is to push foreign substances. The efflux pump is found in humans, animals. It also has wide-ranging properties in bacteria and fungi. They are found in all species of bacteria, and efflux pump genes can be found in bacterial chromosomes or mobile genetic elements, such as plasmids. The most sensitive function that leads to a global problem is its resistance to antibiotics in bacterial cells, which increases the ability to bacteria from becoming strong virulence factors that most or all antibiotics cannot kill. It also has othe
... Show MorePseudomonas aerogenosa lipopolysaccharidewas extracted by hot phenol method and purified by gel filtration method using the Sephadex G-200 gel and detected by the limulus amebocyt lysate (EU/ml 0.03)(Wako Chemicals USA, Inc.). The inhibitory effect of partially purified LPS on Candida glabrata yeast was studied in a microdilution method. This study found that LPS has an inhibitory effect on Candida glabrata with the lower concentrations. The inhibitory effect of LPS which treated with heating was studied under boiling and wet heat effect. The toxicity of LPS on Candida glabrata was not affected when treated with heating LPS and the results were similar to those found in untreated LPS
One of the most popular causes for implant infection is dental plaque bacteria. Previous studies have shown the bactericidal effect of CO2 laser irradiation on bacteria associated with soft tissue surrounding the implant materials. No published studies have examined the effect of irradiation by CO2 laser on Streptococcus oralis and Staphylococcus aureus.The aim of this study was to evaluate the bactericidal effect of CO2 laser on bacteria that are causing dental implant infections. This study was carried out on two isolates of bacterial species out of 25 samples, isolated from patients having soft tissue infections around the dental implant. These two pure isolates including Streptococcus oralis and Staphylococcus aureus were identified
... Show MoreWere collected three types of medicinal plants from their natural habitat after Astkhalasalziot volatile manner steam distillation and determine the quality and quantity of vehicles chemical for each of the oils obtained using a technique JC discouraged when you merge oily thyme and lemon grass against bacteria either when using oils in three did not have a different effect
The research deals with the problems of improvisation in two basic subjects and applied a samples and conclusion. The first topic focuses on the concept of improvisation and spontaneity and clarifies the confusion between them. The second topic deals with the foundations and principles on which the actor is based in his artistic preparation for the purpose of developing his improvisational abilities.
The research focuses on clarifying the dimensions of improvisational techniques and its multiplicity in the modern theater. It analyzes the method of improvisation and its use in the construction and composition of the theatrical performance. It deals with two important experiments, such as the experience of the Russian directo
... Show MoreIn this paper we give definitions, properties and examples of the notion of type Ntopological space. Throughout this paper N is a finite positive number, N 2. The task of this paper is to study and investigate some properties of such spaces with the existence of a relation between this space and artificial Neural Networks (ïNN'S), that is we applied the definition of this space in computer field and specially in parallel processing
When the guard honey bees, Apis mellifera L., form a clump at the hive entrance or on the flight board, the oriental hornet, Vespa orientails L., either creeps toward the clump or hovers over it in order to take a bee. Once the hornet creeps, only few bees facing the hornet become alert, rock their heads and antennae, open their wings, and take a posture of defense. The rest of the clump stays listless without any signal of concern. However, the clump stays dense and the defending bees do not detach themselves neither from the rest of the clump nor from each other. For this reason, it is very difficult for the hornet to grab a bee unless the latter makes a “mistake” by detaching herself from other adjacent bees. If the hornet grabs s
... Show Morehe development of multidrug resistance in Gram-negative bacteria is a major problem faced antimicrobial therapy and management of infectious diseases. Too many resistance mechanisms were developed since the antimicrobial agents were produced and actually used. The mechanisms involved in antimicrobial agents' resistance are, modifying enzymes, alteration of the target site of antimicrobials and prevention of antimicrobials accumulation inside the bacterial cells. The latest is accomplished by two mechanisms: alteration of outer membrane permeability and efflux pumps. Efflux pumps are either chromosomal or plasmid-encoded although chromosomal encoded efflux pumps are common in Gram-negative bacteria. Resistance nodulation division (RND) efflu
... Show MoreGlobalization has occupied a great deal of studies, research and literature, in addition to being a phenomenon that has imposed itself firmly on the ground. Globalization is considered the main feature of the current moment in today's world. The world is now transforming in an unprecedented way under noticeable titles of successive waves of knowledge and technology.The current research aims to identify the effects of globalization on the variables and their political, social, media and cultural dimensions, as well as culture of consumption and cultural identity.The theoretical framework included two sections: the first is the concept of globalization, its history and its dimensions, and the second is the modernity in contemporary Europea
... Show More